2021
Sex difference in kidney electrolyte transport III: Impact of low K intake on thiazide-sensitive cation excretion in male and female mice
Xu S, Li J, Yang L, Wang CJ, Liu T, Weinstein AM, Palmer LG, Wang T. Sex difference in kidney electrolyte transport III: Impact of low K intake on thiazide-sensitive cation excretion in male and female mice. Pflügers Archiv - European Journal Of Physiology 2021, 473: 1749-1760. PMID: 34455480, PMCID: PMC8528772, DOI: 10.1007/s00424-021-02611-5.Peer-Reviewed Original ResearchConceptsGlomerular filtration rateFemale miceUrine volumeNaCl cotransporterLow K intakeMeasurement of functionSex differencesNatriuretic responseFractional excretionK intakeTransport protein expressionFiltration rateNCC abundanceNCC expressionRenal clearanceDistal nephronLK dietCation excretionPlasma KNCC proteinNKCC2 expressionWestern blottingProtein expressionMiceFENaRestoration of proximal tubule flow-activated transport prevents cyst growth in polycystic kidney disease
Du Z, Tian X, Ma M, Somlo S, Weinstein AM, Wang T. Restoration of proximal tubule flow-activated transport prevents cyst growth in polycystic kidney disease. JCI Insight 2021, 6: e146041. PMID: 33886508, PMCID: PMC8262298, DOI: 10.1172/jci.insight.146041.Peer-Reviewed Original ResearchConceptsGlomerular filtration rateGlomerulotubular balanceRenal cyst formationCyst formationReceptor 1 antagonistPolycystic kidney diseaseKidney weightUntreated miceDA1 antagonistControl miceKidney diseaseFiltration rateFractional reabsorptionCystic indexMouse modelCyst growthConditional KOHCO3- absorptionHeterozygous miceSame antagonistsMicePT transportAntagonistEpithelial ciliaHCO3- transport
2019
Sex difference in kidney electrolyte transport II: impact of K+ intake on thiazide-sensitive cation excretion in male and female mice
Li J, Xu S, Yang L, Yang J, Wang CJ, Weinstein AM, Palmer LG, Wang T. Sex difference in kidney electrolyte transport II: impact of K+ intake on thiazide-sensitive cation excretion in male and female mice. American Journal Of Physiology. Renal Physiology 2019, 317: f967-f977. PMID: 31390232, PMCID: PMC6843050, DOI: 10.1152/ajprenal.00125.2019.Peer-Reviewed Original ResearchConceptsHK dietCation excretionWT animalsFemale animalsMale animalsSex differencesRenal clearance experimentsGlomerular filtration rateR KO miceExchanger isoform 3Distal NaHK intakeWestern blot analysisFractional excretionUrine volumeFiltration rateFemale miceKO miceNHE3 abundanceClearance experimentsNCC abundanceLittle sex differenceKnockout miceENaC expressionExcretion
2017
Urinary bladder hypertrophy characteristic of male ROMK Bartter’s mice does not occur in female mice
Kim JM, Xu S, Guo X, Hu H, Dong K, Wang T. Urinary bladder hypertrophy characteristic of male ROMK Bartter’s mice does not occur in female mice. AJP Regulatory Integrative And Comparative Physiology 2017, 314: r334-r341. PMID: 29092859, PMCID: PMC5899254, DOI: 10.1152/ajpregu.00315.2017.Peer-Reviewed Original ResearchConceptsKO miceBladder hypertrophyBladder weightUrinary bladder hypertrophyRenal outer medullary potassium channelSeverity of hydronephrosisWild-type miceROMK knockout miceBladder capacityDetrusor muscleWT miceUrinary tractBartter's syndromeFemale miceSalt wastingHydronephrosisKnockout miceROMK expressionMiceBladderHypertrophyPotassium channelsMRNA levelsSignificant enlargementSyndromeGender difference in kidney electrolyte transport. I. Role of AT1a receptor in thiazide-sensitive Na+-Cl− cotransporter activity and expression in male and female mice
