2011
Random insertion of split-cans of the fluorescent protein venus into Shaker channels yields voltage sensitive probes with improved membrane localization in mammalian cells
Jin L, Baker B, Mealer R, Cohen L, Pieribone V, Pralle A, Hughes T. Random insertion of split-cans of the fluorescent protein venus into Shaker channels yields voltage sensitive probes with improved membrane localization in mammalian cells. Journal Of Neuroscience Methods 2011, 199: 1-9. PMID: 21497167, PMCID: PMC3281265, DOI: 10.1016/j.jneumeth.2011.03.028.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsCell LineCell Line, TumorCell MembraneCytosolDNA Transposable ElementsFluorescent DyesHumansKidneyLuminescent ProteinsMembrane PotentialsMembrane ProteinsMicroscopy, ConfocalMicroscopy, FluorescenceModels, MolecularMutation, MissenseNeuroblastomaPatch-Clamp TechniquesPeptide FragmentsProtein FoldingProtein MultimerizationProtein Structure, SecondaryProtein Structure, TertiaryRecombinant Fusion ProteinsShaker Superfamily of Potassium ChannelsTransfectionConceptsShaker subunitsYellow fluorescent proteinEndoplasmic reticulumMammalian cellsNon-fluorescent halvesMisfolded monomersPlasma membrane expressionFluorescent protein VenusShaker potassium channelFluorescent protein (FP) voltage sensorsMembrane localizationPlasma membraneFluorescent proteinRandom insertionMembrane expressionSubunitsMembrane potentialIntracellular fluorescencePotassium channelsCellsFluorescent probeΔF/FVoltage sensorTetramerizationProtein
2002
A Genetically Targetable Fluorescent Probe of Channel Gating with Rapid Kinetics
Ataka K, Pieribone VA. A Genetically Targetable Fluorescent Probe of Channel Gating with Rapid Kinetics. Biophysical Journal 2002, 82: 509-516. PMID: 11751337, PMCID: PMC1302490, DOI: 10.1016/s0006-3495(02)75415-5.Peer-Reviewed Original ResearchConceptsGreen fluorescent proteinFluorescent proteinSkeletal muscle voltage-gated sodium channelVoltage-gated sodium channelsActivity reporterIntracellular loopChannel gatingTargetable fluorescent probeExcitable cellsFluorescent activity reportersMembrane potential changesExtended depolarizationSkeletal muscleReporterProteinSodium channelsChannel movementFluorescence signalRapid kineticsFluorescent probeCharge movementFluorescence