2018
Mapping the Proteome of the Synaptic Cleft through Proximity Labeling Reveals New Cleft Proteins
Cijsouw T, Ramsey AM, Lam TT, Carbone BE, Blanpied TA, Biederer T. Mapping the Proteome of the Synaptic Cleft through Proximity Labeling Reveals New Cleft Proteins. Proteomes 2018, 6: 48. PMID: 30487426, PMCID: PMC6313906, DOI: 10.3390/proteomes6040048.Peer-Reviewed Original ResearchProximity labelingLabel-free quantitation mass spectrometryReceptor-type tyrosine-protein phosphatase zetaProximity labeling approachCell-cell contactSynaptic cleftPost-synaptic specializationsProteomic contentSynapse heterogeneitySynCAM 1Confocal microcopySurface proteinsCell surfaceProteomeSuper-resolution imagingCultured cortical neuronsMammalian brainLabeling approachMolecular compositionReporterProteinCortical neuronsFunctional organizationExcitatory synapsesDiverse set
2017
Transcellular Nanoalignment of Synaptic Function
Biederer T, Kaeser PS, Blanpied TA. Transcellular Nanoalignment of Synaptic Function. Neuron 2017, 96: 680-696. PMID: 29096080, PMCID: PMC5777221, DOI: 10.1016/j.neuron.2017.10.006.Peer-Reviewed Original Research
2015
Topographic Mapping of the Synaptic Cleft into Adhesive Nanodomains
de Arce K, Schrod N, Metzbower SWR, Allgeyer E, Kong G, Tang AH, Krupp AJ, Stein V, Liu X, Bewersdorf J, Blanpied TA, Lucić V, Biederer T. Topographic Mapping of the Synaptic Cleft into Adhesive Nanodomains. Neuron 2015, 88: 1165-1172. PMID: 26687224, PMCID: PMC4687029, DOI: 10.1016/j.neuron.2015.11.011.Peer-Reviewed Original ResearchConceptsSynaptic cell adhesion molecule 1Trans-synaptic complexesEphB2 receptor tyrosine kinaseReceptor tyrosine kinasesCryo-ETSynaptic cleftCryoelectron tomographyTyrosine kinaseMolecular insightsSynCAM 1Macromolecular organizationImmunoglobulin proteinCell adhesion molecule-1Immunoelectron microscopyAdhesion molecule-1Super-resolution imagingPostsynaptic densityDistinct density profilesDepression paradigmExcitatory synapsesPostsynaptic areaMolecule-1Cleft edgesSynapsesCleft
2012
The Synaptic Adhesion Molecule SynCAM 1 Contributes to Cocaine Effects on Synapse Structure and Psychostimulant Behavior
Giza JI, Jung Y, Jeffrey RA, Neugebauer NM, Picciotto MR, Biederer T. The Synaptic Adhesion Molecule SynCAM 1 Contributes to Cocaine Effects on Synapse Structure and Psychostimulant Behavior. Neuropsychopharmacology 2012, 38: 628-638. PMID: 23169347, PMCID: PMC3572459, DOI: 10.1038/npp.2012.226.Peer-Reviewed Original ResearchConceptsNucleus accumbensDrugs of abuseSynCAM 1Cocaine effectsSynapse structureMushroom-type spinesExcitatory synapse numberMedium spiny neuronsAddiction-related behaviorsKO miceSpiny neuronsStubby spinesSynapse numberAdhesion molecule proteinsExcitatory synapsesCocaine administrationTrans-synaptic interactionsKnockout micePsychostimulant effectsNAc synapsesStructural remodelingTherapeutic interventionsSynaptic cleftPsychostimulant cocaineSynapse developmentSpecific N‐glycans on SynCAM Ig proteins regulate synaptic adhesion and synapse development
Biederer T. Specific N‐glycans on SynCAM Ig proteins regulate synaptic adhesion and synapse development. The FASEB Journal 2012, 26: 232.2-232.2. DOI: 10.1096/fasebj.26.1_supplement.232.2.Peer-Reviewed Original ResearchTrans-synaptic interactionsSynapse developmentN-glycansSite-specific N-glycosylationSpecialized cell junctionsSpecific N-glycansProtein complexesFirst Ig domainN-glycosylationBinding interfaceSynaptic adhesionIg domainsFunctional analysisSynCAM 1Cell junctionsIg1 domainImmunoglobulin proteinNovel mechanismIg proteinGlycosylationProteinAdhesive interactionsSynCAMSynaptic cleftExcitatory synapses
2011
Lateral assembly of the immunoglobulin protein SynCAM 1 controls its adhesive function and instructs synapse formation
