2015
Bioactive poly(ethylene glycol) hydrogels to recapitulate the HSC niche and facilitate HSC expansion in culture
Cuchiara ML, Coşkun S, Banda OA, Horter KL, Hirschi KK, West JL. Bioactive poly(ethylene glycol) hydrogels to recapitulate the HSC niche and facilitate HSC expansion in culture. Biotechnology And Bioengineering 2015, 113: 870-881. PMID: 26497172, DOI: 10.1002/bit.25848.Peer-Reviewed Original ResearchVascular Niche in HSC Development, Maintenance and Regulation
Coşkun S, Hirschi K. Vascular Niche in HSC Development, Maintenance and Regulation. Stem Cell Biology And Regenerative Medicine 2015, 191-219. DOI: 10.1007/978-3-319-21705-5_9.Peer-Reviewed Original ResearchVascular nicheVascular niche cellsExtraembryonic yolk sacStem cellsBlood cell lineagesHSC ontogenyMammalian embryogenesisAorta-gonadHSC developmentHSC maintenanceMesonephros (AGM) regionHematopoietic stem cellsCellular phenotypesNiche cellsMolecular basisNiche compositionCell lineagesMulti-potent cellsNiche typesExtracellular matrixNicheYolk sacVascular cellsUnique microenvironmentFetal liver
2014
Development of the Fetal Bone Marrow Niche and Regulation of HSC Quiescence and Homing Ability by Emerging Osteolineage Cells
Coşkun S, Chao H, Vasavada H, Heydari K, Gonzales N, Zhou X, de Crombrugghe B, Hirschi KK. Development of the Fetal Bone Marrow Niche and Regulation of HSC Quiescence and Homing Ability by Emerging Osteolineage Cells. Cell Reports 2014, 9: 581-590. PMID: 25310984, PMCID: PMC4266564, DOI: 10.1016/j.celrep.2014.09.013.Peer-Reviewed Original ResearchConceptsOsteolineage cellsHematopoietic stem cellsHSC developmentCellular componentsAdult-repopulating hematopoietic stem cellsBone marrow nicheEmbryonic day 16.5HSC quiescenceMultilineage progenitorsSpecialized nichesNiche formationFetal bone marrow cellsHSC activityMarrow nicheMultilineage coloniesStem cellsBone marrow cellsDay 16.5NicheHSC proliferationHoming abilityMarrow cellsCellsBone marrowStromal components
2010
Establishment and regulation of the HSC niche: Roles of osteoblastic and vascular compartments
Coskun S, Hirschi KK. Establishment and regulation of the HSC niche: Roles of osteoblastic and vascular compartments. Birth Defects Research Part C Embryo Today Reviews 2010, 90: 229-242. PMID: 21181885, PMCID: PMC5226239, DOI: 10.1002/bdrc.20194.Peer-Reviewed Original ResearchConceptsHematopoietic stem cellsAdult bone marrow nichesExtraembryonic yolk sacVascular endothelial cellsAdult bone marrowBone marrow nicheMammalian embryogenesisHSC formationBlood cell typesHematopoiesis changeAorta-gonadHSC generationMesonephros (AGM) regionHSC maturationMajor regulatory factorLifelong supplyMajor cellular componentHSC nicheEndothelial cellsMulti-potent cellsRegulatory factorsCellular componentsCell typesMarrow nicheDifferent tissuesMdm2 Is Required for Survival of Hematopoietic Stem Cells/Progenitors via Dampening of ROS-Induced p53 Activity
Abbas HA, Maccio DR, Coskun S, Jackson JG, Hazen AL, Sills TM, You MJ, Hirschi KK, Lozano G. Mdm2 Is Required for Survival of Hematopoietic Stem Cells/Progenitors via Dampening of ROS-Induced p53 Activity. Cell Stem Cell 2010, 7: 606-617. PMID: 21040902, PMCID: PMC3026610, DOI: 10.1016/j.stem.2010.09.013.Peer-Reviewed Original ResearchConceptsReactive oxygen speciesPostnatal day 13Cell cycle arrestAbsence of Mdm2Bone marrowAntioxidant treatmentStem cells/progenitorsDay 13Hematopoietic stem cellsMicePostnatal bone marrowFetal liverHematopoietic failureCycle arrestHematopoietic compartmentP53 levelsHematopoietic cellsP53 activityCell deathStem cellsMDM2Survival