2019
Bright Green Biofluorescence in Sharks Derives from Bromo-Kynurenine Metabolism
Park HB, Lam YC, Gaffney JP, Weaver JC, Krivoshik SR, Hamchand R, Pieribone V, Gruber DF, Crawford JM. Bright Green Biofluorescence in Sharks Derives from Bromo-Kynurenine Metabolism. IScience 2019, 19: 1291-1336. PMID: 31402257, PMCID: PMC6831821, DOI: 10.1016/j.isci.2019.07.019.Peer-Reviewed Original ResearchGreen fluorescent proteinGFP-like proteinsSpecies of sharksAcid-binding proteinFluorescence microscopy studiesFatty acid-binding proteinUndescribed groupSmall molecule metabolitesLuminescent phenotypeFluorescent proteinProteinMicrobial infectionsBiofluorescenceMarine environmentSharksSmall moleculesStructural detailsDiscovery of metabolitesWidespread natureCentral nervous systemNervous systemSpectral characterizationSpeciesMetabolitesDiversity
2014
The Covert World of Fish Biofluorescence: A Phylogenetically Widespread and Phenotypically Variable Phenomenon
Sparks JS, Schelly RC, Smith WL, Davis MP, Tchernov D, Pieribone VA, Gruber DF. The Covert World of Fish Biofluorescence: A Phylogenetically Widespread and Phenotypically Variable Phenomenon. PLOS ONE 2014, 9: e83259. PMID: 24421880, PMCID: PMC3885428, DOI: 10.1371/journal.pone.0083259.Peer-Reviewed Original ResearchConceptsFluorescent proteinCoral reef fishesRay-finned fishesNovel fluorescent proteinsColor vision capabilitiesFish lineagesAnimal treeMarine lineagesPhylogenetic distributionEvolutionary historyBony fishIntraspecific communicationLineagesExperimental biologyPhenotypic variabilityFishProteinBiofluorescenceCurrent understandingSpeciesCnidariansGenusDiscoveryBiologyTrees
2011
Random insertion of split-cans of the fluorescent protein venus into Shaker channels yields voltage sensitive probes with improved membrane localization in mammalian cells
Jin L, Baker B, Mealer R, Cohen L, Pieribone V, Pralle A, Hughes T. Random insertion of split-cans of the fluorescent protein venus into Shaker channels yields voltage sensitive probes with improved membrane localization in mammalian cells. Journal Of Neuroscience Methods 2011, 199: 1-9. PMID: 21497167, PMCID: PMC3281265, DOI: 10.1016/j.jneumeth.2011.03.028.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsCell LineCell Line, TumorCell MembraneCytosolDNA Transposable ElementsFluorescent DyesHumansKidneyLuminescent ProteinsMembrane PotentialsMembrane ProteinsMicroscopy, ConfocalMicroscopy, FluorescenceModels, MolecularMutation, MissenseNeuroblastomaPatch-Clamp TechniquesPeptide FragmentsProtein FoldingProtein MultimerizationProtein Structure, SecondaryProtein Structure, TertiaryRecombinant Fusion ProteinsShaker Superfamily of Potassium ChannelsTransfectionConceptsShaker subunitsYellow fluorescent proteinEndoplasmic reticulumMammalian cellsNon-fluorescent halvesMisfolded monomersPlasma membrane expressionFluorescent protein VenusShaker potassium channelFluorescent protein (FP) voltage sensorsMembrane localizationPlasma membraneFluorescent proteinRandom insertionMembrane expressionSubunitsMembrane potentialIntracellular fluorescencePotassium channelsCellsFluorescent probeΔF/FVoltage sensorTetramerizationProtein
2009
Novel Internal Regions of Fluorescent Proteins Undergo Divergent Evolutionary Patterns
Gruber DF, DeSalle R, Lienau EK, Tchernov D, Pieribone VA, Kao HT. Novel Internal Regions of Fluorescent Proteins Undergo Divergent Evolutionary Patterns. Molecular Biology And Evolution 2009, 26: 2841-2848. PMID: 19770223, PMCID: PMC2775108, DOI: 10.1093/molbev/msp194.Peer-Reviewed Original ResearchConceptsFluorescent proteinStrong positive selection pressureDivergent evolutionary pressuresDivergent evolutionary patternsMolecular phylogenetic analysisSuperfamily of proteinsPositive selection pressureCyan fluorescent proteinGreen fluorescent proteinRed fluorescent proteinNatural functionMolecular evolutionPhylogenetic analysisEvolutionary patternsEvolutionary pressureSelection pressureProtein sequencesMarine organismsCompact proteinGeographic originNovel regionBiological researchProteinOrganismsInternal region
2008
Patterns of fluorescent protein expression in Scleractinian corals.