Li J, Hatano R, Xu S, Wan L, Yang L, Weinstein AM, Palmer L, Wang T. Gender difference in kidney electrolyte transport. I. Role of AT1a receptor in thiazide-sensitive Na+-Cl− cotransporter activity and expression in male and female mice. American Journal Of Physiology. Renal Physiology 2017, 313: f505-f513. PMID: 28566500, PMCID: PMC5582908, DOI: 10.1152/ajprenal.00087.2017.Peer-Reviewed Original ResearchMeSH KeywordsAngiotensin IIAnimalsDiuresisFemaleHydrochlorothiazideKidneyMaleMice, KnockoutNatriuresisPhenotypeProtein Serine-Threonine KinasesReceptor, Angiotensin, Type 1Receptors, DrugSex CharacteristicsSodium Chloride SymportersSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersSolute Carrier Family 12, Member 3ConceptsUrine volumeKO miceCotransporter activityRenal clearance experimentsReceptor knockout miceGlomerular filtration rateBolus intravenous injectionDelivery of NaClNCC protein expressionGender differencesFiltration rateFemale miceAT1A receptorClearance experimentsKO animalsIntravenous injectionNCC expressionDistal nephronFractional NaKnockout miceMale KOProximal tubulesHCTZMiceNHE3 expression
2015
Down-regulation of PRKCB1 expression in Han Chinese patients with subsyndromal symptomatic depression
Guo X, Li Z, Zhang C, Yi Z, Li H, Cao L, Yuan C, Hong W, Wu Z, Peng D, Chen J, Xia W, Zhao G, Wang F, Yu S, Cui D, Xu Y, Golam CM, Smith AK, Wang T, Fang Y. Down-regulation of PRKCB1 expression in Han Chinese patients with subsyndromal symptomatic depression. Journal Of Psychiatric Research 2015, 69: 1-6. PMID: 26343587, DOI: 10.1016/j.jpsychires.2015.07.011.Peer-Reviewed Original ResearchConceptsSubsyndromal symptomatic depressionPeripheral blood mononuclear cellsHan Chinese patientsSSD patientsMRNA expression levelsMDD patientsSymptomatic depressionChinese patientsBlood mononuclear cellsSingle nucleotide polymorphismsExpression levelsMononuclear cellsPatientsMRNA expressionCommon diseaseReal-time quantitative PCR studiesSignificant decreaseSignificant social dysfunctionSocial dysfunctionPCR studiesCandidate genesTranscript networksFunctional annotation toolMultiple single nucleotide polymorphismsQuantitative PCR studies
2012
Regulation of glomerulotubular balance. II. Impact of angiotensin II on flow-dependent transport
Du Z, Wan L, Yan Q, Weinbaum S, Weinstein A, Wang T. Regulation of glomerulotubular balance. II. Impact of angiotensin II on flow-dependent transport. American Journal Of Physiology. Renal Physiology 2012, 303: f1507-f1516. PMID: 22952281, PMCID: PMC3532483, DOI: 10.1152/ajprenal.00277.2012.Peer-Reviewed Original ResearchMeSH KeywordsAngiotensin IIAngiotensin II Type 1 Receptor BlockersAnimalsBicarbonatesBiological TransportEnzyme InhibitorsFemaleHemostasisIn Vitro TechniquesKidney GlomerulusKidney TubulesLosartanMacrolidesMiceMice, KnockoutModels, AnimalProton-Translocating ATPasesReceptor, Angiotensin, Type 1SodiumSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersConceptsNa(+)-H(+) exchanger 3Mouse tubulesImpact of angiotensin IIH-ATPaseMicroperfused in vitroAT(2) receptorsAT(1A) receptorFlow-mediated changesH-ATPase activityParacellular Cl(-Receptor availabilityAngiotensin IIAT(1ARegulate Na(+Increased Na(+Local angiotensinBasolateral transportJ(HCO3Mouse kidneyNet fluidInhibitor bafilomycinProtein levelsMiceReceptorsTubulesGenetic modifiers of hypertension in soluble guanylate cyclase α1–deficient mice
Buys E, Raher M, Kirby A, Mohd S, Baron D, Hayton S, Tainsh L, Sips P, Rauwerdink K, Yan Q, Tainsh R, Shakartzi H, Stevens C, Decaluwé K, da Gloria Rodrigues-Machado M, Malhotra R, Van de Voorde J, Wang T, Brouckaert P, Daly M, Bloch K. Genetic modifiers of hypertension in soluble guanylate cyclase α1–deficient mice. Journal Of Clinical Investigation 2012, 122: 2316-2325. PMID: 22565307, PMCID: PMC3366402, DOI: 10.1172/jci60119.