2017
DRP1 Suppresses Leptin and Glucose Sensing of POMC Neurons
Santoro A, Campolo M, Liu C, Sesaki H, Meli R, Liu ZW, Kim JD, Diano S. DRP1 Suppresses Leptin and Glucose Sensing of POMC Neurons. Cell Metabolism 2017, 25: 647-660. PMID: 28190775, PMCID: PMC5366041, DOI: 10.1016/j.cmet.2017.01.003.Peer-Reviewed Original ResearchConceptsPeroxisome proliferator-activated receptorPOMC neuronsLeptin sensitivityHypothalamic pro-opiomelanocortin (POMC) neuronsPro-opiomelanocortin (POMC) neuronsCounter-regulatory responseProliferator-activated receptorMitochondrial sizeFed miceGlucoprivic stimuliNeuronal activationFl/Glucose metabolismMetabolic environmentNeuronsFasted animalsIntracellular mechanismsReduced expressionGlucose responsivenessGreater activationInducible deletionROS productionMiceStrong inhibitionMitochondrial fission regulator
2014
Leptin signaling in astrocytes regulates hypothalamic neuronal circuits and feeding
Kim JG, Suyama S, Koch M, Jin S, Argente-Arizon P, Argente J, Liu ZW, Zimmer MR, Jeong JK, Szigeti-Buck K, Gao Y, Garcia-Caceres C, Yi CX, Salmaso N, Vaccarino FM, Chowen J, Diano S, Dietrich MO, Tschöp MH, Horvath TL. Leptin signaling in astrocytes regulates hypothalamic neuronal circuits and feeding. Nature Neuroscience 2014, 17: 908-910. PMID: 24880214, PMCID: PMC4113214, DOI: 10.1038/nn.3725.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAstrocytesCell CountEatingExcitatory Postsynaptic PotentialsGlial Fibrillary Acidic ProteinHypothalamusImmunohistochemistryIn Situ HybridizationLeptinMaleMelanocortinsMiceMice, KnockoutMicroscopy, ElectronNerve NetNeuronsPrimary Cell CulturePro-OpiomelanocortinPulmonary Gas ExchangeReal-Time Polymerase Chain ReactionRNA, MessengerSignal Transduction
2009
Prolylcarboxypeptidase regulates food intake by inactivating α-MSH in rodents
Wallingford N, Perroud B, Gao Q, Coppola A, Gyengesi E, Liu ZW, Gao XB, Diament A, Haus KA, Shariat-Madar Z, Mahdi F, Wardlaw SL, Schmaier AH, Warden CH, Diano S. Prolylcarboxypeptidase regulates food intake by inactivating α-MSH in rodents. Journal Of Clinical Investigation 2009, 119: 2291-2303. PMID: 19620781, PMCID: PMC2719925, DOI: 10.1172/jci37209.Peer-Reviewed Original ResearchConceptsFood intakeHigh-fat diet-induced obesityReduced body fatRegular chow dietDiet-induced obesityPRCP activityWild-type controlsChow dietMelanocortin signalingObese miceWeight maintenanceReal-time PCRAxon terminalsBody fatNeuronal populationsΑ-MSHBrain tissueMRNA expressionMouse strainsSmall molecule protease inhibitorsElevated levelsVivo activityProlylcarboxypeptidaseProtease inhibitorsHypothalamus
2006
Leptin Receptor Signaling in Midbrain Dopamine Neurons Regulates Feeding
Hommel JD, Trinko R, Sears RM, Georgescu D, Liu ZW, Gao XB, Thurmon JJ, Marinelli M, DiLeone RJ. Leptin Receptor Signaling in Midbrain Dopamine Neurons Regulates Feeding. Neuron 2006, 51: 801-810. PMID: 16982424, DOI: 10.1016/j.neuron.2006.08.023.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAnimalsDopamineEatingFeeding BehaviorGene ExpressionIn Situ Hybridization, FluorescenceIn Vitro TechniquesInfusions, IntravenousLeptinMesencephalonMiceMice, Inbred C57BLMotor ActivityNeuronsPhosphorylationRatsRats, Sprague-DawleyReceptors, Cell SurfaceReceptors, LeptinRNA InterferenceRNA, MessengerSignal TransductionSTAT3 Transcription FactorVentral Tegmental AreaConceptsVentral tegmental areaVTA dopamine neuronsDopamine neuronsFood intakePeripheral metabolic signalsNormal food intakeMidbrain dopamine neuronsLeptin receptor signalingLeptin hormoneTegmental areaLEPR expressionLeptin receptor functionLocomotor activityPalatable foodDirect administrationReceptor functionFiring rateReceptor signalingDirect actionNeuronsLEPR mRNAFunctional evidenceLeptinRNAi-mediated knockdownMetabolic signals