2018
Anti-nociceptive effects of bupivacaine-encapsulated PLGA nanoparticles applied to the compressed dorsal root ganglion in mice
Wang T, Hurwitz O, Shimada SG, Tian D, Dai F, Zhou J, Ma C, LaMotte RH. Anti-nociceptive effects of bupivacaine-encapsulated PLGA nanoparticles applied to the compressed dorsal root ganglion in mice. Neuroscience Letters 2018, 668: 154-158. PMID: 29355697, PMCID: PMC5829013, DOI: 10.1016/j.neulet.2018.01.031.Peer-Reviewed Original ResearchConceptsDorsal root gangliaBehavioral hypersensitivityNeuronal hyperexcitabilityRoot gangliaCompressed dorsal root ganglionNormal nociceptive responsesAnti-nociceptive effectsChronic neuropathic painPostoperative pain managementCentral nervous systemIntact DRGsL4 DRGChronic compressionIpsilateral pawNeuropathic painRadicular painHind pawsPain managementIpsilateral hindNociceptive informationNociceptive responsesPain behaviorIntervertebral foramenNociceptive stimuliLocal anesthetics
2017
Cl− channel is required for CXCL10-induced neuronal activation and itch response in a murine model of allergic contact dermatitis
Qu L, Fu K, Shimada SG, LaMotte RH. Cl− channel is required for CXCL10-induced neuronal activation and itch response in a murine model of allergic contact dermatitis. Journal Of Neurophysiology 2017, 118: 619-624. PMID: 28446581, PMCID: PMC5511864, DOI: 10.1152/jn.00187.2017.Peer-Reviewed Original ResearchConceptsAllergic contact dermatitisSquaric acid dibutylesterAllergic itchNeuronal activationContact hypersensitivityContact dermatitisMurine modelItch-related scratching behaviorBehavioral effectsDorsal root ganglion neuronsItch-like behaviorsPrimary sensory neuronsWhole-cell recordingsPromising therapeutic targetPersistent itchItch responseIonic mechanismsGanglion neuronsScratching behaviorChannel blockersCXCL10Sensory neuronsTherapeutic targetChannel inhibitorsCell recordings
2016
Allergic Contact Dermatitis: A Model of Inflammatory Itch and Pain in Human and Mouse
LaMotte RH. Allergic Contact Dermatitis: A Model of Inflammatory Itch and Pain in Human and Mouse. Advances In Experimental Medicine And Biology 2016, 904: 23-32. PMID: 26900060, PMCID: PMC4910628, DOI: 10.1007/978-94-017-7537-3_2.Peer-Reviewed Original ResearchConceptsAllergic contact dermatitisPersistent itchPathogenesis of ACDSquaric acid dibutyl esterPain-like behaviorsSmall-diameter neuronsPeripheral neural mechanismsPossible therapeutic targetInflammatory itchPruritic disordersCutaneous sitesChemokine CXCL10Excitatory responsesLocal inflammationReceptor CXCR3Contact dermatitisUnmyelinated axonsSpontaneous activityTherapeutic targetAnimal modelsPainSubsequent challengeTopical applicationItchMice
2015
Chronic Compression of the Dorsal Root Ganglion Enhances Mechanically Evoked Pain Behavior and the Activity of Cutaneous Nociceptors in Mice
Wang T, Hurwitz O, Shimada SG, Qu L, Fu K, Zhang P, Ma C, LaMotte RH. Chronic Compression of the Dorsal Root Ganglion Enhances Mechanically Evoked Pain Behavior and the Activity of Cutaneous Nociceptors in Mice. PLOS ONE 2015, 10: e0137512. PMID: 26356638, PMCID: PMC4565551, DOI: 10.1371/journal.pone.0137512.Peer-Reviewed Original ResearchConceptsDorsal root gangliaPunctate mechanical stimuliSpontaneous activityChronic compressionCutaneous nociceptorsL3 dorsal root gangliaPost-operative day 2Evoked pain behaviorsPain-like behaviorsVon Frey filamentsDorsum of footUnoperated control miceMechanical stimuliCutaneous C nociceptorsBehavioral hyperalgesiaCCD surgeryIntraforaminal stenosisMechanical allodyniaRadicular painC-nociceptorsDRG neuronsPain behaviorControl miceEnhanced excitabilityControl neuronsCXCR3 chemokine receptor signaling mediates itch in experimental allergic contact dermatitis
