2015
Adaptive Evolution of Eel Fluorescent Proteins from Fatty Acid Binding Proteins Produces Bright Fluorescence in the Marine Environment
Gruber DF, Gaffney JP, Mehr S, DeSalle R, Sparks JS, Platisa J, Pieribone VA. Adaptive Evolution of Eel Fluorescent Proteins from Fatty Acid Binding Proteins Produces Bright Fluorescence in the Marine Environment. PLOS ONE 2015, 10: e0140972. PMID: 26561348, PMCID: PMC4641735, DOI: 10.1371/journal.pone.0140972.Peer-Reviewed Original ResearchConceptsAcid-binding proteinFluorescent proteinFatty acid-binding proteinDuplication eventsGene duplication eventsStrong positive selectionNew fluorescent proteinsMarine environmentAdaptive evolutionEvolutionary switchCryptic genusVertebrate ordersVertebrate taxaPhylogenetic reconstructionProtein familyPhylogenetic analysisSequence motifsSister groupEvolutionary adaptationTranscriptomic analysisPositive selectionFatty acid binding proteinAcid binding proteinBinding proteinBright green fluorescence
2013
Transcriptome deep-sequencing and clustering of expressed isoforms from Favia corals
Pooyaei Mehr SF, DeSalle R, Kao HT, Narechania A, Han Z, Tchernov D, Pieribone V, Gruber DF. Transcriptome deep-sequencing and clustering of expressed isoforms from Favia corals. BMC Genomics 2013, 14: 546. PMID: 23937070, PMCID: PMC3751062, DOI: 10.1186/1471-2164-14-546.Peer-Reviewed Original Research
2012
A Fluorescent, Genetically-Encoded Voltage Probe Capable of Resolving Action Potentials
Barnett L, Platisa J, Popovic M, Pieribone VA, Hughes T. A Fluorescent, Genetically-Encoded Voltage Probe Capable of Resolving Action Potentials. PLOS ONE 2012, 7: e43454. PMID: 22970127, PMCID: PMC3435330, DOI: 10.1371/journal.pone.0043454.Peer-Reviewed Original ResearchGenetically encoded fluorescent voltage sensors using the voltage-sensing domain of Nematostella and Danio phosphatases exhibit fast kinetics
Baker BJ, Jin L, Han Z, Cohen LB, Popovic M, Platisa J, Pieribone V. Genetically encoded fluorescent voltage sensors using the voltage-sensing domain of Nematostella and Danio phosphatases exhibit fast kinetics. Journal Of Neuroscience Methods 2012, 208: 190-196. PMID: 22634212, PMCID: PMC3398169, DOI: 10.1016/j.jneumeth.2012.05.016.Peer-Reviewed Original Research
2010
A new bright green‐emitting fluorescent protein – engineered monomeric and dimeric forms
Ilagan RP, Rhoades E, Gruber DF, Kao H, Pieribone VA, Regan L. A new bright green‐emitting fluorescent protein – engineered monomeric and dimeric forms. The FEBS Journal 2010, 277: 1967-1978. PMID: 20345907, PMCID: PMC2855763, DOI: 10.1111/j.1742-4658.2010.07618.x.Peer-Reviewed Original Research
2009
Novel Internal Regions of Fluorescent Proteins Undergo Divergent Evolutionary Patterns
Gruber DF, DeSalle R, Lienau EK, Tchernov D, Pieribone VA, Kao HT. Novel Internal Regions of Fluorescent Proteins Undergo Divergent Evolutionary Patterns. Molecular Biology And Evolution 2009, 26: 2841-2848. PMID: 19770223, PMCID: PMC2775108, DOI: 10.1093/molbev/msp194.Peer-Reviewed Original ResearchConceptsFluorescent proteinStrong positive selection pressureDivergent evolutionary pressuresDivergent evolutionary patternsMolecular phylogenetic analysisSuperfamily of proteinsPositive selection pressureCyan fluorescent proteinGreen fluorescent proteinRed fluorescent proteinNatural functionMolecular evolutionPhylogenetic analysisEvolutionary patternsEvolutionary pressureSelection pressureProtein sequencesMarine organismsCompact proteinGeographic originNovel regionBiological researchProteinOrganismsInternal region