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCyclic GMPEndothelium, VascularFemaleGenetic LinkageGenome, HumanGuanylate CyclaseHumansHypertensionMaleMiceMice, KnockoutQuantitative Trait LociRatsReceptors, Cytoplasmic and NuclearReninRenin-Angiotensin SystemSecond Messenger SystemsSoluble Guanylyl CyclaseSpecies SpecificityVasodilationConceptsRenin-angiotensin-aldosterone systemRenin-angiotensin-aldosterone system inhibitionRelaxation of vascular smooth muscleVascular smooth muscleInducing relaxation of vascular smooth muscleNitric oxideEndothelium-dependent vasorelaxationSubunit of soluble guanylate cyclaseSoluble guanylate cyclaseImproving endothelium-dependent vasorelaxationSmooth muscleQuantitative trait lociArterial pressureMale miceMouse strainsNO/cGMP signalingInduced relaxationAssociated with increased activityHypertensionGuanylate cyclaseBlood flowMiceGenetic modifiersReceptor componentsGenetic factorsRegulation of glomerulotubular balance. I. Impact of dopamine on flow-dependent transport
Du Z, Yan Q, Wan L, Weinbaum S, Weinstein A, Wang T. Regulation of glomerulotubular balance. I. Impact of dopamine on flow-dependent transport. American Journal Of Physiology. Renal Physiology 2012, 303: f386-f395. PMID: 22552936, PMCID: PMC3433864, DOI: 10.1152/ajprenal.00531.2011.Peer-Reviewed Original ResearchMeSH Keywords8-Bromo Cyclic Adenosine MonophosphateAlgorithmsAnimalsBenzazepinesBicarbonatesBiological Transport, ActiveChloridesCyclic AMP-Dependent Protein KinasesDopamineDopamine AntagonistsDopamine D2 Receptor AntagonistsFemaleIsoquinolinesKidney GlomerulusKidney TubulesKidney Tubules, ProximalMacrolidesMiceMicrovilliProton-Translocating ATPasesReceptors, Dopamine D1SodiumSulfonamidesSulpirideConceptsNa/H exchanger 3Impact of dopamineNa(+) transportNa-K-ATPase activityResponse to volume expansionProximal tubules in vitroCl(-) reabsorptionPKA-mediated mechanismMicroperfused in vitroEffects of exogenous dopamineMouse proximal tubuleNa-K-ATPaseProximal tubule reabsorptionStimulation of Cl(-PKA inhibitor H89Luminal flowH-ATPase activityTubules in vitroLuminal flow rateNHE3 activityDA1 receptorsNa/H exchangeTubule reabsorptionProximal tubulesInhibitor H89
2008
Female ROMK null mice manifest more severe Bartter II phenotype on renal function and higher PGE2 production
Yan Q, Yang X, Cantone A, Giebisch G, Hebert S, Wang T. Female ROMK null mice manifest more severe Bartter II phenotype on renal function and higher PGE2 production. AJP Regulatory Integrative And Comparative Physiology 2008, 295: r997-r1004. PMID: 18579648, PMCID: PMC2536865, DOI: 10.1152/ajpregu.00051.2007.Peer-Reviewed Original ResearchConceptsROMK null miceFemale null miceNull miceRenal functionSurvival rateExtent of hydronephrosisDegree of hydronephrosisSeverity of hydronephrosisHigher PGE2 productionHydronephrotic miceLower GFRSyndrome pathophysiologyNg/24 hAcid-base parametersFemale micePGE2 productionHydronephrosisHigher survival rateMiceExcretionSignificant differencesUrinaryMalesSexPossible mechanismMouse model of type II Bartter's syndrome. I. Upregulation of thiazide-sensitive Na-Cl cotransport activity
Cantone A, Yang X, Yan Q, Giebisch G, Hebert SC, Wang T. Mouse model of type II Bartter's syndrome. I. Upregulation of thiazide-sensitive Na-Cl cotransport activity. American Journal Of Physiology. Renal Physiology 2008, 294: f1366-f1372. PMID: 18385266, DOI: 10.1152/ajprenal.00608.2007.Peer-Reviewed Original ResearchMeSH KeywordsAdaptation, PhysiologicalAmilorideAnimalsBartter SyndromeCation Transport ProteinsChloridesDisease Models, AnimalDiureticsEpithelial Sodium ChannelsFemaleFurosemideGlomerular Filtration RateHydrochlorothiazideLoop of HenleMaleMiceMice, Mutant StrainsPotassium Channels, Inwardly RectifyingPregnancySodiumSodium-Potassium-Chloride SymportersSolute Carrier Family 12, Member 1Up-RegulationConceptsThick ascending limbExcretion rateWhole kidney glomerular filtration rateKidney glomerular filtration rateFractional excretion rateFurosemide-induced incrementsGlomerular filtration rateType II Bartter's syndromeHyperprostaglandin E syndromeEffect of furosemideDistal nephron segmentsDistal convoluted tubuleExaggerated natriuresisROMK null miceNa excretionFiltration rateRenal saltAbsolute excretionBartter's syndromeE syndromeMouse modelClearance studiesConvoluted tubulesAscending limbNull mice
