Protein kinase Cα–mediated phosphorylation of Twist1 at Ser-144 prevents Twist1 ubiquitination and stabilizes it
Tedja R, Roberts CM, Alvero AB, Cardenas C, Yang-Hartwich Y, Spadinger S, Pitruzzello M, Yin G, Glackin CA, Mor G. Protein kinase Cα–mediated phosphorylation of Twist1 at Ser-144 prevents Twist1 ubiquitination and stabilizes it. Journal Of Biological Chemistry 2019, 294: 5082-5093. PMID: 30733340, PMCID: PMC6442047, DOI: 10.1074/jbc.ra118.005921.Peer-Reviewed Original ResearchConceptsProtein kinase CPhosphorylation sitesHelix transcription factorPhosphorylation of Twist1Candidate phosphorylation sitesProtein kinase CαCombination of immunoblottingUbiquitination eventsTwist1 phosphorylationTranscription factorsEmbryonic developmentKnockout experimentsSer-144CRISPR/Twist1 proteinKinase CInvasive phenotypeAdult cellsPhosphorylationTwist1UbiquitinationCancer developmentCell linesTwist1 expressionPKCαp53-Pirh2 Complex Promotes Twist1 Degradation and Inhibits EMT
Yang-Hartwich Y, Tedja R, Roberts C, Goodner-Bingham J, Cardenas C, Gurea M, Sumi NJ, Alvero AB, Glackin CA, Mor G. p53-Pirh2 Complex Promotes Twist1 Degradation and Inhibits EMT. Molecular Cancer Research 2019, 17: molcanres.0238.2018. PMID: 30131448, PMCID: PMC6800184, DOI: 10.1158/1541-7786.mcr-18-0238.Peer-Reviewed Original ResearchConceptsEpithelial-mesenchymal transitionTwist1 degradationInvasive cancer phenotypeEMT-inducing transcription factorsAbility of p53Tumor suppressor geneTumor cell invasivenessWild-type p53Proteasomal degradationTranscription factorsTwist1 proteinSuppressor geneEpithelial phenotypeInhibits epithelial-mesenchymal transitionCancer phenotypeMolecular levelCell invasivenessCancer progressionCancer metastasisWt p53Twist1P53Metastatic processTumor progressionNew insights