2024
RluA is the major mRNA pseudouridine synthase in Escherichia coli
Schaening-Burgos C, LeBlanc H, Fagre C, Li G, Gilbert W. RluA is the major mRNA pseudouridine synthase in Escherichia coli. PLOS Genetics 2024, 20: e1011100. PMID: 39241085, PMCID: PMC11421799, DOI: 10.1371/journal.pgen.1011100.Peer-Reviewed Original ResearchMeSH KeywordsEscherichia coliEscherichia coli ProteinsIntramolecular TransferasesNucleic Acid ConformationPhosphorus-Oxygen LyasesPseudouridineRNA Processing, Post-TranscriptionalRNA, BacterialRNA, MessengerRNA, Ribosomal, 23SRNA, TransferConceptsPseudouridine synthasesHigh-confidence sitesMRNA-modifying enzymesE. coli mRNAsStructure probing dataIdentified target sitesTarget siteDiverse eukaryotesBacterial mRNAsRNA modificationsRluAEscherichia coliSecondary structureE. coliTRNAPseudouridineRRNAStructural motifsMRNAModification capacityRecognition elementsSynthaseRNASequenceEukaryotesRecent developments, opportunities, and challenges in the study of mRNA pseudouridylation
Gilbert W. Recent developments, opportunities, and challenges in the study of mRNA pseudouridylation. RNA 2024, 30: rna.079975.124. PMID: 38531650, PMCID: PMC11019745, DOI: 10.1261/rna.079975.124.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsHumansIntramolecular TransferasesProtein BindingProtein BiosynthesisPseudouridineResearchRNA PrecursorsRNA Processing, Post-TranscriptionalRNA, MessengerRNA-Binding ProteinsTranscription, GeneticBeyond reader proteins: RNA binding proteins and RNA modifications in conversation to regulate gene expression
Fagre C, Gilbert W. Beyond reader proteins: RNA binding proteins and RNA modifications in conversation to regulate gene expression. Wiley Interdisciplinary Reviews - RNA 2024, 15: e1834. PMID: 38444048, DOI: 10.1002/wrna.1834.Peer-Reviewed Original ResearchMeSH KeywordsGene ExpressionRNARNA Processing, Post-TranscriptionalRNA, MessengerRNA-Binding ProteinsConceptsRNA-binding proteinsRNA modificationsMRNA modificationRNA structureRNA interactionsRNA Processing > RNA EditingRNA-binding protein's bindingEffects of RNA modificationsBinding proteinGene expressionRNA-protein interactomePost-transcriptional mRNA modificationDihydrouridine (DRegulate gene expressionRNA substratesProtein-RNARNA editingRNA functionReader proteinsModification statusN1-methyladenosineCellular machinerySequence contextSecondary structureBiochemical properties
2016
Messenger RNA modifications: Form, distribution, and function
Gilbert WV, Bell TA, Schaening C. Messenger RNA modifications: Form, distribution, and function. Science 2016, 352: 1408-1412. PMID: 27313037, PMCID: PMC5094196, DOI: 10.1126/science.aad8711.Peer-Reviewed Original ResearchMeSH Keywords5-MethylcytosineAdenosineAnimalsEpigenesis, GeneticGene Expression Regulation, DevelopmentalHumansMethylationMethyltransferasesPseudouridineRNA Processing, Post-TranscriptionalRNA, MessengerTranscriptomeConceptsStable noncoding RNAsMRNA target siteOrganismal phenotypesDevelopmental regulatorsNoncoding RNAsMRNA modificationMolecular consequencesMessenger RNAMolecular effectsRNATarget siteRecent technical advancesDistinct modificationsSparse modificationEpitranscriptomeSplicingMethyladenosineRegulatorSpeciesTechnical advancesModificationPhenotypeRegulationFunctionCells