2011
Renal outer medullary potassium channel knockout models reveal thick ascending limb function and dysfunction
Wang T. Renal outer medullary potassium channel knockout models reveal thick ascending limb function and dysfunction. Clinical And Experimental Nephrology 2011, 16: 49-54. PMID: 22038261, DOI: 10.1007/s10157-011-0495-0.Peer-Reviewed Original ResearchConceptsThick ascending limbIon transporter expressionRenal outer medullary potassium channelBartter's syndromeInward rectifier potassium channelPotassium channelsSmall-conductance K channelsROMK null miceMedullary thick ascending limbType II Bartter's syndromeSimilar phenotypeMammalian kidneyApical membraneK channelsROMK knockout miceKnockout modelsChannel activityChannel mutationsRenal functionLimb functionNull micePhysiological conditionsSalt wastingTransporter expressionPathophysiological conditions
2009
Inward rectifier channel, ROMK, is localized to the apical tips of glial‐like cells in mouse taste buds
Dvoryanchikov G, Sinclair M, Perea‐Martinez I, Wang T, Chaudhari N. Inward rectifier channel, ROMK, is localized to the apical tips of glial‐like cells in mouse taste buds. The Journal Of Comparative Neurology 2009, 517: spc1-spc1. DOI: 10.1002/cne.22202.Peer-Reviewed Original ResearchTaste cellsTaste budsTight junctionsHyperpolarized resting membrane potentialInwardly rectifying K channelsMouse taste budsRT-PCRApical tight junctionsMouse taste cellsInward rectifier channelsFungiform taste budsReverse-transcription polymerase chain reactionGlial cell markersQuantitative (q)RT-PCRGlial-like cellsROMK mRNAExtracellular K+Rectifier channelsCell markersPolymerase chain reactionApical tipK channelsTransgenic miceAction potentialsExcess K+Inward rectifier channel, ROMK, is localized to the apical tips of glial‐like cells in mouse taste buds
Dvoryanchikov G, Sinclair M, Perea‐Martinez I, Wang T, Chaudhari N. Inward rectifier channel, ROMK, is localized to the apical tips of glial‐like cells in mouse taste buds. The Journal Of Comparative Neurology 2009, 517: spc1-spc1. DOI: 10.1002/cne.22196.Peer-Reviewed Original ResearchTaste budsTaste cellsHyperpolarized resting membrane potentialTight junctionsInwardly rectifying K channelsMouse taste budsRT-PCRApical tight junctionsMouse taste cellsInward rectifier channelsFungiform taste budsReverse-transcription polymerase chain reactionGlial cell markersRedistribute KQuantitative (q)RT-PCRGlial-like cellsROMK mRNAApical tipExtracellular KRectifier channelsCell markersPolymerase chain reactionK channelsTransgenic miceAction potentialsInward rectifier channel, ROMK, is localized to the apical tips of glial‐like cells in mouse taste buds
Dvoryanchikov G, Sinclair M, Perea‐Martinez I, Wang T, Chaudhari N. Inward rectifier channel, ROMK, is localized to the apical tips of glial‐like cells in mouse taste buds. The Journal Of Comparative Neurology 2009, 517: 1-14. PMID: 19708028, PMCID: PMC3104395, DOI: 10.1002/cne.22152.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsFluorescent Antibody TechniqueGap JunctionsGlutamate DecarboxylaseGreen Fluorescent ProteinsImmunohistochemistryKidneyMiceMice, Inbred C57BLMice, KnockoutMice, TransgenicNeurogliaPhospholipase C betaPotassium Channels, Inwardly RectifyingProtein IsoformsReverse Transcriptase Polymerase Chain ReactionRNA, MessengerTaste BudsTight JunctionsConceptsTaste budsTaste cellsTight junctionsHyperpolarized resting membrane potentialInwardly rectifying K channelsMouse taste budsRT-PCRApical tight junctionsMouse taste cellsInward rectifier channelsFungiform taste budsReverse-transcription polymerase chain reactionGlial cell markersQuantitative (q)RT-PCRGlial-like cellsROMK mRNAApical tipExtracellular K(+Rectifier channelsCell markersPolymerase chain reactionK channelsTransgenic miceAction potentialsBuds
2003
ROMK is required for expression of the 70-pS K channel in the thick ascending limb
Lu M, Wang T, Yan Q, Wang W, Giebisch G, Hebert SC. ROMK is required for expression of the 70-pS K channel in the thick ascending limb. American Journal Of Physiology. Renal Physiology 2003, 286: f490-f495. PMID: 14600033, DOI: 10.1152/ajprenal.00305.2003.Peer-Reviewed Original ResearchConceptsThick ascending limbBartter's syndromeK dietK channelsAscending limbChannel activityApical K channelsFunctional expressionHypokalemic alkalosisTAL cellsNull miceSK activityHeterozygous miceHeterogeneous disorderMicePotassium recyclingROMKFunction mutationsSyndromeCritical subunitApical conductanceSalt absorptionLimbDietExpression
1995
Effects of Glyburide on Renal Tubule Transport and Potassium-Channel Activity
Wang T, Wang W, Klein-Robbenhaar G, Giebisch G. Effects of Glyburide on Renal Tubule Transport and Potassium-Channel Activity. Kidney & Blood Pressure Research 1995, 18: 169-182. PMID: 7481068, DOI: 10.1159/000173914.Peer-Reviewed Original ResearchConceptsK channelsApical membraneInhibition of K secretionApical K channelsRenal tubule transportCortical collecting tubuleK channel activityPatch-clamp techniquePotassium channel activityEffect of glyburideTubule transportK secretionRenal clearanceElectrolyte excretionPotassium channelsTransport of NaAscending limbGlyburideInhibit transportK recyclingKaliuresisHenleMicroperfusionTubulesExcretion