Qu L, Fu K, Yang J, Shimada SG, LaMotte RH. CXCR3 chemokine receptor signaling mediates itch in experimental allergic contact dermatitis. Pain 2015, 156: 1737-1746. PMID: 25932692, PMCID: PMC4545682, DOI: 10.1097/j.pain.0000000000000208.Peer-Reviewed Original ResearchConceptsAllergic contact dermatitisPain-like behaviorsContact hypersensitivityAllergic itchChemokine CXCL10Contact dermatitisCutaneous dorsal root ganglion neuronsPathophysiology of ACDExperimental allergic contact dermatitisDorsal root ganglion neuronsSquaric acid dibutylesterDorsal root gangliaSignificant health burdenUpregulation of CXCL10Chemokine receptor signalingCXCR3 mRNAPersistent itchSpontaneous itchInflammatory painCommon symptomsControl miceReceptor CXCR3Skin inflammationGanglion neuronsRoot gangliaPsychophysical Measurements of Itch and Nociceptive Sensations in an Experimental Model of Allergic Contact Dermatitis
Pall PS, Hurwitz OE, King BA, LaMotte RH. Psychophysical Measurements of Itch and Nociceptive Sensations in an Experimental Model of Allergic Contact Dermatitis. Journal Of Pain 2015, 16: 741-749. PMID: 26002605, PMCID: PMC4522332, DOI: 10.1016/j.jpain.2015.04.009.Peer-Reviewed Original ResearchConceptsSquaric acid dibutyl esterAllergic contact dermatitisSpontaneous itchContact dermatitisNociceptive sensationsT cell-mediated inflammationExperimental modelPeripheral neural activityQuality of lifeInflammatory itchBAM8-22Skin inflammationHypersensitivity reactionsPain sensationAllergen resultsIntradermal injectionCommon conditionHealthy volunteersPotential treatmentItchPsychophysical measurementsInflammationVolar forearmDermatitisNeural activity
2014
Enhanced excitability of MRGPRA3- and MRGPRD-positive nociceptors in a model of inflammatory itch and pain
Qu L, Fan N, Ma C, Wang T, Han L, Fu K, Wang Y, Shimada SG, Dong X, LaMotte RH. Enhanced excitability of MRGPRA3- and MRGPRD-positive nociceptors in a model of inflammatory itch and pain. Brain 2014, 137: 1039-1050. PMID: 24549959, PMCID: PMC3959553, DOI: 10.1093/brain/awu007.Peer-Reviewed Original ResearchConceptsContact hypersensitivityPruriceptive neuronsEnhanced excitabilityNervous systemNociceptive dorsal root ganglion (DRG) neuronsSodium currentVehicle-treated control animalsDorsal root ganglion neuronsSquaric acid dibutyl esterItch-like behaviorsPain-related behaviorsResistant sodium currentsAllergic contact dermatitisAction potential activityWhole-cell recordingsInflammatory itchPersistent itchPruritic disordersSpontaneous itchSpontaneous scratchingAcute itchNeuronal hyperexcitabilityNociceptive neuronsPruritic diseasesCommon symptoms
2012
A subpopulation of nociceptors specifically linked to itch
Han L, Ma C, Liu Q, Weng HJ, Cui Y, Tang Z, Kim Y, Nie H, Qu L, Patel KN, Li Z, McNeil B, He S, Guan Y, Xiao B, LaMotte RH, Dong X. A subpopulation of nociceptors specifically linked to itch. Nature Neuroscience 2012, 16: 174-182. PMID: 23263443, PMCID: PMC3557753, DOI: 10.1038/nn.3289.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAnimalsAntirheumatic AgentsCalciumCapsaicinCells, CulturedChloroquineEpidermisGanglia, SpinalGene Expression RegulationGreen Fluorescent ProteinsHistamineHot TemperatureMaleMiceMice, Inbred C57BLMice, TransgenicMotor ActivityNerve FibersNerve Tissue ProteinsNociceptorsPain MeasurementPain ThresholdPatch-Clamp TechniquesPeptide FragmentsPlant LectinsProteinsProto-Oncogene Proteins c-fosPruritusReceptors, BombesinReceptors, G-Protein-CoupledRNA, UntranslatedRotarod Performance TestSensory Receptor CellsSensory System AgentsSpinal CordTRPV Cation Channels
