2024
Insights From Omics in Lyme Disease
Bockenstedt L, Belperron A. Insights From Omics in Lyme Disease. The Journal Of Infectious Diseases 2024, 230: s18-s26. PMID: 39140719, DOI: 10.1093/infdis/jiae250.Peer-Reviewed Original Research
2017
Two Photon Intravital Microscopy of Lyme Borrelia in Mice
Belperron AA, Mao J, Bockenstedt LK. Two Photon Intravital Microscopy of Lyme Borrelia in Mice. Methods In Molecular Biology 2017, 1690: 279-290. PMID: 29032551, DOI: 10.1007/978-1-4939-7383-5_20.Peer-Reviewed Original ResearchVancomycin Reduces Cell Wall Stiffness and Slows Swim Speed of the Lyme Disease Bacterium
Harman MW, Hamby AE, Boltyanskiy R, Belperron AA, Bockenstedt LK, Kress H, Dufresne ER, Wolgemuth CW. Vancomycin Reduces Cell Wall Stiffness and Slows Swim Speed of the Lyme Disease Bacterium. Biophysical Journal 2017, 112: 746-754. PMID: 28256234, PMCID: PMC5340155, DOI: 10.1016/j.bpj.2016.12.039.Peer-Reviewed Original ResearchMeSH KeywordsAnti-Bacterial AgentsBiomechanical PhenomenaBorrelia burgdorferiCell WallLyme DiseaseMechanical PhenomenaMovementVancomycinConceptsCell wall stiffnessCell wallCell stiffnessCell speedLyme disease bacteriumSpirochete survivalPeriplasmic volumeCell shapeFlat wave shapeSwim speedTick hostsCell growthCell morphologyDisease bacteriumVolume regulationHost tissuesTestable hypothesesPrevious mathematical modelingBleb formationSublethal dosesMotilityBiophysical modelSwimming speedBorrelia burgdorferiSlower swim speed
2014
Borrelia burgdorferi Promotes the Establishment of Babesia microti in the Northeastern United States
Dunn JM, Krause PJ, Davis S, Vannier EG, Fitzpatrick MC, Rollend L, Belperron AA, States SL, Stacey A, Bockenstedt LK, Fish D, Diuk-Wasser MA. Borrelia burgdorferi Promotes the Establishment of Babesia microti in the Northeastern United States. PLOS ONE 2014, 9: e115494. PMID: 25545393, PMCID: PMC4278703, DOI: 10.1371/journal.pone.0115494.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBabesia microtiBabesiosisBorrelia burgdorferiCoinfectionIxodesNew EnglandPeromyscusConceptsB. microtiB. burgdorferiBabesia microtiLyme diseaseRespective causative agentsSpread of babesiosisI. scapularis larvaeInfected micePrimary reservoir hostHigh prevalenceLeucopus miceMiceHuman babesiosisBorrelia burgdorferiLaboratory dataLarval burdenCausative agentMicrotiBurgdorferiCoinfectionReservoir hostsDiseaseR0 modelBasic reproduction numberEnzootic cycleDual role for Fcγ receptors in host defense and disease in Borrelia burgdorferi-infected mice
Belperron AA, Liu N, Booth CJ, Bockenstedt LK. Dual role for Fcγ receptors in host defense and disease in Borrelia burgdorferi-infected mice. Frontiers In Cellular And Infection Microbiology 2014, 4: 75. PMID: 24967215, PMCID: PMC4052197, DOI: 10.3389/fcimb.2014.00075.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBacterial LoadBorrelia burgdorferiDisease Models, AnimalLyme DiseaseMiceMice, Inbred C57BLMice, KnockoutReceptors, IgGConceptsInnate immune cellsWild-type miceB. burgdorferi infectionB. burgdorferi-infected miceBurgdorferi-infected miceImmune cellsBurgdorferi infectionType micePathogen burdenHost defenseFc receptor common gamma chainIgG-mediated clearanceC-reactive proteinBorrelia burgdorferiToll-Like Receptor InteractionsSingle knockout miceCommon gamma chainAcute phaseInfected jointsArthritis severityMolecule MyD88Inflammatory cytokinesInflammatory pathwaysIL-1betaReactive proteinWhat ticks do under your skin: two-photon intravital imaging of Ixodes scapularis feeding in the presence of the lyme disease spirochete.
