2022
Longitudinal serum proteomics analyses identify unique and overlapping host response pathways in Lyme disease and West Nile virus infection
Boada P, Fatou B, Belperron A, Sigdel T, Smolen K, Wurie Z, Levy O, Ronca S, Murray K, Liberto J, Rashmi P, Kerwin M, Montgomery R, Bockenstedt L, Steen H, Sarwal M. Longitudinal serum proteomics analyses identify unique and overlapping host response pathways in Lyme disease and West Nile virus infection. Frontiers In Immunology 2022, 13: 1012824. PMID: 36569838, PMCID: PMC9784464, DOI: 10.3389/fimmu.2022.1012824.Peer-Reviewed Original ResearchConceptsWest Nile virus infectionLyme diseaseVirus infectionWNV infectionSerum proteomeSymptomatic WNV infectionTime of diagnosisHealthy control seraDisseminated Lyme diseaseHost response pathwaysExtracellular bacterial infectionsSerum proteomic analysisIntracellular viral infectionsViral infectionHost responseBacterial infectionsControl seraStudy participantsInfectionDiseaseDisease biomarkersEarly diagnosticsLC/MSMolecular mechanismsRecovery phase
2021
Single cell immunophenotyping of the skin lesion erythema migrans Identifies IgM memory B cells
Jiang R, Meng H, Raddassi K, Fleming I, Hoehn KB, Dardick KR, Belperron AA, Montgomery RR, Shalek AK, Hafler DA, Kleinstein SH, Bockenstedt LK. Single cell immunophenotyping of the skin lesion erythema migrans Identifies IgM memory B cells. JCI Insight 2021, 6: e148035. PMID: 34061047, PMCID: PMC8262471, DOI: 10.1172/jci.insight.148035.Peer-Reviewed Original ResearchConceptsMemory B cellsErythema migransB cellsEM lesionsIgM memory B cellsLyme diseaseB-cell receptor sequencingSkin infection siteCell receptor sequencingEarly Lyme diseaseLocal antigen presentationSkin immune responsesB cell populationsSingle-cell immunophenotypingMHC class II genesUninvolved skinImmune cellsSpirochetal infectionAntigen presentationCell immunophenotypingT cellsImmune responseIsotype usageAntibody productionInitial signs
2006
Recruitment of Macrophages and Polymorphonuclear Leukocytes in Lyme Carditis
Montgomery RR, Booth CJ, Wang X, Blaho VA, Malawista SE, Brown CR. Recruitment of Macrophages and Polymorphonuclear Leukocytes in Lyme Carditis. Infection And Immunity 2006, 75: 613-620. PMID: 17101663, PMCID: PMC1828503, DOI: 10.1128/iai.00685-06.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBorrelia burgdorferiColony Count, MicrobialCytokinesDisease Models, AnimalDisease SusceptibilityDNA, BacterialHeartHistocytochemistryLyme DiseaseMacrophagesMiceMice, Inbred C3HMice, Inbred C57BLMice, KnockoutMyocarditisMyocardiumNeutrophilsPolymerase Chain ReactionReceptors, CCR2Receptors, ChemokineUrinary BladderConceptsLyme carditisPolymorphonuclear leukocytesC3H micePresence of PMNsB. burgdorferi burdenNeutrophil chemokine receptorOrgan-specific pathogenesisChemokine receptor CCR2B. burgdorferiRecruitment of macrophagesWild-type miceB. burgdorferi infectionAbsence of macrophagesFunction of macrophagesPeak diseaseInfected heartsLyme arthritisSevere arthritisHeart lesionsReceptor CCR2Severe inflammationHistopathologic examinationChemokine receptorsBurgdorferi infectionCarditis
2004
Myeloid Differentiation Antigen 88 Deficiency Impairs Pathogen Clearance but Does Not Alter Inflammation in Borrelia burgdorferi-Infected Mice