2011
In vivo responses of cutaneous C-mechanosensitive neurons in mouse to punctate chemical stimuli that elicit itch and nociceptive sensations in humans
Ma C, Nie H, Gu Q, Sikand P, LaMotte RH. In vivo responses of cutaneous C-mechanosensitive neurons in mouse to punctate chemical stimuli that elicit itch and nociceptive sensations in humans. Journal Of Neurophysiology 2011, 107: 357-363. PMID: 21994268, PMCID: PMC3349700, DOI: 10.1152/jn.00801.2011.Peer-Reviewed Original ResearchConceptsNociceptive sensationsCowhage spiculesC-nociceptorsNociceptive neuronsReceptive fieldsCutaneous receptive fieldsNoxious mechanical stimuliC-fibre rangePeripheral neural mechanismsExtracellular electrophysiological recordingsAxonal conduction velocityMean discharge rateChemical itchElicit itchChemical stimuliMechanosensitive nociceptorsConduction velocityMechanosensitive neuronsHeat stimuliItchCell bodiesElectrophysiological recordingsVivo responseCapsaicinHistamine
2008
Behavioral differentiation between itch and pain in mouse
Shimada SG, LaMotte RH. Behavioral differentiation between itch and pain in mouse. Pain 2008, 139: 681-687. PMID: 18789837, PMCID: PMC2723192, DOI: 10.1016/j.pain.2008.08.002.Peer-Reviewed Original Research
1999
Mechanical and Thermal Hyperalgesia and Ectopic Neuronal Discharge After Chronic Compression of Dorsal Root Ganglia
Song X, Hu S, Greenquist K, Zhang J, LaMotte R. Mechanical and Thermal Hyperalgesia and Ectopic Neuronal Discharge After Chronic Compression of Dorsal Root Ganglia. Journal Of Neurophysiology 1999, 82: 3347-3358. PMID: 10601466, DOI: 10.1152/jn.1999.82.6.3347.Peer-Reviewed Original ResearchConceptsDorsal root gangliaChronic compressionAcute injuryRoot gangliaPreoperative valuesPostoperative testingFoot withdrawalSpontaneous dischargeIntact sympathetic nervous systemUnmyelinated peripheral nerve fibersEctopic neuronal dischargesEctopic spontaneous dischargesSympathetic nervous systemLow back painPeripheral nerve fibersDorsal root fibersBlood-borne chemicalsExtracellular electrophysiological recordingsActive myelinated fibersCotton wispNonsurgical ratsMechanical hyperalgesiaEctopic dischargesThermal hyperalgesiaBack painAttenuation of experimental pruritus and mechanically evoked dysesthesiae in an area of cutaneous allodynia
Brull S, Atanassoff P, Silverman D, Zhang J, Lamotte R. Attenuation of experimental pruritus and mechanically evoked dysesthesiae in an area of cutaneous allodynia. Somatosensory & Motor Research 1999, 16: 299-303. PMID: 10632027, DOI: 10.1080/08990229970366.Peer-Reviewed Original ResearchConceptsIntradermal injectionTactile allodyniaNociceptive primary afferent neuronsArea of allodyniaAreas of whealCapsaicin-induced allodyniaPrimary afferent neuronsCutaneous hyperalgesiaCutaneous allodyniaAfferent neuronsExperimental armControl armAllodyniaExperimental pruritusDysesthesiaeOpposite forearmVolar surfaceAlloknesisHuman volunteersItchHistamineHyperknesisHyperalgesiaInhibitory processesWhealEnhancement of experimental pruritus and mechanically evoked dysesthesiae with local anesthesia