2006
Low Na intake suppresses expression of CYP2C23 and arachidonic acid-induced inhibition of ENaC
Sun P, Lin D, Wang T, Babilonia E, Wang Z, Jin Y, Kemp R, Nasjletti A, Wang W. Low Na intake suppresses expression of CYP2C23 and arachidonic acid-induced inhibition of ENaC. American Journal Of Physiology. Renal Physiology 2006, 291: f1192-f1200. PMID: 16849695, DOI: 10.1152/ajprenal.00112.2006.Peer-Reviewed Original ResearchMeSH Keywords8,11,14-Eicosatrienoic AcidAnimalsArachidonic AcidCytochrome P-450 CYP2J2Cytochrome P-450 Enzyme SystemEpithelial Sodium ChannelsFemaleIon Channel GatingKidney Tubules, CollectingMaleMembrane PotentialsPatch-Clamp TechniquesRatsRats, Sprague-DawleySodium, DietarySpecific Pathogen-Free OrganismsConceptsExpression of CYP2C23Low Na intakeCollecting ductsMS-PPOHEpoxyeicosatrienoic acidsDistal nephronArachidonic acidNa intakeInhibition of CYP epoxygenaseNa transportNa absorptionArachidonic acid-induced inhibitionControl Na dietInhibition of ENaC.Na-deficient dietNormal-Na dietActivation of ENaCEpithelial Na channelPatch-clamp studiesHigh-Na dietIncreased Na absorptionAA-induced inhibitionInhibitory effectENaC activityNa restriction
2003
Role of PKC and calcium in modulation of effects of angiotensin II on sodium transport in proximal tubule
Du Z, Ferguson W, Wang T. Role of PKC and calcium in modulation of effects of angiotensin II on sodium transport in proximal tubule. American Journal Of Physiology. Renal Physiology 2003, 284: f688-f692. PMID: 12527554, DOI: 10.1152/ajprenal.00261.2002.Peer-Reviewed Original ResearchConceptsConcentrations of Ang IIAng IIProximal tubulesFluid absorptionTMB-8Effect of luminal ANGLow concentrations of Ang IIEffects of Ang IIEffects of angiotensin IIProximal tubules in vitroProximal tubule transportProximal tubules of ratsInhibit Na(+) transportIntracellular calcium mobilizationAng II concentrationsTubules of ratsCAMP-independent mechanismConcentrations of ANGNa(+) transportTubules in vitroProximal tubular fluidIntracellular Ca(2Intracellular calciumTubule transportLuminal ANG
2002
Formate-stimulated NaCl absorption in the proximal tubule is independent of the pendrin protein
Karniski LP, Wang T, Everett LA, Green ED, Giebisch G, Aronson PS. Formate-stimulated NaCl absorption in the proximal tubule is independent of the pendrin protein. American Journal Of Physiology. Renal Physiology 2002, 283: f952-f956. PMID: 12372770, DOI: 10.1152/ajprenal.00182.2002.Peer-Reviewed Original ResearchConceptsProximal tubulesChloride/formate exchangeNaCl reabsorptionChloride reabsorptionElectroneutral NaCl reabsorptionNaCl absorptionRole of pendrinFormate exchangePds-knockout mouseRecycling of formatePendrin proteinReabsorptionMicePendrinBrush border membrane vesiclesTubulesPresent studyChloride transportApical membraneRole of iNOS and eNOS in modulating proximal tubule transport and acid-base balance
Wang T. Role of iNOS and eNOS in modulating proximal tubule transport and acid-base balance. American Journal Of Physiology. Renal Physiology 2002, 283: f658-f662. PMID: 12217856, DOI: 10.1152/ajprenal.00243.2001.Peer-Reviewed Original ResearchConceptsNeuronal nitric oxide synthaseProximal tubulesEndothelial NOSInducible NOSKnockout miceHCO transportEndothelial NOS knockout miceINOS knockout miceMice lacking neuronal nitric oxide synthaseL-N(6)-(1-iminoethyl) lysineInducible NOS knockoutProximal tubule transportNOS knockout miceKidney proximal tubulesWild-typeIsoforms of NOSWild-type controlsNitric oxide synthaseTubule transportMetabolic acidosisModulate Na(+Acid-base statusJ(HCO3Acid-base balanceFluid absorption