2015
A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation
Pirman NL, Barber KW, Aerni HR, Ma NJ, Haimovich AD, Rogulina S, Isaacs FJ, Rinehart J. A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation. Nature Communications 2015, 6: 8130. PMID: 26350500, PMCID: PMC4566969, DOI: 10.1038/ncomms9130.Peer-Reviewed Original ResearchConceptsProtein phosphorylationProtein phosphorylation eventsFull-length proteinNon-phosphorylated formPhosphoserine-containing proteinsPhosphorylation eventsMEK1 kinaseUAG codonKinase activityRecombinant DNADNA templateEscherichia coliE. coliCodonPhosphorylationFunctional informationSerineProteinColiBiochemical investigationsPhosphoproteomeInefficient productionKinasePhosphoserineDNA
2014
Expanded Cellular Amino Acid Pools Containing Phosphoserine, Phosphothreonine, and Phosphotyrosine
Steinfeld JB, Aerni HR, Rogulina S, Liu Y, Rinehart J. Expanded Cellular Amino Acid Pools Containing Phosphoserine, Phosphothreonine, and Phosphotyrosine. ACS Chemical Biology 2014, 9: 1104-1112. PMID: 24646179, PMCID: PMC4027946, DOI: 10.1021/cb5000532.Peer-Reviewed Original ResearchConceptsNonstandard amino acidsAmino acidsGenetic codeOrthogonal aminoacyl-tRNA synthetaseCellular amino acid poolsIntracellular levelsPhosphorylated amino acidsAminoacyl-tRNA synthetaseE. coliLow-phosphate mediumAmino acid poolCotranslational insertionTRNA pairsMetabolic engineeringRecombinant proteinsDeficient cellsStandard amino acidsProtein synthesisWT cellsPhosphoserinePhosphotyrosinePhosphothreonineProteinAcid poolColi