Atanassoff P, Brull S, Zhang J, Greenquist K, Silverman D, Lamotte R. Enhancement of experimental pruritus and mechanically evoked dysesthesiae with local anesthesia. Somatosensory & Motor Research 1999, 16: 291-298. PMID: 10632026, DOI: 10.1080/08990229970357.Peer-Reviewed Original ResearchConceptsAreas of dysesthesiaDuration of itchHistamine-evoked itchPrimary afferent neuronsAbnormal sensationAfferent neuronsCutaneous areaCentral inhibitionLocal anesthesiaEnhanced painControl armIntradermal injectionLocal anestheticsExperimental pruritusPainItchVolar forearmHistamineMechanical stimulationAfferentsHuman subjectsAnestheticsSkinPresent studySensation
1997
An in vitro study of ectopic discharge generation and adrenergic sensitivity in the intact, nerve-injured rat dorsal root ganglion
Zhang J, Song X, LaMotte R. An in vitro study of ectopic discharge generation and adrenergic sensitivity in the intact, nerve-injured rat dorsal root ganglion. Pain 1997, 72: 51-57. PMID: 9272787, DOI: 10.1016/s0304-3959(97)00013-4.Peer-Reviewed Original ResearchConceptsDorsal root gangliaNerve-injured ratsAdrenergic sensitivitySpontaneous activityUninjured nerveDRG cellsRoot gangliaSciatic nerveL5 dorsal root gangliaIntact dorsal root gangliaSelective alpha2-adrenergic agonistRat dorsal root gangliaAbnormal spontaneous activityInjured DRG neuronsSympathetic nervous systemAlpha2-adrenergic agonistDorsal root fibersIntact blood circulationCutaneous hyperalgesiaSpontaneous painDRG neuronsDorsal rootsSpontaneous dischargeC-fibersPeripheral axonsTensile and Compressive Responses of Nociceptors in Rat Hairy Skin
Khalsa P, Lamotte R, Grigg P. Tensile and Compressive Responses of Nociceptors in Rat Hairy Skin. Journal Of Neurophysiology 1997, 78: 492-505. PMID: 9242296, DOI: 10.1152/jn.1997.78.1.492.Peer-Reviewed Original ResearchConceptsCompressive stressCompressive loadingTensile stressApplied loadFlat indenter tipCompressive responseIndenter tipCompressive loadTensile loadSurface displacementsTip excursionCompressive strainForce controlTensile strainTip geometrySoft substratesLoadingLoadTensile stimulusHard substratesSubstrateStressIndentationCompressionUniform state
1996
Abnormal spontaneous activity and responses to norepinephrine in dissociated dorsal root ganglion cells after chronic nerve constriction
Petersen M, Zhang J, Zhang J, LaMotte R. Abnormal spontaneous activity and responses to norepinephrine in dissociated dorsal root ganglion cells after chronic nerve constriction. Pain 1996, 67: 391-397. PMID: 8951934, DOI: 10.1016/0304-3959(96)03146-6.Peer-Reviewed Original ResearchConceptsDorsal root ganglion cellsUninjured nerveGanglion cellsSpontaneous activityWhole-cell patch-clamp recordingsAbnormal spontaneous activityChronic nerve constrictionPeripheral nerve injuryPrimary sensory neuronsPatch-clamp recordingsMedium-sized cellsNerve constrictionTight ligationNerve injuryDRG cellsSciatic nerveNormal nervesAdult ratsSensory neuronsNerveNorepinephrineLow dosesAction potentialsCultures 11Current clamp
1993
Effect of protons on the inward current evoked by capsaicin in isolated dorsal root ganglion cells
Petersen M, LaMotte R. Effect of protons on the inward current evoked by capsaicin in isolated dorsal root ganglion cells. Pain 1993, 54: 37-42. PMID: 7690929, DOI: 10.1016/0304-3959(93)90097-9.Peer-Reviewed Original ResearchConceptsInward currentsDorsal root ganglion neuronsDorsal root ganglion cellsCapsaicin-evoked currentsWhole-cell voltage-clamp conditionsImportant modulatory roleVoltage-clamp conditionsDRG cellsGanglion neuronsGanglion cellsSensory neuronsModulatory roleCapsaicinProgressive decreaseNeuronsCellsExtracellular medium
1992
Central changes in processing of mechanoreceptive input in capsaicin‐induced secondary hyperalgesia in humans.
