2009
CASK Deletion in Intestinal Epithelia Causes Mislocalization of LIN7C and the DLG1/Scrib Polarity Complex without Affecting Cell Polarity
Lozovatsky L, Abayasekara N, Piawah S, Walther Z. CASK Deletion in Intestinal Epithelia Causes Mislocalization of LIN7C and the DLG1/Scrib Polarity Complex without Affecting Cell Polarity. Molecular Biology Of The Cell 2009, 20: 4489-4499. PMID: 19726564, PMCID: PMC2770937, DOI: 10.1091/mbc.e09-04-0280.Peer-Reviewed Original ResearchConceptsEpidermal growth factor receptorEpithelial polarityNormal localizationBasolateral membranePolarity complexMammalian orthologsCell polarityCaenorhabditis elegansMAGUK familyProtein complexesIntestinal epitheliumGrowth factor receptorLIN7CBasolateral localizationComplete knockoutCASK expressionTumor suppressorSubcellular distributionDlg1CASK deletionFactor receptorImmunofluorescence analysisErbB-2Intestinal homeostasisAppropriate localization
2000
hCASK and hDlg Associate in Epithelia, and Their Src Homology 3 and Guanylate Kinase Domains Participate in Both Intramolecular and Intermolecular Interactions*
Nix S, Chishti A, Anderson J, Walther Z. hCASK and hDlg Associate in Epithelia, and Their Src Homology 3 and Guanylate Kinase Domains Participate in Both Intramolecular and Intermolecular Interactions*. Journal Of Biological Chemistry 2000, 275: 41192-41200. PMID: 10993877, DOI: 10.1074/jbc.m002078200.Peer-Reviewed Original ResearchConceptsHuman CASKGUK domainGuanylate kinasePlasma membraneSrc homology 3 domainSrc homology 3Two-hybrid assayGuanylate kinase domainBasolateral plasma membraneTwo-hybridHomology 3Protein binding assaysMAGUK proteinsMultiprotein complexesSH3 domainHuman p55Kinase domainScaffold assemblyIntestinal cell lineMAGUKsHDlgHEK293 cellsImmunofluorescence microscopyIntramolecular bindingSpecialized regions
1998
Human CASK/LIN-2 Binds Syndecan-2 and Protein 4.1 and Localizes to the Basolateral Membrane of Epithelial Cells
Cohen A, Wood D, Marfatia S, Walther Z, Chishti A, Anderson J. Human CASK/LIN-2 Binds Syndecan-2 and Protein 4.1 and Localizes to the Basolateral Membrane of Epithelial Cells. Journal Of Cell Biology 1998, 142: 129-138. PMID: 9660868, PMCID: PMC2133028, DOI: 10.1083/jcb.142.1.129.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalcium-Calmodulin-Dependent Protein KinasesCarrier ProteinsCloning, MolecularCytoskeletal ProteinsEpithelial CellsGuanylate KinasesHelminth ProteinsHumansMembrane GlycoproteinsMembrane ProteinsMicrofilament ProteinsNeuropeptidesNucleoside-Phosphate KinaseProteoglycansRabbitsRatsRecombinant Fusion ProteinsSpectrinSyndecan-2ConceptsProtein 4.1LIN-2Membrane domainsRas/MAP kinase pathwayMembrane-associated guanylate kinase (MAGUK) familySyndecan-2Membrane-associated guanylate kinaseVulval precursor cellsTwo-hybrid screenBasal plasma membrane domainsPlasma membrane domainsExtracellular matrixCASK/LINHeparan sulfate proteoglycan syndecan-2Basal membrane domainsMAP kinase pathwayProteoglycan syndecan-2Receptor tyrosine kinasesDifferent epithelial cell typesEpithelial cell typesLET-23Caenorhabditis elegansPDZ domainActin cytoskeletonGuanylate kinase