2003
Colocalization of synapsin and actin during synaptic vesicle recycling
Bloom O, Evergren E, Tomilin N, Kjaerulff O, Löw P, Brodin L, Pieribone VA, Greengard P, Shupliakov O. Colocalization of synapsin and actin during synaptic vesicle recycling. Journal Of Cell Biology 2003, 161: 737-747. PMID: 12756235, PMCID: PMC2199372, DOI: 10.1083/jcb.200212140.Peer-Reviewed Original ResearchConceptsSynaptic activityEndocytic zonesSynaptic vesicle recyclingSynaptic vesicle clustersMature nerve terminalsPool of vesiclesNerve terminalsActive synapsesGiant synapseVesicle recyclingAvailability of vesiclesSynapsin immunoreactivitySynaptic vesiclesVesicle clustersProminent reductionSynapsinSynapses
2002
Impaired recycling of synaptic vesicles after acute perturbation of the presynaptic actin cytoskeleton
Shupliakov O, Bloom O, Gustafsson JS, Kjaerulff O, Löw P, Tomilin N, Pieribone VA, Greengard P, Brodin L. Impaired recycling of synaptic vesicles after acute perturbation of the presynaptic actin cytoskeleton. Proceedings Of The National Academy Of Sciences Of The United States Of America 2002, 99: 14476-14481. PMID: 12381791, PMCID: PMC137908, DOI: 10.1073/pnas.212381799.Peer-Reviewed Original ResearchConceptsSynaptic vesicle clustersVesicle clustersSynaptic vesiclesEndocytic zonesC2 toxinClostridium botulinum C2 toxinClathrin-coated pitsSynaptic vesicle cycleSynaptic vesicle recyclingAccumulation of aggregatesRole of actinLamprey reticulospinal synapseBotulinum C2 toxinActin functionVesicle transportActin cytoskeletonCatalytic subunitVesicle cycleVesicle recyclingPlasma membraneActin filamentsImpaired recyclingReversible anchoringRecycled vesiclesS1 fragment
1999
Inhibition of neurotransmitter release in the lamprey reticulospinal synapse by antibody-mediated disruption of SNAP-25 function
Low P, Norlin T, Risinger C, Larhammar D, Pieribone V, Shupliakov O, Brodin L. Inhibition of neurotransmitter release in the lamprey reticulospinal synapse by antibody-mediated disruption of SNAP-25 function. European Journal Of Cell Biology 1999, 78: 787-793. PMID: 10604655, DOI: 10.1016/s0171-9335(99)80029-6.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, MonoclonalAxonsCell MembraneImmunohistochemistryIn Situ HybridizationLampreysMembrane ProteinsMicroinjectionsMicroscopy, ElectronNerve Tissue ProteinsNeurotransmitter AgentsProtein BindingRecombinant Fusion ProteinsSpinal CordSynapsesSynaptic TransmissionSynaptic VesiclesSynaptosomal-Associated Protein 25ConceptsPlasma membraneSynaptic vesiclesSNAP-25Lamprey giant reticulospinal synapsePresynaptic plasma membraneSynaptic vesicle exocytosisLamprey reticulospinal synapseSNARE protein SNAP-25Protein SNAP-25Synaptic vesicle clustersNeurotransmitter releaseSNARE complexMolecular machineryVesicle exocytosisMembrane fusionVesicle cyclingPresynaptic microinjectionVesicle clustersSyntaxinPresynaptic membraneVesiclesElectron microscopic analysisAntibody-mediated disruptionMembraneMicroscopic analysis
1995
Synaptic Vesicle Depletion in Reticulospinal Axons is Reduced by 5‐hydroxytryptamine: Direct Evidence for Presynaptic Modulation of Glutamatergic Transmission
Shupliakov O, Pieribone VA, Gad H, Brodin L. Synaptic Vesicle Depletion in Reticulospinal Axons is Reduced by 5‐hydroxytryptamine: Direct Evidence for Presynaptic Modulation of Glutamatergic Transmission. European Journal Of Neuroscience 1995, 7: 1111-1116. PMID: 7613617, DOI: 10.1111/j.1460-9568.1995.tb01099.x.Peer-Reviewed Original ResearchConceptsReticulospinal axonsPresynaptic Ca2Glutamatergic synaptic transmissionSynaptic glutamate releaseSynaptic vesiclesIntra-axonal recordingsGiant reticulospinal axonsSynaptic vesicle clustersGlutamatergic transmissionPresynaptic modulationGlutamate releaseSpinal cordPresynaptic elementsSynaptic transmissionAction potential stimulationSynaptic vesicle depletionSame axonVesicle depletionAxonsMarked reductionNumber of vesiclesPotential stimulationSynaptic exocytosisActive zoneCa2