2015
Topographic Mapping of the Synaptic Cleft into Adhesive Nanodomains
de Arce K, Schrod N, Metzbower SWR, Allgeyer E, Kong G, Tang AH, Krupp AJ, Stein V, Liu X, Bewersdorf J, Blanpied TA, Lucić V, Biederer T. Topographic Mapping of the Synaptic Cleft into Adhesive Nanodomains. Neuron 2015, 88: 1165-1172. PMID: 26687224, PMCID: PMC4687029, DOI: 10.1016/j.neuron.2015.11.011.Peer-Reviewed Original ResearchConceptsSynaptic cell adhesion molecule 1Trans-synaptic complexesEphB2 receptor tyrosine kinaseReceptor tyrosine kinasesCryo-ETSynaptic cleftCryoelectron tomographyTyrosine kinaseMolecular insightsSynCAM 1Macromolecular organizationImmunoglobulin proteinCell adhesion molecule-1Immunoelectron microscopyAdhesion molecule-1Super-resolution imagingPostsynaptic densityDistinct density profilesDepression paradigmExcitatory synapsesPostsynaptic areaMolecule-1Cleft edgesSynapsesCleft
2011
Lateral assembly of the immunoglobulin protein SynCAM 1 controls its adhesive function and instructs synapse formation
Fogel AI, Stagi M, Perez de Arce K, Biederer T. Lateral assembly of the immunoglobulin protein SynCAM 1 controls its adhesive function and instructs synapse formation. The EMBO Journal 2011, 30: 4728-4738. PMID: 21926970, PMCID: PMC3243608, DOI: 10.1038/emboj.2011.336.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell AdhesionCell Adhesion Molecule-1Cell Adhesion MoleculesCell Adhesion Molecules, NeuronalCell DifferentiationCells, CulturedChlorocebus aethiopsCoculture TechniquesCOS CellsFluorescence Resonance Energy TransferHEK293 CellsHippocampusHumansImmunoglobulinsImmunohistochemistryMiceNeuritesProtein Structure, QuaternarySynapsesConceptsSynCAM 1Specialized adhesion sitesSynapse formationTrans-synaptic interactionsSynaptic cleftCI assemblyProtein complexesSynaptic cell adhesion molecule SynCAM 1Adhesion sitesSynaptogenic activityAdhesive functionSynapse developmentStructural organizationNovel insightsSynapse inductionLateral assemblyAdhesive capacityAdhesion moleculesSynaptic morphologyAdhesive mechanismsOligomerizationAssemblyAxo-dendritic contactsCleftLater stages
2010
SynCAM 1 Adhesion Dynamically Regulates Synapse Number and Impacts Plasticity and Learning
Robbins EM, Krupp AJ, de Arce K, Ghosh AK, Fogel AI, Boucard A, Südhof TC, Stein V, Biederer T. SynCAM 1 Adhesion Dynamically Regulates Synapse Number and Impacts Plasticity and Learning. Neuron 2010, 68: 894-906. PMID: 21145003, PMCID: PMC3026433, DOI: 10.1016/j.neuron.2010.11.003.Peer-Reviewed Original ResearchConceptsSynapse numberSynCAM 1Excitatory synapse numberLong-term depressionActivity-dependent changesTransgenic brainsExcitatory synapsesMature synapsesTransgenic miceNeuronal connectivityNeuronal circuitsSynapse developmentSpatial learningAdhesion moleculesNeuronal networksSynapsesAdult networksMiceBrainOverexpressionPlasticityExpressionSynaptogenesisN-Glycosylation at the SynCAM (Synaptic Cell Adhesion Molecule) Immunoglobulin Interface Modulates Synaptic Adhesion*
