2006
Recruitment of Macrophages and Polymorphonuclear Leukocytes in Lyme Carditis
Montgomery RR, Booth CJ, Wang X, Blaho VA, Malawista SE, Brown CR. Recruitment of Macrophages and Polymorphonuclear Leukocytes in Lyme Carditis. Infection And Immunity 2006, 75: 613-620. PMID: 17101663, PMCID: PMC1828503, DOI: 10.1128/iai.00685-06.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBorrelia burgdorferiColony Count, MicrobialCytokinesDisease Models, AnimalDisease SusceptibilityDNA, BacterialHeartHistocytochemistryLyme DiseaseMacrophagesMiceMice, Inbred C3HMice, Inbred C57BLMice, KnockoutMyocarditisMyocardiumNeutrophilsPolymerase Chain ReactionReceptors, CCR2Receptors, ChemokineUrinary BladderConceptsLyme carditisPolymorphonuclear leukocytesC3H micePresence of PMNsB. burgdorferi burdenNeutrophil chemokine receptorOrgan-specific pathogenesisChemokine receptor CCR2B. burgdorferiRecruitment of macrophagesWild-type miceB. burgdorferi infectionAbsence of macrophagesFunction of macrophagesPeak diseaseInfected heartsLyme arthritisSevere arthritisHeart lesionsReceptor CCR2Severe inflammationHistopathologic examinationChemokine receptorsBurgdorferi infectionCarditis
2004
TROSPA, an Ixodes scapularis Receptor for Borrelia burgdorferi
Pal U, Li X, Wang T, Montgomery RR, Ramamoorthi N, deSilva AM, Bao F, Yang X, Pypaert M, Pradhan D, Kantor FS, Telford S, Anderson JF, Fikrig E. TROSPA, an Ixodes scapularis Receptor for Borrelia burgdorferi. Cell 2004, 119: 457-468. PMID: 15537536, DOI: 10.1016/j.cell.2004.10.027.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntibodies, BacterialAntigens, SurfaceBacterial Outer Membrane ProteinsBacterial VaccinesBase SequenceBorrelia burgdorferiCloning, MolecularGene Expression RegulationHost-Parasite InteractionsIntestinesIxodesLipoproteinsMiceMice, Inbred C3HMolecular Sequence DataReceptors, Cell SurfaceRecombinant ProteinsConceptsLyme disease agent Borrelia burgdorferiSurvival of spirochetesTick receptorMammalian hostsRNA interferenceEfficient colonizationTROSPAOuter surface protein ABorrelia burgdorferiPathogen adherencePathogen transmissionProtein AB. burgdorferi outer surface protein AMRNA levelsIxodes scapularisB. burgdorferiColonizationSurface protein AReceptorsArthropodsBurgdorferiMammalsRepressionSpirochetesTicksDisruption of Ixodes scapularis anticoagulation by using RNA interference
Narasimhan S, Montgomery RR, DePonte K, Tschudi C, Marcantonio N, Anderson JF, Sauer JR, Cappello M, Kantor FS, Fikrig E. Disruption of Ixodes scapularis anticoagulation by using RNA interference. Proceedings Of The National Academy Of Sciences Of The United States Of America 2004, 101: 1141-1146. PMID: 14745044, PMCID: PMC337020, DOI: 10.1073/pnas.0307669100.Peer-Reviewed Original ResearchConceptsAnti-factor Xa activityNumerous infectious diseasesIxodes scapularis ticksRNA interferenceMultiple anticoagulantsReduction of mRNAXa activityVaccine candidatesInfectious diseasesTick salivaAnticoagulantsBabesia microtiPhysiologic functionI. scapularis salivaSalivary glandsBorrelia burgdorferiTick feedingScapularis ticksAnticoagulant activityAnaplasma phagocytophilumTick engorgementSuch interventionsEngorgement weightEngorgementKey target
1997
Borrelia burgdorferi strain-specific Osp C-mediated immunity in mice
Bockenstedt LK, Hodzic E, Feng S, Bourrel KW, de Silva A, Montgomery RR, Fikrig E, Radolf JD, Barthold SW. Borrelia burgdorferi strain-specific Osp C-mediated immunity in mice. Infection And Immunity 1997, 65: 4661-4667. PMID: 9353047, PMCID: PMC175668, DOI: 10.1128/iai.65.11.4661-4667.1997.Peer-Reviewed Original ResearchConceptsChallenge infectionC antiserumImmunization studiesStrain-specific immunityAntibody-mediated immunityPassive immunization studiesImmune mouse serumSurface protein AOuter surface protein ADisease regressionInfected miceBorreliacidal activityC antibodySpirochete Borrelia burgdorferiAnimal modelsImmune serumMouse serumInfectionLyme borreliosisMiceHyperimmune serumOsp AImmunofluorescence studiesBorrelia burgdorferiSurface expressionTemporal pattern of Borrelia burgdorferi p21 expression in ticks and the mammalian host.
