2020
The Role of RNA Epigenetic Modification in Normal and Malignant Hematopoiesis
Vasic R, Gao Y, Liu C, Halene S. The Role of RNA Epigenetic Modification in Normal and Malignant Hematopoiesis. Current Stem Cell Reports 2020, 6: 144-155. PMID: 33777659, PMCID: PMC7992056, DOI: 10.1007/s40778-020-00178-y.Commentaries, Editorials and LettersHematopoietic stem cellsM6A machineryEpigenetic modificationsRNA modificationsStem cellsNormal hematopoietic stem cellsStem cell fate decisionsCell fate decisionsStem cell functionRNA epigenetic modificationRNA m6A modificationRecognition of RNAImmune cell developmentM6A RNA modificationEraser proteinsFate decisionsM6A readersVariety of functionsGene expressionMalignant hematopoiesisImmune signalingImmune system functionCritical regulatorCell developmentM6A modification
2016
Cooperative Activity of GABP with PU.1 or C/EBPε Regulates Lamin B Receptor Gene Expression, Implicating Their Roles in Granulocyte Nuclear Maturation
Malu K, Garhwal R, Pelletier MG, Gotur D, Halene S, Zwerger M, Yang ZF, Rosmarin AG, Gaines P. Cooperative Activity of GABP with PU.1 or C/EBPε Regulates Lamin B Receptor Gene Expression, Implicating Their Roles in Granulocyte Nuclear Maturation. The Journal Of Immunology 2016, 197: 910-922. PMID: 27342846, PMCID: PMC5022553, DOI: 10.4049/jimmunol.1402285.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCCAAT-Enhancer-Binding ProteinsCell DifferentiationCell NucleusChromatin ImmunoprecipitationElectrophoretic Mobility Shift AssayGA-Binding Protein Transcription FactorGene Expression RegulationGranulocytesHEK293 CellsHematopoietic Stem CellsHumansImmunoblottingMiceMice, Inbred C57BLMutagenesis, Site-DirectedProto-Oncogene ProteinsReal-Time Polymerase Chain ReactionReceptors, Cytoplasmic and NuclearSignal TransductionTrans-ActivatorsConceptsLamin B receptorTranscription factorsGene expressionInner nuclear membrane proteinNuclear membrane proteinsFamily transcription factorsNuclear envelope proteinsETS transcription factorsExpression of genesRole of ETSTranscriptional regulatorsTranscriptional activationCombinatorial actionMembrane proteinsLBR geneEts siteEarly myeloid progenitorsCCAAT enhancerGABPSuch cooperative interactionsNeutrophil differentiationGenesMyeloid progenitorsReceptor gene expressionPromoter
2012
MKL1 and MKL2 play redundant and crucial roles in megakaryocyte maturation and platelet formation
Smith EC, Thon JN, Devine MT, Lin S, Schulz VP, Guo Y, Massaro SA, Halene S, Gallagher P, Italiano JE, Krause DS. MKL1 and MKL2 play redundant and crucial roles in megakaryocyte maturation and platelet formation. Blood 2012, 120: 2317-2329. PMID: 22806889, PMCID: PMC3447785, DOI: 10.1182/blood-2012-04-420828.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine DiphosphateAnimalsBleeding TimeBlood PlateletsBone Marrow CellsCells, CulturedCrosses, GeneticCytoplasmCytoskeletonGene Expression ProfilingHematopoiesisMegakaryocytesMiceMice, Inbred C57BLMice, KnockoutOligonucleotide Array Sequence AnalysisPlatelet ActivationThrombocytopeniaTrans-ActivatorsTranscription FactorsConceptsMegakaryocyte maturationPlatelet formationSerum response factorSerum response factor expressionMembrane organizationGene expressionMKL1MKL2Response factorDKO miceKO backgroundMegakaryocyte compartmentMegakaryocytesCritical roleMegakaryocyte ploidyExpressionMaturationKnockout miceFactor expressionCrucial roleHomologuesGenesMiceProlonged bleeding timeRole
2010
Different Roles of G Protein Subunits β1 and β2 in Neutrophil Function Revealed by Gene Expression Silencing in Primary Mouse Neutrophils*
Zhang Y, Tang W, Jones MC, Xu W, Halene S, Wu D. Different Roles of G Protein Subunits β1 and β2 in Neutrophil Function Revealed by Gene Expression Silencing in Primary Mouse Neutrophils*. Journal Of Biological Chemistry 2010, 285: 24805-24814. PMID: 20525682, PMCID: PMC2915716, DOI: 10.1074/jbc.m110.142885.Peer-Reviewed Original ResearchConceptsGene expressionShort hairpin RNAPrimary mouse neutrophilsHairpin RNADirectional cell migrationFluorescent marker proteinsMouse neutrophilsMouse bone marrow cellsHost innate immunityDouble knockdownExcellent systemIngested bacteriaCell migrationMarker proteinsDivergent rolesKnockdownHematopoietic cellsHematopoietic systemBone marrow cellsSubunits β1Retroviral vectorsInnate immunityBacterial phagocytosisRNAExpression
1999
Improved Expression in Hematopoietic and Lymphoid Cells in Mice After Transplantation of Bone Marrow Transduced With a Modified Retroviral Vector
Halene S, Wang L, Cooper R, Bockstoce D, Robbins P, Kohn D. Improved Expression in Hematopoietic and Lymphoid Cells in Mice After Transplantation of Bone Marrow Transduced With a Modified Retroviral Vector. Blood 1999, 94: 3349-3357. PMID: 10552944, PMCID: PMC9071851, DOI: 10.1182/blood.v94.10.3349.422k05_3349_3357.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAnimalsBone Marrow TransplantationFemaleGene DosageGene ExpressionGene Transfer TechniquesGenetic TherapyGenetic VectorsGreen Fluorescent ProteinsHematopoietic Stem CellsLeukemia Virus, MurineLuminescent ProteinsLymphocytesMaleMiceMice, Inbred C57BLPolymerase Chain ReactionRetroviridaeTime FactorsTransduction, GeneticConceptsEnhanced green fluorescent proteinHematopoietic cellsMoMuLV LTRMammalian hematopoietic cellsMurine embryonic stem cellsStem cellsEmbryonic stem cellsRetroviral vectorsGreen fluorescent proteinMoloney murine leukemia virusMouse bone marrow transplant modelReliable gene expressionHematopoietic stem cellsStable gene transferMurine leukemia virusGene expressionMND vectorTransduction efficiencyFluorescent proteinCopy numberGene transferIndividual cellsAmount of proteinVector copy numberBone marrow transplant model