A general approach for purifying proteins encoded by cloned genes without using a functional assay: isolation of the uvrA gene product from radiolabeled maxicells
Kacinski B, Sancar A, Rupp W. A general approach for purifying proteins encoded by cloned genes without using a functional assay: isolation of the uvrA gene product from radiolabeled maxicells. Nucleic Acids Research 1981, 9: 4495-4508. PMID: 6272208, PMCID: PMC327453, DOI: 10.1093/nar/9.18.4495.Peer-Reviewed Original ResearchConceptsUvrA geneGene productsFunctional assaysIntrinsic endonuclease activityUvrA proteinLexA proteinRapid purification procedureUV-irradiated DNAMulticopy plasmidUvrA gene productEndonuclease activityRecA cellsUvrAGenesEnzyme assaysProteinMaxicellsATPase activityE. coliSpecific labelingDNASpecific cuttingPurification procedureAssaysRepressor