Das S, Barthold SW, Giles SS, Montgomery RR, Telford SR, Fikrig E. Temporal pattern of Borrelia burgdorferi p21 expression in ticks and the mammalian host. Journal Of Clinical Investigation 1997, 99: 987-995. PMID: 9062357, PMCID: PMC507907, DOI: 10.1172/jci119264.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, BacterialAntigens, BacterialAntigens, SurfaceArthritis, InfectiousBacterial Outer Membrane ProteinsBacterial ProteinsBacterial VaccinesBorrelia burgdorferi GroupElectrophoresis, Gel, Pulsed-FieldEnzyme-Linked Immunosorbent AssayFemaleFlagellinFluorescent Antibody Technique, IndirectGene Expression Regulation, BacterialHumansImmunizationImmunization, PassiveImmunoblottingIxodesLipoproteinsLyme DiseaseMiceMice, Inbred C3HPlasmidsPolymerase Chain ReactionRecombinant ProteinsRNA, MessengerTime FactorsConceptsInfected miceHumoral responseLate-stage Lyme diseaseMarkers of infectionCourse of diseaseMurine Lyme borreliosisB. burgdorferiB. burgdorferi infectionHuman humoral responseIxodes dammini ticksBurgdorferi-infected miceLyme arthritisActive immunizationMammalian hostsPassive transferBurgdorferi infectionC3H miceMurine infectionDay 14P21 antibodyP21 expressionLyme borreliosisLyme diseaseMiceInfection
1996
Direct demonstration of antigenic substitution of Borrelia burgdorferi ex vivo: exploration of the paradox of the early immune response to outer surface proteins A and C in Lyme disease.
Montgomery RR, Malawista SE, Feen KJ, Bockenstedt LK. Direct demonstration of antigenic substitution of Borrelia burgdorferi ex vivo: exploration of the paradox of the early immune response to outer surface proteins A and C in Lyme disease. Journal Of Experimental Medicine 1996, 183: 261-269. PMID: 8551229, PMCID: PMC2192432, DOI: 10.1084/jem.183.1.261.Peer-Reviewed Original ResearchMeSH KeywordsAdaptation, BiologicalAnimalsAntibodies, BacterialAntibody FormationAntigens, BacterialAntigens, SurfaceBacterial Outer Membrane ProteinsBacterial VaccinesBase SequenceFemaleFluorescent Antibody TechniqueGene ExpressionLipoproteinsLyme DiseaseMiceMice, Inbred C3HMolecular Sequence DataPeritoneal CavityPolymerase Chain ReactionRNA, MessengerSpecific Pathogen-Free OrganismsConceptsOsp AA antibodiesImmune responseOuter surface proteinsLyme diseaseWk of infectionProtective immune responseEarly immune responseReverse transcription-polymerase chain reactionStrong humoral responseB. burgdorferi strain N40Transcription-polymerase chain reactionDirect fluorescent stainingHumoral responsePolymerase chain reactionSurface proteinsEarly courseDay 14Etiologic agentDay 30Vaccine designEx vivoIndirect immunofluorescenceInfectionImmune repertoire