2014
Mechanistic Studies of the Genetically Encoded Fluorescent Protein Voltage Probe ArcLight
Han Z, Jin L, Chen F, Loturco JJ, Cohen LB, Bondar A, Lazar J, Pieribone VA. Mechanistic Studies of the Genetically Encoded Fluorescent Protein Voltage Probe ArcLight. PLOS ONE 2014, 9: e113873. PMID: 25419571, PMCID: PMC4242678, DOI: 10.1371/journal.pone.0113873.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAmino AcidsAnimalsCells, CulturedFluorescenceFluorescent DyesGreen Fluorescent ProteinsHEK293 CellsHumansHydrogen-Ion ConcentrationKineticsLuminescent ProteinsMembrane PotentialsMicroscopy, ConfocalMutation, MissenseNeuronsPatch-Clamp TechniquesPrenylationRatsRecombinant Fusion ProteinsSpectrometry, Fluorescence
2011
Random insertion of split-cans of the fluorescent protein venus into Shaker channels yields voltage sensitive probes with improved membrane localization in mammalian cells
Jin L, Baker B, Mealer R, Cohen L, Pieribone V, Pralle A, Hughes T. Random insertion of split-cans of the fluorescent protein venus into Shaker channels yields voltage sensitive probes with improved membrane localization in mammalian cells. Journal Of Neuroscience Methods 2011, 199: 1-9. PMID: 21497167, PMCID: PMC3281265, DOI: 10.1016/j.jneumeth.2011.03.028.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsCell LineCell Line, TumorCell MembraneCytosolDNA Transposable ElementsFluorescent DyesHumansKidneyLuminescent ProteinsMembrane PotentialsMembrane ProteinsMicroscopy, ConfocalMicroscopy, FluorescenceModels, MolecularMutation, MissenseNeuroblastomaPatch-Clamp TechniquesPeptide FragmentsProtein FoldingProtein MultimerizationProtein Structure, SecondaryProtein Structure, TertiaryRecombinant Fusion ProteinsShaker Superfamily of Potassium ChannelsTransfectionConceptsShaker subunitsYellow fluorescent proteinEndoplasmic reticulumMammalian cellsNon-fluorescent halvesMisfolded monomersPlasma membrane expressionFluorescent protein VenusShaker potassium channelFluorescent protein (FP) voltage sensorsMembrane localizationPlasma membraneFluorescent proteinRandom insertionMembrane expressionSubunitsMembrane potentialIntracellular fluorescencePotassium channelsCellsFluorescent probeΔF/FVoltage sensorTetramerizationProtein
2004
A role for talin in presynaptic function
Morgan JR, Di Paolo G, Werner H, Shchedrina VA, Pypaert M, Pieribone VA, De Camilli P. A role for talin in presynaptic function. Journal Of Cell Biology 2004, 167: 43-50. PMID: 15479735, PMCID: PMC2172527, DOI: 10.1083/jcb.200406020.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsBlotting, WesternCell AdhesionClathrinEndocytosisGlutathione TransferaseHumansLampreysLipid MetabolismMicroscopy, ConfocalMicroscopy, Electron, TransmissionPeptidesPhosphotransferases (Alcohol Group Acceptor)Protein BindingProtein Structure, TertiaryRatsSynapsesTalinTime FactorsConceptsSynaptic vesicle endocytosisClathrin-mediated synaptic vesicle endocytosisClathrin-coated pitsEndocytic defectsMajor phosphatidylinositolActin cytoskeletonVesicle endocytosisActin dynamicsKinase interactionTalinPIP kinasePresynaptic compartmentFunctional linkCell adhesionNeuronal synapsesEndocytosisSynaptic rolePresynaptic functionSynaptic actinActinPhosphatidylinositolSynaptic structureIgammaClathrinCytoskeleton