Liu N, Montgomery RR, Barthold SW, Bockenstedt LK. Myeloid Differentiation Antigen 88 Deficiency Impairs Pathogen Clearance but Does Not Alter Inflammation in Borrelia burgdorferi-Infected Mice. Infection And Immunity 2004, 72: 3195-3203. PMID: 15155621, PMCID: PMC415708, DOI: 10.1128/iai.72.6.3195-3203.2004.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsAntibodies, BacterialAntigens, DifferentiationArthritisBorrelia burgdorferiDNA, BacterialInflammationLyme DiseaseMacrophages, PeritonealMiceMice, Inbred C57BLMice, KnockoutMyeloid Differentiation Factor 88MyocarditisOpsonin ProteinsPhagocytosisReceptors, ImmunologicUrineConceptsToll-like receptor 2Days of infectionPathogen burdenWT miceAcute inflammationB. burgdorferi-specific antibodyPathogen-specific adaptive immunityMyD88-dependent signaling pathwaysTumor necrosis factor alphaBurgdorferi-specific antibodiesImmunoglobulin G1 responsesTLR2-deficient miceInnate immune cellsBorrelia burgdorferiNecrosis factor alphaWild-type miceIgM titersImmune cellsInflammatory responseFactor alphaAdaptive immunitySpirochete Borrelia burgdorferiWT macrophagesReceptor 2Pathogen clearanceTick Saliva Reduces Adherence and Area of Human Neutrophils
Montgomery RR, Lusitani D, de Boisfleury Chevance A, Malawista SE. Tick Saliva Reduces Adherence and Area of Human Neutrophils. Infection And Immunity 2004, 72: 2989-2994. PMID: 15102811, PMCID: PMC387908, DOI: 10.1128/iai.72.5.2989-2994.2004.Peer-Reviewed Original Research
2002
Human phagocytic cells in the early innate immune response to Borrelia burgdorferi
Montgomery RR, Lusitani D, de Boisfleury Chevance A, Malawista SE. Human phagocytic cells in the early innate immune response to Borrelia burgdorferi. The Journal Of Infectious Diseases 2002, 185: 1773-1779. PMID: 12085324, DOI: 10.1086/340826.Peer-Reviewed Original ResearchConceptsPolymorphonuclear leukocytesImmune responseEarly innate immune responseKilling of spirochetesSpecific antibodiesBorrelia burgdorferiSecondary immune responseInnate immune responseInnate immune systemHuman phagocytic cellsSpirochete clearanceMononuclear cellsImmune systemLyme diseasePhagocytic cellsNatural infectionMature macrophagesSpirochetesIntracellular colocalizationAntibodiesBurgdorferiFirst cellsLimited uptakeCellsMonocytes
2001
Inhibition of Borrelia burgdorferi-Tick Interactions In Vivo by Outer Surface Protein A Antibody
Pal U, Montgomery R, Lusitani D, Voet P, Weynants V, Malawista S, Lobet Y, Fikrig E. Inhibition of Borrelia burgdorferi-Tick Interactions In Vivo by Outer Surface Protein A Antibody. The Journal Of Immunology 2001, 166: 7398-7403. PMID: 11390491, DOI: 10.4049/jimmunol.166.12.7398.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnti-Bacterial AgentsAntibodies, BacterialAntibodies, MonoclonalAntigens, SurfaceBacterial Outer Membrane ProteinsBacterial VaccinesBinding Sites, AntibodyBorrelia burgdorferi GroupDigestive SystemEpitopesFemaleImmune SeraInjections, IntraperitonealInjections, SubcutaneousIxodesLipoproteinsLyme DiseaseLyme Disease VaccinesMiceMice, SCIDMutationProtein Structure, TertiaryRecombinant ProteinsConceptsB. burgdorferi sensu strictoBurgdorferi sensu strictoB. burgdorferi N40Treatment of miceOuter Surface ProteinsB. burgdorferi sensu lato genospeciesSurface protein AOuter surface protein ASCID miceMurine modelB. burgdorferi attachmentLyme diseaseMiceB. burgdorferiBorrelia afzeliiB. afzeliiTick gutBorrelia gariniiOspAVivoAdherenceAntiserumPresent studySurface proteinsGutMurine Lyme Disease: No Evidence for Active Immune Down-Regulation in Resolving or Subclinical Infection
Montgomery R, Wang X, Malawista S. Murine Lyme Disease: No Evidence for Active Immune Down-Regulation in Resolving or Subclinical Infection. The Journal Of Infectious Diseases 2001, 183: 1631-1637. PMID: 11343212, DOI: 10.1086/320703.