Fogel AI, Li Y, Giza J, Wang Q, Lam TT, Modis Y, Biederer T. N-Glycosylation at the SynCAM (Synaptic Cell Adhesion Molecule) Immunoglobulin Interface Modulates Synaptic Adhesion*. Journal Of Biological Chemistry 2010, 285: 34864-34874. PMID: 20739279, PMCID: PMC2966101, DOI: 10.1074/jbc.m110.120865.Peer-Reviewed Original ResearchConceptsN-glycosylationTrans-synaptic interactionsN-glycansSite-specific N-glycansSynaptic cell adhesion molecule 1Site-specific N-glycosylationTrans-synaptic adhesionPost-translational modificationsSelect adhesion moleculesMutational studiesSynaptic adhesionGlycosylation sitesHeterophilic interactionsIg1 domainSynapse inductionPostsynaptic membraneAdhesion moleculesNeurobiological questionsSynaptic cleftStructural modelingPresynaptic terminalsDifferential mannerSialic acidCell adhesion molecule-1AdhesionSynCAM 1 participates in axo-dendritic contact assembly and shapes neuronal growth cones
Stagi M, Fogel AI, Biederer T. SynCAM 1 participates in axo-dendritic contact assembly and shapes neuronal growth cones. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 7568-7573. PMID: 20368431, PMCID: PMC2867738, DOI: 10.1073/pnas.0911798107.Peer-Reviewed Original Research
2007
SynCAMs Organize Synapses through Heterophilic Adhesion
Fogel AI, Akins MR, Krupp AJ, Stagi M, Stein V, Biederer T. SynCAMs Organize Synapses through Heterophilic Adhesion. Journal Of Neuroscience 2007, 27: 12516-12530. PMID: 18003830, PMCID: PMC6673342, DOI: 10.1523/jneurosci.2739-07.2007.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell AdhesionCell Adhesion MoleculesCell Adhesion Molecules, NeuronalCell DifferentiationCell LineCells, CulturedCoculture TechniquesHippocampusHumansImmunoglobulinsMacromolecular SubstancesMiceNeural PathwaysPresynaptic TerminalsProtein IsoformsRatsRats, Sprague-DawleySynapsesSynaptic MembranesSynaptic TransmissionTumor Suppressor ProteinsConceptsAdhesion complexesDivergent expression profilesImmunoglobulin superfamily memberHeterophilic complexesProtein familyPosttranslational modificationsHeterophilic adhesionSuperfamily membersCell adhesion moleculeSynapse organizationExpression profilesSynapse developmentSynCAM 1Cell junctionsActive presynaptic terminalsPostsynaptic sideMolecular compositionAdhesion moleculesAdhesive patternsProteinSynaptic cleftPresynaptic terminalsComplexesExcitatory neurotransmissionFunctional synapses
2002
SynCAM, a Synaptic Adhesion Molecule That Drives Synapse Assembly
Biederer T, Sara Y, Mozhayeva M, Atasoy D, Liu X, Kavalali ET, Südhof T. SynCAM, a Synaptic Adhesion Molecule That Drives Synapse Assembly. Science 2002, 297: 1525-1531. PMID: 12202822, DOI: 10.1126/science.1072356.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBrainBrain ChemistryCell Adhesion MoleculesCell Adhesion Molecules, NeuronalCell LineCoculture TechniquesExocytosisHumansImmunoglobulinsMolecular Sequence DataNeuronsProsencephalonProtein Structure, TertiaryRatsReceptors, AMPARecombinant Fusion ProteinsSequence Homology, Amino AcidSynapsesSynaptic TransmissionTransfectionTumor Suppressor ProteinsConceptsSynapse assemblyHomophilic cell adhesion moleculeDomain-containing proteinsPDZ domain proteinsNonneuronal cellsAdhesion moleculesSynaptic adhesion moleculesImmunoglobulin domain-containing proteinsGlutamate receptorsCoordinated assemblyCytoplasmic tailCell adhesion moleculeGlutamatergic synaptic transmissionSynapse formationPostsynaptic specializationsPostsynaptic responsesHippocampal neuronsSynaptic transmissionProteinNerve cellsAssemblyCellsExpressionTight attachmentNeurons