2007
Dynamic Regulation of Fluorescent Proteins from a Single Species of Coral
Kao HT, Sturgis S, DeSalle R, Tsai J, Davis D, Gruber DF, Pieribone VA. Dynamic Regulation of Fluorescent Proteins from a Single Species of Coral. Marine Biotechnology 2007, 9: 733-746. PMID: 17955294, DOI: 10.1007/s10126-007-9025-1.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnthozoaBase SequenceDNA PrimersEnvironmentGene Expression ProfilingGene Expression RegulationLuminescent ProteinsMolecular Sequence DataPhylogenyPigmentationReproducibility of ResultsReverse Transcriptase Polymerase Chain ReactionRNA, Ribosomal, 18SSensitivity and SpecificityStatistics as TopicTubulinConceptsFluorescent proteinMontastraea cavernosaSingle speciesBelizean Barrier ReefGroup of proteinsFluorescent protein familyNatural functionProtein familyDynamic regulationBarrier ReefProteinSpeciesOverall expressionCoralsPhysiological conditionsCurrent hypothesesExpressionNatural colorationReefsDistinct spectral classesColorationBetter understandingRegulationTurneffeFamily
2002
A protein kinase A–dependent molecular switch in synapsins regulates neurite outgrowth
Kao HT, Song HJ, Porton B, Ming GL, Hoh J, Abraham M, Czernik AJ, Pieribone VA, Poo MM, Greengard P. A protein kinase A–dependent molecular switch in synapsins regulates neurite outgrowth. Nature Neuroscience 2002, 5: 431-437. PMID: 11976703, DOI: 10.1038/nn840.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodiesBucladesineCells, CulturedConserved SequenceCyclic AMP-Dependent Protein KinasesEmbryo, NonmammalianEnzyme ActivatorsEnzyme InhibitorsGenes, ReporterMicroinjectionsMolecular Sequence DataMutagenesis, Site-DirectedNeuritesNeuronsPhosphorylationRNA, MessengerSequence Homology, Amino AcidSynapsinsXenopus laevisConceptsProtein kinase ANeurite outgrowthCAMP/protein kinase APhospho-specific antibodiesSingle amino acid residueX. laevis embryosAmino acid residuesDependent molecular switchPhosphorylation of synapsinCyclic AMPEmbryonic neuronal culturesNeuronal cell lineProtein kinaseConstitutive phosphorylationKinase AMolecular approachesMolecular switchAcid residuesLaevis embryosPKA inhibitorPotential molecular approachPhosphorylationGrowth-promoting actionCell linesSynapsin
1999
Molecular evolution of the synapsin gene family
Kao H, Porton B, Hilfiker S, Stefani G, Pieribone V, DeSalle R, Greengard P. Molecular evolution of the synapsin gene family. Journal Of Experimental Zoology 1999, 285: 360-377. PMID: 10578110, DOI: 10.1002/(sici)1097-010x(19991215)285:4<360::aid-jez4>3.0.co;2-3.Peer-Reviewed Original ResearchConceptsSynapsin gene familyGene familySynapsin genesGene duplication eventsEvolution of vertebratesVariety of invertebratesSynaptic vesicle proteinsDuplication eventsMolecular evolutionMolecular phylogenyAncestral conditionHigher vertebratesFruit flyRegulation of neurotransmissionVesicle proteinsAdditional speciesProtein sequencesLongfin squidNeuronal phosphoproteinSynapsinVertebratesSpeciesGenesJ. ExpFamilyRegulation of Synaptotagmin I Phosphorylation by Multiple Protein Kinases