Bockenstedt LK, Gonzalez D, Mao J, Li M, Belperron AA, Haberman A. What ticks do under your skin: two-photon intravital imaging of Ixodes scapularis feeding in the presence of the lyme disease spirochete. The Yale Journal Of Biology And Medicine 2014, 87: 3-13. PMID: 24600332, PMCID: PMC3941458.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArachnid VectorsBorrelia burgdorferiFeeding BehaviorGreen Fluorescent ProteinsHost-Parasite InteractionsHost-Pathogen InteractionsIxodesLyme DiseaseMiceMice, Inbred BALB CMice, Inbred C57BLMice, KnockoutMicroscopy, ConfocalMicroscopy, Electron, ScanningMicroscopy, Fluorescence, MultiphotonNymphSkinTick InfestationsTime Factors
2012
Spirochete antigens persist near cartilage after murine Lyme borreliosis therapy
Bockenstedt LK, Gonzalez DG, Haberman AM, Belperron AA. Spirochete antigens persist near cartilage after murine Lyme borreliosis therapy. Journal Of Clinical Investigation 2012, 122: 2652-2660. PMID: 22728937, PMCID: PMC3386809, DOI: 10.1172/jci58813.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnti-Bacterial AgentsAntigens, BacterialArthritis, InfectiousBacterial LoadBorrelia burgdorferiCartilageCeftriaxoneDoxycyclineEar, ExternalFemaleFluorescence Recovery After PhotobleachingGreen Fluorescent ProteinsJoint CapsuleLyme DiseaseMiceMice, Inbred C3HMice, Inbred C57BLMice, KnockoutMicroscopy, Fluorescence, MultiphotonMyeloid Differentiation Factor 88PatellaRecombinant ProteinsConceptsNaive miceAntibiotic treatmentIntravital microscopyAntibiotic-refractory Lyme arthritisLyme diseaseTNF-α productionBorrelia burgdorferi antigensB. burgdorferi antigensSpirochete antigenTLR responsivenessInflammatory arthritisAntibiotic therapyLyme arthritisWT miceMusculoskeletal symptomsAntigens persistSlow resolutionImmunodeficient miceMouse modelTissue transplantsPathogen burdenSpirochete DNAInfectious spirochetesLyme borreliosisMiceThe heterogeneous motility of the Lyme disease spirochete in gelatin mimics dissemination through tissue
Harman MW, Dunham-Ems SM, Caimano MJ, Belperron AA, Bockenstedt LK, Fu HC, Radolf JD, Wolgemuth CW. The heterogeneous motility of the Lyme disease spirochete in gelatin mimics dissemination through tissue. Proceedings Of The National Academy Of Sciences Of The United States Of America 2012, 109: 3059-3064. PMID: 22315410, PMCID: PMC3286914, DOI: 10.1073/pnas.1114362109.Peer-Reviewed Original Research
2009
The Caspase 1 Inflammasome Is Not Required for Control of Murine Lyme Borreliosis
Liu N, Belperron AA, Booth CJ, Bockenstedt LK. The Caspase 1 Inflammasome Is Not Required for Control of Murine Lyme Borreliosis. Infection And Immunity 2009, 77: 3320-3327. PMID: 19487481, PMCID: PMC2715671, DOI: 10.1128/iai.00100-09.Peer-Reviewed Original ResearchConceptsCaspase-1 inflammasomeCaspase-1Immune responseHost defenseLyme borreliosisToll-like receptor-mediated responsesDay 14 postinfectionPrevalence of arthritisT cell responsesApoptosis-associated speck-like proteinMild transient elevationBorrelia burgdorferiMurine Lyme borreliosisReceptor-mediated responsesCaspase-1 deficiencyC-terminal caspase recruitment domainSpeck-like proteinAbility of macrophagesEnzyme caspase-1IL-18Humoral immunityInterleukin-1betaTransient elevationPathogen burdenInflammasome
2007
Marginal Zone B-Cell Depletion Impairs Murine Host Defense against Borrelia burgdorferi Infection
Belperron AA, Dailey CM, Booth CJ, Bockenstedt LK. Marginal Zone B-Cell Depletion Impairs Murine Host Defense against Borrelia burgdorferi Infection. Infection And Immunity 2007, 75: 3354-3360. PMID: 17470546, PMCID: PMC1932939, DOI: 10.1128/iai.00422-07.Peer-Reviewed Original ResearchConceptsT-cell-independent antibodiesCell-depleted miceSplenic CD4Cell depletionPathogen burdenSplenic T cell responsesHost defenseT-cell activation markersC3H/HeJ miceImmunoglobulin M titersInfected control miceMurine host defensePathogen-specific IgMT cell responsesMarginal zone B cellsBlood-borne antigensBlood-borne pathogensWeeks of infectionB cell subsetsImmune mouse serumBorrelia burgdorferi infectionImportant host defenseSevere arthritisActivation markersControl mice
2003
Humoral Immunity Reflects Altered T Helper Cell Bias in Borrelia burgdorferi-Infected γδ T-Cell-Deficient Mice
Bockenstedt LK, Shanafelt MC, Belperron A, Mao J, Barthold SW. Humoral Immunity Reflects Altered T Helper Cell Bias in Borrelia burgdorferi-Infected γδ T-Cell-Deficient Mice. Infection And Immunity 2003, 71: 2938-2940. PMID: 12704174, PMCID: PMC153287, DOI: 10.1128/iai.71.5.2938-2940.2003.Peer-Reviewed Original ResearchConceptsΓδ T-cell-deficient miceT cell-deficient miceGamma delta T cellsGamma delta T-cell functionGamma delta TBorrelia burgdorferiDelta T cellsMurine Lyme borreliosisT cell functionHumoral immunityHumoral responseAntibody isotypesT cellsLyme borreliosisCell biasDisease susceptibilityArthropod transmissionBurgdorferi