1990
Quantitation of the ras gene product in leukemic blast cells using enzymatic staining.
Gutheil J, Mane S, Bass K, Lee E, Needleman S. Quantitation of the ras gene product in leukemic blast cells using enzymatic staining. BioTechniques 1990, 9: 212-7. PMID: 2205250.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedBlotting, WesternFemaleHumansImmunoenzyme TechniquesLeukemia, Myelogenous, Chronic, BCR-ABL PositiveLeukemia, Myeloid, AcuteMaleMiddle AgedOncogene Protein p21(ras)Precipitin TestsPrecursor Cell Lymphoblastic Leukemia-LymphomaTumor Cells, CulturedConceptsRas gene productGene productsRas speciesRas protein levelsProtein of interestGene product levelsQuantitation of proteinsSpecific ras mutationsUse of electrophoresisProtein levelsRas mutationsProteinP21 expressionHuman malignanciesSpeciesCellsLeukemic cellsMutationsEnzymatic stainingHigh specificityRadioactive reagentsExpressionElectrophoresisAssaysVivo
1989
Infrequent ras activation in chronic myelogenous leukemia (CML): activating 61st codon mutation in the CML-derived cell line, IM-9.
Needleman S, Gutheil J, Kapil V, Mane S. Infrequent ras activation in chronic myelogenous leukemia (CML): activating 61st codon mutation in the CML-derived cell line, IM-9. Leukemia 1989, 3: 827-9. PMID: 2682048.Peer-Reviewed Original ResearchMeSH KeywordsCodonGene Expression RegulationGenes, rasHumansLeukemia, Myelogenous, Chronic, BCR-ABL PositiveMutationProto-Oncogene MasRNA, MessengerTranscriptional ActivationTumor Cells, CulturedConceptsAcute nonlymphoblastic leukemiaChronic myelogenous leukemiaMyelogenous leukemiaCell linesLymphoblastic cell linesRas gene activationN-rasCML-derived cell linesNonlymphoblastic leukemiaHematopoietic progenitor cellsIM-9LeukemiaProgenitor cellsProto-oncogene cNeoplasmsCodon mutationCell DNAInfrequent eventRas genes