Hilfiker S, Pieribone V, Nordstedt C, Greengard P, Czernik A. Regulation of Synaptotagmin I Phosphorylation by Multiple Protein Kinases. Journal Of Neurochemistry 1999, 73: 921-932. PMID: 10461881, DOI: 10.1046/j.1471-4159.1999.0730921.x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCalcium-Binding ProteinsCalcium-Calmodulin-Dependent Protein Kinase Type 2Calcium-Calmodulin-Dependent Protein KinasesCasein Kinase IICell DifferentiationClathrinConserved SequenceHumansIsoenzymesMembrane GlycoproteinsMolecular Sequence DataNerve Tissue ProteinsPC12 CellsPeptide MappingPhosphoamino AcidsPhosphorylationProtein Kinase CProtein KinasesProtein Serine-Threonine KinasesRatsSynaptosomesSynaptotagmin ISynaptotagminsConceptsProtein kinase CSynaptotagmin IProtein kinaseKinase IISynaptic vesicle life cycleCasein kinase IIMultiple protein kinasesClathrin-coated vesiclesDependent protein kinase IICalcium-triggered exocytosisProtein kinase IICytoplasmic domainTryptic phosphopeptidesPhosphorylation stateRat synaptotagmin IKinase CCalcium sensorPhosphorylationKinaseSame residuesSynaptic vesiclesPC12 cellsPhorbol esterThr112I phosphorylation
1998
Regulation of iron metabolism in the sanguivore lamprey Lampetra fluviatilis
Andersen Ø, Pantopoulos K, Kao H, Muckenthaler M, Youson J, Pieribone V. Regulation of iron metabolism in the sanguivore lamprey Lampetra fluviatilis. The FEBS Journal 1998, 254: 223-229. PMID: 9660174, DOI: 10.1046/j.1432-1327.1998.2540223.x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceCloning, MolecularConserved SequenceDNA PrimersDNA, ComplementaryEvolution, MolecularFerritinsHumansIn Vitro TechniquesIronIron Regulatory Protein 1Iron Regulatory Protein 2Iron-Regulatory ProteinsIron-Sulfur ProteinsLampreysMolecular Sequence DataPolymerase Chain ReactionProtein ConformationRatsRNA-Binding ProteinsSequence Homology, Amino AcidSequence Homology, Nucleic AcidConceptsIron-responsive elementFerritin iron-responsive elementLamprey Lampetra fluviatilisSequence identityIRE/IRP regulatory systemEvolution of vertebratesLampetra fluviatilisIron regulatory proteinsNorthern blot analysisVertebrate evolutionMammalian extractsAncient lineageExtant representativesCDNA sequenceH-ferritinFerritin cDNASpecific complexUntranslated regionIRP-1Regulatory systemIRP-2Blot analysisLampreyElectrophoretic mobilityKb
1994
Neuropeptide role of both peptide YY and neuropeptide Y in vertebrates suggested by abundant expression of their mRNAS in a cyclostome brain
Söderberg C, Pieribone VA, Dahlstrand J, Brodin L, Larhammar D. Neuropeptide role of both peptide YY and neuropeptide Y in vertebrates suggested by abundant expression of their mRNAS in a cyclostome brain. Journal Of Neuroscience Research 1994, 37: 633-640. PMID: 8028041, DOI: 10.1002/jnr.490370510.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceBrainDNA, ComplementaryGene Expression RegulationHumansIn Situ HybridizationIntestinal MucosaLampreysMolecular Sequence DataNeuropeptide YOrgan SpecificityPeptide BiosynthesisPeptide YYPeptidesPhylogenyRNA, MessengerSequence HomologySpecies SpecificityVertebrates
1993
Evolutionary conservation of synaptosome-associated protein 25 kDa (SNAP-25) shown by Drosophila and Torpedo cDNA clones.