Gruber DF, Kao HT, Janoschka S, Tsai J, Pieribone VA. Patterns of fluorescent protein expression in Scleractinian corals. Biological Bulletin 2008, 215: 143-54. PMID: 18840775, DOI: 10.2307/25470695.Peer-Reviewed Original ResearchConceptsFluorescent proteinGreat Barrier ReefDistribution of zooxanthellaeFluorescent protein distributionMajority of speciesAustralia's Great Barrier ReefClasses of organismsGreen fluorescent proteinFluorescent protein expressionCoral speciesScleractinian coralsMost speciesProtein distributionBarrier ReefCoralsTargeted expressionProteinSpeciesVariety of familiesProtein expressionBiofluorescenceOrganismsStudy sitesSignificant overlapDistinct patterns
2007
Dynamic Regulation of Fluorescent Proteins from a Single Species of Coral
Kao HT, Sturgis S, DeSalle R, Tsai J, Davis D, Gruber DF, Pieribone VA. Dynamic Regulation of Fluorescent Proteins from a Single Species of Coral. Marine Biotechnology 2007, 9: 733-746. PMID: 17955294, DOI: 10.1007/s10126-007-9025-1.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnthozoaBase SequenceDNA PrimersEnvironmentGene Expression ProfilingGene Expression RegulationLuminescent ProteinsMolecular Sequence DataPhylogenyPigmentationReproducibility of ResultsReverse Transcriptase Polymerase Chain ReactionRNA, Ribosomal, 18SSensitivity and SpecificityStatistics as TopicTubulinConceptsFluorescent proteinMontastraea cavernosaSingle speciesBelizean Barrier ReefGroup of proteinsFluorescent protein familyNatural functionProtein familyDynamic regulationBarrier ReefProteinSpeciesOverall expressionCoralsPhysiological conditionsCurrent hypothesesExpressionNatural colorationReefsDistinct spectral classesColorationBetter understandingRegulationTurneffeFamily
2002
A Genetically Targetable Fluorescent Probe of Channel Gating with Rapid Kinetics
Ataka K, Pieribone VA. A Genetically Targetable Fluorescent Probe of Channel Gating with Rapid Kinetics. Biophysical Journal 2002, 82: 509-516. PMID: 11751337, PMCID: PMC1302490, DOI: 10.1016/s0006-3495(02)75415-5.Peer-Reviewed Original ResearchConceptsGreen fluorescent proteinFluorescent proteinSkeletal muscle voltage-gated sodium channelVoltage-gated sodium channelsActivity reporterIntracellular loopChannel gatingTargetable fluorescent probeExcitable cellsFluorescent activity reportersMembrane potential changesExtended depolarizationSkeletal muscleReporterProteinSodium channelsChannel movementFluorescence signalRapid kineticsFluorescent probeCharge movementFluorescence
1999
Synapsins as regulators of neurotransmitter release
Hilfiker S, Pieribone V, Czernik A, Kao H, Augustine G, Greengard P. Synapsins as regulators of neurotransmitter release. Philosophical Transactions Of The Royal Society B Biological Sciences 1999, 354: 269-279. PMID: 10212475, PMCID: PMC1692497, DOI: 10.1098/rstb.1999.0378.Peer-Reviewed Original ResearchConceptsSynapsin functionSynaptic vesiclesSynaptic vesicle life cycleReserve poolNeuron-specific phosphoproteinNeurotransmitter releaseSelective disruptionVesicle dockingMultigene familyNumerous proteinsCytoskeletal meshworkAbundant proteinsCytoskeletal proteinsMolecular mechanismsProtein componentsSynapsinSynapse formationPresynaptic nerve terminalsVesiclesProteinSecond roleRelease of neurotransmittersLife cycleCurrent understandingSquid giant synapse
1993
Evolutionary conservation of synaptosome-associated protein 25 kDa (SNAP-25) shown by Drosophila and Torpedo cDNA clones.
Risinger C, Blomqvist AG, Lundell I, Lambertsson A, Nässel D, Pieribone VA, Brodin L, Larhammar D. Evolutionary conservation of synaptosome-associated protein 25 kDa (SNAP-25) shown by Drosophila and Torpedo cDNA clones. Journal Of Biological Chemistry 1993, 268: 24408-24414. PMID: 8226991, DOI: 10.1016/s0021-9258(20)80540-7.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceBiological EvolutionBlotting, NorthernConserved SequenceDNA, ComplementaryDrosophila melanogasterDrosophila ProteinsIn Situ HybridizationMembrane ProteinsMolecular Sequence DataNerve Tissue ProteinsSequence Homology, Amino AcidSynaptosomal-Associated Protein 25TorpedoConceptsVesicle dockingCDNA clonesMembrane fusionSNAP-25Basic molecular machineryDisplay sequence similarityMembrane-spanning regionSynaptic vesicle dockingDegree of conservationAmino acid identityRay Torpedo marmorataEvolutionary conservationTorpedo proteinDrosophila melanogasterMolecular machinerySequence similarityMembrane attachmentAcid identityNeuron-specific proteinCysteine residuesDrosophilaSynaptobrevinProtein 25Situ hybridizationProtein