2009
Reduced in vivo phosphodiesterase-4 response to acute noradrenaline challenge in diet-induced obese rats
Greene M, Thackeray JT, Kenk M, Thorn SL, Bevilacqua L, Harper ME, Beanlands RS, DaSilva JN. Reduced in vivo phosphodiesterase-4 response to acute noradrenaline challenge in diet-induced obese rats. Canadian Journal Of Physiology And Pharmacology 2009, 87: 196-202. PMID: 19295660, DOI: 10.1139/y09-001.Peer-Reviewed Original ResearchConceptsDiet-induced obeseDR ratsDiet-induced obese ratsObese animal modelsSympathetic nervous activityPersistence of obesityHigh-fat dietPhosphodiesterase 4 inhibitorWeeks of feedingEnergy expenditure measuresDIO animalsNoradrenaline challengeDesipramine pretreatmentObese ratsCaloric intakeNoradrenaline stimulationNervous activityAnimal modelsPDE4 levelsNormal increaseWeight gainH pretreatmentSkeletal muscleRatsProgressive alteration
2006
In vivo selective binding of (R)-[11C]rolipram to phosphodiesterase-4 provides the basis for studying intracellular cAMP signaling in the myocardium and other peripheral tissues
Kenk M, Greene M, Thackeray J, deKemp RA, Lortie M, Thorn S, Beanlands RS, DaSilva JN. In vivo selective binding of (R)-[11C]rolipram to phosphodiesterase-4 provides the basis for studying intracellular cAMP signaling in the myocardium and other peripheral tissues. Nuclear Medicine And Biology 2006, 34: 71-77. PMID: 17210463, DOI: 10.1016/j.nucmedbio.2006.10.002.Peer-Reviewed Original ResearchMeSH Keywords3',5'-Cyclic-AMP PhosphodiesterasesAnimalsBrainCarbon RadioisotopesCyclic AMPCyclic Nucleotide Phosphodiesterases, Type 1Cyclic Nucleotide Phosphodiesterases, Type 4HeartMaleMetabolic Clearance RateMyocardiumOrgan SpecificityPhosphodiesterase InhibitorsProtein BindingRadionuclide ImagingRadiopharmaceuticalsRatsRats, Sprague-DawleyRolipramSensitivity and SpecificityTissue DistributionConceptsPhosphodiesterase 4BAY 60Ro 20Male Sprague-Dawley ratsIntracellular cAMPSprague-Dawley ratsNeurohormonal modulationPeripheral tissuesAutoradiography studiesAdipose tissuePDE4 levelsTracer uptakeVivo findingsCAMP-mediated signalingBiodistribution studiesPDE4 activityRolipramSkeletal muscleCAMP levelsTracer retentionCardiac regionCilostazolMyocardiumZaprinastTissue