2023
Calreticulin Regulates SARS-CoV-2 Spike Protein Turnover and Modulates SARS-CoV-2 Infectivity
Rahimi N, White M, Amraei R, Lotfollahzadeh S, Xia C, Michalak M, Costello C, Mühlberger E. Calreticulin Regulates SARS-CoV-2 Spike Protein Turnover and Modulates SARS-CoV-2 Infectivity. Cells 2023, 12: 2694. PMID: 38067122, PMCID: PMC10705507, DOI: 10.3390/cells12232694.Peer-Reviewed Original ResearchConceptsSARS-CoV-2 infectivityCardiovascular complicationsS-RBDSARS-CoV-2 infectionEndothelial cellsMajor clinical hallmarksSpike proteinCOVID-19 patientsCoronavirus disease 2019SARS-CoV-2 spike proteinSARS-CoV-2S proteinProteasomal inhibitor bortezomibHuman endothelial cellsShRNA-mediated knockdownCalcium hemostasisClinical hallmarkDisease 2019Inhibitor bortezomibCalcium homeostasisAcidification of lysosomesRole of calreticulinTreatment of cellsProtein levelsComplications
2021
NEDD4 regulates ubiquitination and stability of the cell adhesion molecule IGPR-1 via lysosomal pathway
Sun L, Amraei R, Rahimi N. NEDD4 regulates ubiquitination and stability of the cell adhesion molecule IGPR-1 via lysosomal pathway. Journal Of Biomedical Science 2021, 28: 35. PMID: 33962630, PMCID: PMC8103646, DOI: 10.1186/s12929-021-00731-9.Peer-Reviewed Original ResearchConceptsCell adhesion molecule IGPR-1IGPR-1Lysosomal-dependent degradationUbiquitin E3Vivo co-immunoprecipitation assaysWild-type Nedd4Knockdown of NEDD4Polyproline-rich motifCritical cellular processesCell-cell adhesionCo-immunoprecipitation assaysTreatment of cellsCell surface levelsHEK-293 cellsA375 melanoma cellsWW domainsCellular processesRich motifLysosomal pathwayC-terminusNedd4Key regulatorLysosomal inhibitorsMolecular mechanismsMelanoma cell lines