2020
Single-cell RNA sequencing of Trypanosoma brucei from tsetse salivary glands unveils metacyclogenesis and identifies potential transmission blocking antigens
Vigneron A, O'Neill MB, Weiss BL, Savage AF, Campbell OC, Kamhawi S, Valenzuela JG, Aksoy S. Single-cell RNA sequencing of Trypanosoma brucei from tsetse salivary glands unveils metacyclogenesis and identifies potential transmission blocking antigens. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 117: 2613-2621. PMID: 31964820, PMCID: PMC7007551, DOI: 10.1073/pnas.1914423117.Peer-Reviewed Original ResearchConceptsSingle-cell RNA sequencingRNA sequencingInfectious metacyclic formsMetacyclic parasitesMammalian host environmentFly salivary glandsMajor cell clustersClustering of cellsTsetse salivary glandsFamily proteinsDevelopmental programMammalian hostsMetacyclic cellsProtein transcriptsTrypanosoma bruceiDevelopmental processesGene expressionAfrican trypanosomesExpression profilesMolecular mechanismsSalivary glandsNew hostSurface localizationTrypanosome transmissionMetacyclogenesis
2001
Tsetse immune responses and trypanosome transmission: Implications for the development of tsetse-based strategies to reduce trypanosomiasis
Hao Z, Kasumba I, Lehane M, Gibson W, Kwon J, Aksoy S. Tsetse immune responses and trypanosome transmission: Implications for the development of tsetse-based strategies to reduce trypanosomiasis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2001, 98: 12648-12653. PMID: 11592981, PMCID: PMC60108, DOI: 10.1073/pnas.221363798.Peer-Reviewed Original ResearchConceptsFat bodyInsect immune systemAntimicrobial peptide genesFat body tissueImmune responsive genesDifferent life stagesTransgenic approachesResponsive genesAntimicrobial genesImmune responseSubtractive cloningPeptide genesAfrican trypanosomesMolecular signalsImmune systemDifferential regulationInfection processLife stagesTrypanosome transmissionParasite transmissionGenesImportant vectorEscherichia coliBiochemical natureDiptericin