Torebjörk H, Lundberg L, LaMotte R. Central changes in processing of mechanoreceptive input in capsaicin‐induced secondary hyperalgesia in humans. The Journal Of Physiology 1992, 448: 765-780. PMID: 1593489, PMCID: PMC1176227, DOI: 10.1113/jphysiol.1992.sp019069.Peer-Reviewed Original ResearchConceptsMechanical hyperalgesiaCapsaicin injectionIntraneural microstimulationMechanoreceptive inputCapsaicin-induced secondary hyperalgesiaCutaneous innervation territoryArea of hyperalgesiaHealthy human subjectsSecondary hyperalgesiaAlgesic substancesLidocaine anesthesiaInnervation territoryIntense painIntradermal injectionUnmyelinated fibersHyperalgesiaMyelinated fibersC-fibersCentral changesPainAfferent signalsImpulse conductionSuprathreshold stimulationSkin areasTactile sensationPain, hyperalgesia and activity in nociceptive C units in humans after intradermal injection of capsaicin.
LaMotte R, Lundberg L, Torebjörk H. Pain, hyperalgesia and activity in nociceptive C units in humans after intradermal injection of capsaicin. The Journal Of Physiology 1992, 448: 749-764. PMID: 1593488, PMCID: PMC1176226, DOI: 10.1113/jphysiol.1992.sp019068.Peer-Reviewed Original ResearchConceptsDuration of painMagnitude of painC-nociceptorsCapsaicin injectionMechanical hyperalgesiaIntradermal injectionTopical applicationReceptive fieldsC-mechanoheat nociceptorsEffects of capsaicinMean discharge rateCutaneous hyperalgesiaHyperalgesic areaUnchanged responsivenessBackground painAlgesic substancesPeroneal nerveNociceptor excitabilityNociceptor responsesPain thresholdMechanical stimuliNociceptor dischargeIntense painConcomitant painAwake humans
1991
Relationships between capsaicin sensitivity of mammalian sensory neurons, cell size and type of voltage gated Ca-currents
Petersen M, LaMotte R. Relationships between capsaicin sensitivity of mammalian sensory neurons, cell size and type of voltage gated Ca-currents. Brain Research 1991, 561: 20-26. PMID: 1665752, DOI: 10.1016/0006-8993(91)90744-g.Peer-Reviewed Original ResearchConceptsCapsaicin sensitivityCultured adult rat dorsal root ganglion neuronsAdult rat dorsal root ganglion neuronsLow-threshold typeRat dorsal root ganglion neuronsWhole-cell patch-clamp techniqueDorsal root ganglion neuronsCultured dorsal root gangliaCapsaicin-sensitive cellsCapsaicin-sensitive populationCross-sectional areaDorsal root gangliaMammalian sensory neuronsPatch-clamp techniqueGanglion neuronsRoot gangliaSensory neuronsCa currentCell populationsActivation thresholdLength of timeLarger cross-sectional areaSectional areaCapsaicinSmall cell population