Risinger C, Blomqvist AG, Lundell I, Lambertsson A, Nässel D, Pieribone VA, Brodin L, Larhammar D. Evolutionary conservation of synaptosome-associated protein 25 kDa (SNAP-25) shown by Drosophila and Torpedo cDNA clones. Journal Of Biological Chemistry 1993, 268: 24408-24414. PMID: 8226991, DOI: 10.1016/s0021-9258(20)80540-7.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceBiological EvolutionBlotting, NorthernConserved SequenceDNA, ComplementaryDrosophila melanogasterDrosophila ProteinsIn Situ HybridizationMembrane ProteinsMolecular Sequence DataNerve Tissue ProteinsSequence Homology, Amino AcidSynaptosomal-Associated Protein 25TorpedoConceptsVesicle dockingCDNA clonesMembrane fusionSNAP-25Basic molecular machineryDisplay sequence similarityMembrane-spanning regionSynaptic vesicle dockingDegree of conservationAmino acid identityRay Torpedo marmorataEvolutionary conservationTorpedo proteinDrosophila melanogasterMolecular machinerySequence similarityMembrane attachmentAcid identityNeuron-specific proteinCysteine residuesDrosophilaSynaptobrevinProtein 25Situ hybridizationProteinDistributions of mRNAs for alpha‐2 adrenergic receptor subtypes in rat brain: An in situ hybridization study
Nicholas A, Pieribone V, Hökfelt T. Distributions of mRNAs for alpha‐2 adrenergic receptor subtypes in rat brain: An in situ hybridization study. The Journal Of Comparative Neurology 1993, 328: 575-594. PMID: 8381444, DOI: 10.1002/cne.903280409.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBrainIn Situ HybridizationMaleMolecular Sequence DataRatsRats, Sprague-DawleyReceptors, Adrenergic, alphaRNA, MessengerTissue DistributionConceptsCentral nervous systemNervous systemCerebral cortexSpinal cordReceptor subtypesRat brainAlpha-2 adrenergic receptor subtypesVentrolateral medullary reticular formationRat central nervous systemAlpha-2 receptor subtypesMRNA labelingAlpha 2 receptorsIntermediolateral cell columnThoracic spinal cordNucleus tractus solitariiReticular thalamic nucleusHypothalamic paraventricular nucleusMedullary reticular formationDorsal root gangliaIslands of CallejaAdrenergic receptor subtypesSelective labeling patternsDeep cerebellar nucleiAlpha 2 probeRat alpha-2A
1992
Differential expression of mRNAs for neuropeptide Y-related peptides in rat nervous tissues: possible evolutionary conservation
Pieribone V, Brodin L, Friberg K, Dahlstrand J, Soderberg C, Larhammar D, Hokfelt T. Differential expression of mRNAs for neuropeptide Y-related peptides in rat nervous tissues: possible evolutionary conservation. Journal Of Neuroscience 1992, 12: 3361-3371. PMID: 1527583, PMCID: PMC6575750, DOI: 10.1523/jneurosci.12-09-03361.1992.Peer-Reviewed Original ResearchConceptsNeuropeptide YPeripheral nerve gangliaRat nervous tissuePopulations of neuronsBrainstem neuronsNPY mRNASympathetic gangliaAdrenal glandCaudal medullaCNS neuronsSpinal cordRostral medullaNerve gangliaRat CNSRat brainVisceral organsMammalian CNSPYY mRNANervous tissuePYY immunoreactivityEndocrine cellsPP mRNAPYYNeuronsMidline regionGalanin message-associated peptide (GMAP)- and galanin-like immunoreactivities: Overlapping and differential distributions in the rat
Hökfelt T, Åman K, Arvidsson U, Bedecs K, Ceccatelli S, Hulting A, Langel U, Meister B, Pieribone V, Bartfai T. Galanin message-associated peptide (GMAP)- and galanin-like immunoreactivities: Overlapping and differential distributions in the rat. Neuroscience Letters 1992, 142: 139-142. PMID: 1280789, DOI: 10.1016/0304-3940(92)90358-e.Peer-Reviewed Original ResearchConceptsGalanin-like immunoreactivityGalanin message-associated peptideCentral nervous systemIslets of LangerhansGalanin messageGalanin-LIMost neuronsGalanin antiserumAnterior lobeNervous systemEndocrine tissuesInsulin cellsIndirect immunofluorescenceImmunoreactivityProlactin cellsRatsNeuronsTissueCellsDifferential distributionPancreasPituitaryPeptidesRetinaBrain