Specific Induction of Tumor Neovasculature Death by Modified Murine PPE-1 Promoter Armed with HSV-TK
Varda-Bloom N, Hodish I, Shaish A, Greenberger S, Tal R, Feder B, Roitelman J, Breitbart E, Bangio L, Barshack I, Pfeffer R, Harats D. Specific Induction of Tumor Neovasculature Death by Modified Murine PPE-1 Promoter Armed with HSV-TK. Pathobiology 2008, 75: 346-355. PMID: 19096230, DOI: 10.1159/000164219.Peer-Reviewed Original ResearchConceptsHSV-TKSimplex virus thymidine kinaseHSV-TK geneTherapeutic genesVirus thymidine kinaseGene therapyMurine preproendothelin-1 promoterAdenoviral vectorPreproendothelin-1 promoterReporter geneTissue specificityTumor neovasculaturePromoterSpecific inductionGenesHigh expressionTumor angiogenesisMetastasis developmentExpressionNeovasculatureMouse modelKinaseSelectivityEfficiencySpecificityEndothelial-targeted Gene Transfer of Hypoxia-inducible Factor-1α Augments Ischemic Neovascularization Following Systemic Administration
Tal R, Shaish A, Rofe K, Feige E, Varda-Bloom N, Afek A, Barshack I, Bangio L, Hodish I, Greenberger S, Peled M, Breitbart E, Harats D. Endothelial-targeted Gene Transfer of Hypoxia-inducible Factor-1α Augments Ischemic Neovascularization Following Systemic Administration. Molecular Therapy 2008, 16: 1927-1936. PMID: 28189008, DOI: 10.1038/mt.2008.191.Peer-Reviewed Original ResearchTherapeutic efficacyProangiogenic gene therapyHIF-1aMurine preproendothelin-1 promoterTail vein administrationPreproendothelin-1 promoterSystemic side effectsLack of therapeutic efficacySystemic IV administrationHypoxia-inducible factor 1aRoute of administrationIncreased capillary densityMouse hindlimb ischemia modelHindlimb ischemia modelDeliver genesEnhanced blood perfusionGene therapyIntraarterial routeC57BL/6 miceClinical outcomesSystemic toxicityIschemic neovascularizationSide effectsBiodistribution studiesIschemic muscle