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBorrelia burgdorferi GroupDisease Models, AnimalFemaleFluorescent Antibody TechniqueInterleukin-1Interleukin-12Lyme DiseaseMacrophage ActivationMacrophagesMiceMyocarditisPeritoneumReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSpecific Pathogen-Free OrganismsTumor Necrosis Factor-alphaConceptsActive diseaseAnti-inflammatory cytokine IL-10Proinflammatory cytokines IL-1Levels of proinflammatoryAnti-inflammatory cytokinesCytokine IL-10Double-label immunofluorescent stainingCytokines IL-1Reverse transcription-polymerase chain reaction analysisSemiquantitative reverse transcription-polymerase chain reaction (RT-PCR) analysisTranscription-polymerase chain reaction analysisInfected heartsIL-10IL-12Inflammatory activityCytokine expressionTumor necrosisLocal macrophagesIL-1Peritoneal cavitySubclinical infectionChain reaction analysisLevels of mRNAMacrophage activationImmune system
2000
Attachment of Borrelia burgdorferi within Ixodes scapularis mediated by outer surface protein A
Pal U, de Silva A, Montgomery R, Fish D, Anguita J, Anderson J, Lobet Y, Fikrig E. Attachment of Borrelia burgdorferi within Ixodes scapularis mediated by outer surface protein A. Journal Of Clinical Investigation 2000, 106: 561-569. PMID: 10953031, PMCID: PMC380253, DOI: 10.1172/jci9427.Peer-Reviewed Original ResearchGeographic clustering of an outer surface protein A mutant of Borrelia burgdorferi. Possible implications of multiple variants for Lyme disease persistence
Malawista SE, Montgomery RR, Wang X, Fu LL, Giles SS. Geographic clustering of an outer surface protein A mutant of Borrelia burgdorferi. Possible implications of multiple variants for Lyme disease persistence. Rheumatology 2000, 39: 537-541. PMID: 10852986, DOI: 10.1093/rheumatology/39.5.537.Peer-Reviewed Original ResearchConceptsLyme arthritisIndex patientsDisease persistenceChronic Lyme arthritisJoint fluid samplesSurface protein AOuter surface protein ANovel epitopesImmune systemPatientsOuter surface proteinsWestern blotLyme diseasePatients' jointsMixed infectionsBorrelia burgdorferiFluid samplesSerumArthritisSurface proteinsGeographic clusteringOspAProtein AFusion protein
1997
Temporal pattern of Borrelia burgdorferi p21 expression in ticks and the mammalian host.
Das S, Barthold SW, Giles SS, Montgomery RR, Telford SR, Fikrig E. Temporal pattern of Borrelia burgdorferi p21 expression in ticks and the mammalian host. Journal Of Clinical Investigation 1997, 99: 987-995. PMID: 9062357, PMCID: PMC507907, DOI: 10.1172/jci119264.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, BacterialAntigens, BacterialAntigens, SurfaceArthritis, InfectiousBacterial Outer Membrane ProteinsBacterial ProteinsBacterial VaccinesBorrelia burgdorferi GroupElectrophoresis, Gel, Pulsed-FieldEnzyme-Linked Immunosorbent AssayFemaleFlagellinFluorescent Antibody Technique, IndirectGene Expression Regulation, BacterialHumansImmunizationImmunization, PassiveImmunoblottingIxodesLipoproteinsLyme DiseaseMiceMice, Inbred C3HPlasmidsPolymerase Chain ReactionRecombinant ProteinsRNA, MessengerTime FactorsConceptsInfected miceHumoral responseLate-stage Lyme diseaseMarkers of infectionCourse of diseaseMurine Lyme borreliosisB. burgdorferiB. burgdorferi infectionHuman humoral responseIxodes dammini ticksBurgdorferi-infected miceLyme arthritisActive immunizationMammalian hostsPassive transferBurgdorferi infectionC3H miceMurine infectionDay 14P21 antibodyP21 expressionLyme borreliosisLyme diseaseMiceInfection
1996
Entry of Borrelia burgdorferi into macrophages is end-on and leads to degradation in lysosomes
Montgomery RR, Malawista SE. Entry of Borrelia burgdorferi into macrophages is end-on and leads to degradation in lysosomes. Infection And Immunity 1996, 64: 2867-2872. PMID: 8698527, PMCID: PMC174158, DOI: 10.1128/iai.64.7.2867-2872.1996.Peer-Reviewed Original ResearchDirect demonstration of antigenic substitution of Borrelia burgdorferi ex vivo: exploration of the paradox of the early immune response to outer surface proteins A and C in Lyme disease.
