2000
Direct Interaction between Endothelial Nitric-oxide Synthase and Dynamin-2 IMPLICATIONS FOR NITRIC-OXIDE SYNTHASE FUNCTION*
Cao S, Yao J, McCabe T, Yao Q, Katusic Z, Sessa W, Shah V. Direct Interaction between Endothelial Nitric-oxide Synthase and Dynamin-2 IMPLICATIONS FOR NITRIC-OXIDE SYNTHASE FUNCTION*. Journal Of Biological Chemistry 2000, 276: 14249-14256. PMID: 11120737, DOI: 10.1074/jbc.m006258200.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaBlotting, WesternCalcimycinCattleCell LineDose-Response Relationship, DrugDynamin IDynaminsEndothelium, VascularGlutathione TransferaseGolgi ApparatusGTP PhosphohydrolasesIonophoresKineticsMicroscopy, ConfocalMicroscopy, FluorescenceNitric Oxide SynthaseNitric Oxide Synthase Type IIIPrecipitin TestsProtein BindingProtein BiosynthesisRatsRecombinant Fusion ProteinsTransfectionConceptsDynamin 2Bovine aortic endothelial cellsRecombinant eNOS proteinDirect protein-protein interactionDouble-label confocal immunofluorescenceProtein-protein interactionsSpecific protein interactionsDirect interactionClone 9 cellsEndothelial nitric oxide synthaseMembrane compartmentsLarge GTPaseGlutathione S-transferaseProtein interactionsNovel functionDynaminECV-304 cellsSynthase functionIntracellular signalsMembrane distributionConfocal immunofluorescenceFluorescent proteinENOS regulationGreen fluorescentProtein
1999
Induction of Nitric Oxide Synthase mRNA by Shear Stress Requires Intracellular Calcium and G-protein Signals and Is Modulated by PI 3 Kinase
Malek A, Jiang L, Lee I, Sessa W, Izumo S, Alper S. Induction of Nitric Oxide Synthase mRNA by Shear Stress Requires Intracellular Calcium and G-protein Signals and Is Modulated by PI 3 Kinase. Biochemical And Biophysical Research Communications 1999, 254: 231-242. PMID: 9920763, DOI: 10.1006/bbrc.1998.9921.Peer-Reviewed Original ResearchConceptsNitric oxide synthase mRNAPTX-sensitive G proteinsENOS mRNA levelsENOS mRNABovine aortic endothelial cellsIntracellular calciumPertussis toxinMRNA upregulationEndothelial nitric oxide synthase (eNOS) mRNAMRNA levelsEndothelin-1 mRNACalmodulin inhibitor WENOS gene promoterG proteinsSynthase mRNAAortic endothelial cellsTime-dependent increaseTyrosine kinase inhibitor herbimycin ACalcium entryBAPTA-AMInhibitor WEndothelial cellsTyrosine kinase activityMicrotubule integrityLaminar fluid shear stress
1996
Endothelial Nitric Oxide Synthase Is Regulated by Tyrosine Phosphorylation and Interacts with Caveolin-1*
García-Cardeña G, Fan R, Stern D, Liu J, Sessa W. Endothelial Nitric Oxide Synthase Is Regulated by Tyrosine Phosphorylation and Interacts with Caveolin-1*. Journal Of Biological Chemistry 1996, 271: 27237-27240. PMID: 8910295, DOI: 10.1074/jbc.271.44.27237.Peer-Reviewed Original ResearchConceptsNovel regulatory mechanismTyrosine phosphorylationCaveolin-1Bovine aortic endothelial cellsRegulatory mechanismsProtein tyrosine phosphatase inhibitorCaveolin-interacting proteinsPhosphoamino acid analysisTyrosine phosphatase inhibitorTreatment of BAECBovine lung microvascular endothelial cellsEndothelial nitric oxide synthaseSubcellular traffickingPhosphatase inhibitorCoat proteinEndothelial cellsMetabolic labelingSodium orthovanadatePhosphorylationCaveolaeAortic endothelial cellsLung microvascular endothelial cellsProteinAcid analysisImmunoprecipitation
1995
Functional Analysis of the Human Endothelial Nitric Oxide Synthase Promoter SP1 AND GATA FACTORS ARE NECESSARY FOR BASAL TRANSCRIPTION IN ENDOTHELIAL CELLS (∗)
Zhang R, Min W, Sessa W. Functional Analysis of the Human Endothelial Nitric Oxide Synthase Promoter SP1 AND GATA FACTORS ARE NECESSARY FOR BASAL TRANSCRIPTION IN ENDOTHELIAL CELLS (∗). Journal Of Biological Chemistry 1995, 270: 15320-15326. PMID: 7541039, DOI: 10.1074/jbc.270.25.15320.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid OxidoreductasesAnimalsAortaBase SequenceBinding SitesCattleDNA PrimersDNA-Binding ProteinsEndothelium, VascularGATA2 Transcription FactorGene ExpressionHumansLuciferasesMolecular Sequence DataMutagenesis, Site-DirectedNitric Oxide SynthasePolymerase Chain ReactionPromoter Regions, GeneticRecombinant ProteinsRestriction MappingSp1 Transcription FactorTranscription FactorsTranscription, GeneticTransfectionConceptsBovine aortic endothelial cellsPromoter activitySp1 sitesBasal transcriptionGATA siteFurther deletionNuclear extractsENOS promoter activityAntibody supershift analysisDNA-protein complexesLuciferase activityENOS promoterLuc constructGel shift analysisSp-1 sitesBasal promoter activityLuciferase reporter geneSynthase gene expressionSp1 mutantGATA factorsNuclear proteinsGATA-2Mutant constructsReporter geneGel shift
1990
Inhibition by l‐glutamine of the release of endothelium‐derived relaxing factor from cultured endothelial cells
Hecker M, Mitchell J, Swierkosz T, Sessa W, Vane J. Inhibition by l‐glutamine of the release of endothelium‐derived relaxing factor from cultured endothelial cells. British Journal Of Pharmacology 1990, 101: 237-239. PMID: 2257431, PMCID: PMC1917688, DOI: 10.1111/j.1476-5381.1990.tb12693.x.Peer-Reviewed Original Research