1985
Cross-linking studies with the uvrA and uvrB proteins of E. coli
Strike P, Rupp W. Cross-linking studies with the uvrA and uvrB proteins of E. coli. Mutation Research/Fundamental And Molecular Mechanisms Of Mutagenesis 1985, 145: 43-48. PMID: 3156273, DOI: 10.1016/0167-8817(85)90038-0.Peer-Reviewed Original Research
1983
A novel repair enzyme: UVRABC excision nuclease of Escherichia coli cuts a DNA strand on both sides of the damaged region
Sancar A, Rupp W. A novel repair enzyme: UVRABC excision nuclease of Escherichia coli cuts a DNA strand on both sides of the damaged region. Cell 1983, 33: 249-260. PMID: 6380755, DOI: 10.1016/0092-8674(83)90354-9.Peer-Reviewed Original ResearchConceptsUvrABC nucleaseFifth phosphodiester bond 3Escherichia coliDNA strandsPhosphodiester bond 3UvrABC excision nucleasePyrimidine dimersUV-induced mutagenesisUvrC proteinUV-irradiated DNAExcision nucleaseRepair enzymesPhosphodiester bondNucleaseEnzymeProteinBond 3DNAColiUvrBStrandsUvrAMutagenesisDimersSame mechanism
1982
LexA protein inhibits transcription of the E. coli uvrA gene in vitro
Sancar A, Sancar G, Rupp W, Little J, Mount D. LexA protein inhibits transcription of the E. coli uvrA gene in vitro. Nature 1982, 298: 96-98. PMID: 6283374, DOI: 10.1038/298096a0.Peer-Reviewed Original Research
1981
E. coli uvrB protein binds to DNA in the presence of uvrA protein
Kacinski B, Rupp W. E. coli uvrB protein binds to DNA in the presence of uvrA protein. Nature 1981, 294: 480-481. PMID: 6273745, DOI: 10.1038/294480a0.Peer-Reviewed Original ResearchConceptsGene productsMulti-protein complexesUvrC gene productDNA-binding proteinsNucleotide excision repairPresence of UvrAUvrA proteinEndonucleolytic incisionProtein bindsExcision repairUvrBUvrADNADirect interactionProteinBulky lesionsPyrimidine dimersGenesTight bindingBindsBindingRepairEarly stagesVivoPurificationIdentification of the uvrA gene product
Sancar A, Wharton R, Seltzer S, Kacinski B, Clarke N, Rupp W. Identification of the uvrA gene product. Journal Of Molecular Biology 1981, 148: 45-62. PMID: 6273577, DOI: 10.1016/0022-2836(81)90234-5.Peer-Reviewed Original ResearchConceptsUvrA proteinUvrA geneSsb geneMr proteinE. coli genetic mapDirection of transcriptionEscherichia coli UvrAMaxicell procedureGenetic mapNonsense codonUvrA gene productGene productsSmall proteinsRestriction mappingDouble-stranded DNAGenesRecombinant plasmidProteinUvrANormal cellsPremature terminationPolypeptideDNAPlasmidSubsequent gel filtrationA general approach for purifying proteins encoded by cloned genes without using a functional assay: isolation of the uvrA gene product from radiolabeled maxicells
Kacinski B, Sancar A, Rupp W. A general approach for purifying proteins encoded by cloned genes without using a functional assay: isolation of the uvrA gene product from radiolabeled maxicells. Nucleic Acids Research 1981, 9: 4495-4508. PMID: 6272208, PMCID: PMC327453, DOI: 10.1093/nar/9.18.4495.Peer-Reviewed Original ResearchConceptsUvrA geneGene productsFunctional assaysIntrinsic endonuclease activityUvrA proteinLexA proteinRapid purification procedureUV-irradiated DNAMulticopy plasmidUvrA gene productEndonuclease activityRecA cellsUvrAGenesEnzyme assaysProteinMaxicellsATPase activityE. coliSpecific labelingDNASpecific cuttingPurification procedureAssaysRepressor
1979
Cloning of uvrA, lexC and ssb genes of Escherichia coli
Sancar A, Rupp W. Cloning of uvrA, lexC and ssb genes of Escherichia coli. Biochemical And Biophysical Research Communications 1979, 90: 123-129. PMID: 387033, DOI: 10.1016/0006-291x(79)91598-5.Peer-Reviewed Original Research