Fogel AI, Stagi M, Perez de Arce K, Biederer T. Lateral assembly of the immunoglobulin protein SynCAM 1 controls its adhesive function and instructs synapse formation. The EMBO Journal 2011, 30: 4728-4738. PMID: 21926970, PMCID: PMC3243608, DOI: 10.1038/emboj.2011.336.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell AdhesionCell Adhesion Molecule-1Cell Adhesion MoleculesCell Adhesion Molecules, NeuronalCell DifferentiationCells, CulturedChlorocebus aethiopsCoculture TechniquesCOS CellsFluorescence Resonance Energy TransferHEK293 CellsHippocampusHumansImmunoglobulinsImmunohistochemistryMiceNeuritesProtein Structure, QuaternarySynapsesConceptsSynCAM 1Specialized adhesion sitesSynapse formationTrans-synaptic interactionsSynaptic cleftCI assemblyProtein complexesSynaptic cell adhesion molecule SynCAM 1Adhesion sitesSynaptogenic activityAdhesive functionSynapse developmentStructural organizationNovel insightsSynapse inductionLateral assemblyAdhesive capacityAdhesion moleculesSynaptic morphologyAdhesive mechanismsOligomerizationAssemblyAxo-dendritic contactsCleftLater stages
2010
N-Glycosylation at the SynCAM (Synaptic Cell Adhesion Molecule) Immunoglobulin Interface Modulates Synaptic Adhesion*
Fogel AI, Li Y, Giza J, Wang Q, Lam TT, Modis Y, Biederer T. N-Glycosylation at the SynCAM (Synaptic Cell Adhesion Molecule) Immunoglobulin Interface Modulates Synaptic Adhesion*. Journal Of Biological Chemistry 2010, 285: 34864-34874. PMID: 20739279, PMCID: PMC2966101, DOI: 10.1074/jbc.m110.120865.Peer-Reviewed Original ResearchConceptsN-glycosylationTrans-synaptic interactionsN-glycansSite-specific N-glycansSynaptic cell adhesion molecule 1Site-specific N-glycosylationTrans-synaptic adhesionPost-translational modificationsSelect adhesion moleculesMutational studiesSynaptic adhesionGlycosylation sitesHeterophilic interactionsIg1 domainSynapse inductionPostsynaptic membraneAdhesion moleculesNeurobiological questionsSynaptic cleftStructural modelingPresynaptic terminalsDifferential mannerSialic acidCell adhesion molecule-1Adhesion
2007
SynCAMs Organize Synapses through Heterophilic Adhesion
Fogel AI, Akins MR, Krupp AJ, Stagi M, Stein V, Biederer T. SynCAMs Organize Synapses through Heterophilic Adhesion. Journal Of Neuroscience 2007, 27: 12516-12530. PMID: 18003830, PMCID: PMC6673342, DOI: 10.1523/jneurosci.2739-07.2007.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell AdhesionCell Adhesion MoleculesCell Adhesion Molecules, NeuronalCell DifferentiationCell LineCells, CulturedCoculture TechniquesHippocampusHumansImmunoglobulinsMacromolecular SubstancesMiceNeural PathwaysPresynaptic TerminalsProtein IsoformsRatsRats, Sprague-DawleySynapsesSynaptic MembranesSynaptic TransmissionTumor Suppressor ProteinsConceptsAdhesion complexesDivergent expression profilesImmunoglobulin superfamily memberHeterophilic complexesProtein familyPosttranslational modificationsHeterophilic adhesionSuperfamily membersCell adhesion moleculeSynapse organizationExpression profilesSynapse developmentSynCAM 1Cell junctionsActive presynaptic terminalsPostsynaptic sideMolecular compositionAdhesion moleculesAdhesive patternsProteinSynaptic cleftPresynaptic terminalsComplexesExcitatory neurotransmissionFunctional synapsesMixed-culture assays for analyzing neuronal synapse formation
Biederer T, Scheiffele P. Mixed-culture assays for analyzing neuronal synapse formation. Nature Protocols 2007, 2: 670-676. PMID: 17406629, DOI: 10.1038/nprot.2007.92.Peer-Reviewed Original ResearchConceptsVertebrate central nervous systemIndividual cell surface proteinsSynaptic adhesion complexesCell biological mechanismsPostsynaptic membrane domainsCell surface proteinsAssembly of synapsesAdhesion complexesMembrane domainsNeuronal synapse formationIndividual proteinsSynaptogenic activityGenetic studiesBidirectional signalingPrimary neuronal culturesSynapse formationSynaptic differentiationMixed culture assayBiological mechanismsSynaptic structureProteinNew insightsSynaptic cleftNeuronal culturesAssay system
2006
SynCAM in Formation and Function of Synaptic Specializations
Biederer T. SynCAM in Formation and Function of Synaptic Specializations. 2006, 125-135. DOI: 10.1007/978-0-387-32562-0_9.Peer-Reviewed Original ResearchInteraction motifsSynCAM 1Short cytosolic tailProtein interaction motifsSingle transmembrane regionIg-like domainsCytosolic tailActin cytoskeletonTransmembrane regionIntracellular sequencesExtracellular sequencesAdaptor moleculeGeneral roleMembrane differentiationSynaptic differentiationCentral nervous systemPostsynaptic membraneAdhesion moleculesProteinSynaptic cleftMotifPresynaptic terminalsDifferentiationSequenceFamily members