Montgomery RR, Malawista SE, Feen KJ, Bockenstedt LK. Direct demonstration of antigenic substitution of Borrelia burgdorferi ex vivo: exploration of the paradox of the early immune response to outer surface proteins A and C in Lyme disease. Journal Of Experimental Medicine 1996, 183: 261-269. PMID: 8551229, PMCID: PMC2192432, DOI: 10.1084/jem.183.1.261.Peer-Reviewed Original ResearchMeSH KeywordsAdaptation, BiologicalAnimalsAntibodies, BacterialAntibody FormationAntigens, BacterialAntigens, SurfaceBacterial Outer Membrane ProteinsBacterial VaccinesBase SequenceFemaleFluorescent Antibody TechniqueGene ExpressionLipoproteinsLyme DiseaseMiceMice, Inbred C3HMolecular Sequence DataPeritoneal CavityPolymerase Chain ReactionRNA, MessengerSpecific Pathogen-Free OrganismsConceptsOsp AA antibodiesImmune responseOuter surface proteinsLyme diseaseWk of infectionProtective immune responseEarly immune responseReverse transcription-polymerase chain reactionStrong humoral responseB. burgdorferi strain N40Transcription-polymerase chain reactionDirect fluorescent stainingHumoral responsePolymerase chain reactionSurface proteinsEarly courseDay 14Etiologic agentDay 30Vaccine designEx vivoIndirect immunofluorescenceInfectionImmune repertoire
1994
Fc- And Non-Fc-Mediated Phagocytosis Of Borrelia Burgdorferi By Maerophages
Montgomery R, Nathanson M, Malawista S. Fc- And Non-Fc-Mediated Phagocytosis Of Borrelia Burgdorferi By Maerophages. The Journal Of Infectious Diseases 1994, 170: 890-893. PMID: 7930732, DOI: 10.1093/infdis/170.4.890.Peer-Reviewed Original ResearchConceptsConfocal fluorescence microscopyLyme disease spirocheteB. burgdorferiFluorescence microscopyBacterial surface antigensFc receptorsSurface antigenMacrophagesBorrelia burgdorferiSpirochetesBurgdorferiMajor roleSimilar lackComparable efficiencyUptakeColocalizationPhagocytosisInfectionAntigenLocalizationOuter surface proteins E and F of Borrelia burgdorferi, the agent of Lyme disease
Lam TT, Nguyen TP, Montgomery RR, Kantor FS, Fikrig E, Flavell RA. Outer surface proteins E and F of Borrelia burgdorferi, the agent of Lyme disease. Infection And Immunity 1994, 62: 290-298. PMID: 8262642, PMCID: PMC186099, DOI: 10.1128/iai.62.1.290-298.1994.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAntigens, BacterialBacterial Outer Membrane ProteinsBacterial ProteinsBase SequenceBlotting, WesternBorrelia burgdorferi GroupCloning, MolecularCodonFluorescent Antibody TechniqueGenes, BacterialHumansLipoproteinsLyme DiseaseMolecular Sequence DataMolecular WeightOperonRegulatory Sequences, Nucleic AcidRestriction MappingSequence AlignmentSequence Homology, Nucleic AcidSolubilityConceptsOspE genesMolecular massSignal peptidase IIConsensus cleavage sequenceTranscriptional unitsLeader sequenceCommon promoterBp downstreamOuter surface proteinsProtein EStop codonSurface lipoproteinsLabeling showBorrelia burgdorferiGenesHydrophobic domainCleavage sequenceSurface proteinsAmino acidsPeptidase IIProteinOuter surface protein EGel electrophoresisNucleotidesImmunofluorescence studies