2020
Comparison of Chlamydia outer membrane complex to recombinant outer membrane proteins as vaccine
Yu H, Karunakaran KP, Jiang X, Chan Q, Rose C, Foster LJ, Johnson RM, Brunham RC. Comparison of Chlamydia outer membrane complex to recombinant outer membrane proteins as vaccine. Vaccine 2020, 38: 3280-3291. PMID: 32151463, DOI: 10.1016/j.vaccine.2020.02.059.Peer-Reviewed Original ResearchConceptsChlamydial outer membrane complexRecombinant outer membrane proteinsImmune responseT-cell vaccine candidatesElementary bodiesReactive immune responsesC. muridarum elementary bodiesGenital infectionMajor outer membrane proteinTubal pathologyC. trachomatis serovarsVaccine efficacyOuter membrane proteinsSuperior efficacyVaccine candidatesBroad immunogenicityC. trachomatisVaccineAntibodiesSuperior protectionMultiple outer membrane proteinsOuter membrane complexPathologyEfficacyPolymorphic membrane proteins
2019
Discordance in the Epithelial Cell-Dendritic Cell Major Histocompatibility Complex Class II Immunoproteome: Implications for Chlamydia Vaccine Development
Karunakaran KP, Yu H, Jiang X, Chan QWT, Foster LJ, Johnson RM, Brunham RC. Discordance in the Epithelial Cell-Dendritic Cell Major Histocompatibility Complex Class II Immunoproteome: Implications for Chlamydia Vaccine Development. The Journal Of Infectious Diseases 2019, 221: 841-850. PMID: 31599954, PMCID: PMC7457330, DOI: 10.1093/infdis/jiz522.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, BacterialBacterial VaccinesCD4-Positive T-LymphocytesCell LineChlamydia InfectionsChlamydia muridarumChlamydia trachomatisDendritic CellsEpithelial CellsEpitopes, T-LymphocyteFemaleHeLa CellsHistocompatibility Antigens Class IHistocompatibility Antigens Class IIHost-Pathogen InteractionsHumansMiceMice, Inbred C57BLPeptidesConceptsCD4 T cellsDendritic cellsT cellsEpithelial cellsProtective immunityEffector phaseClass IChlamydia-specific CD4 T cellsPathogen-specific T cellsClass IIMajor histocompatibility complex (MHC) class II moleculesChlamydia vaccine developmentClearance of ChlamydiaClass II epitopesClass II moleculesMHC class IMucosal epithelial cellsInfected epithelial cellsImmune miceIntracellular bacterial pathogenChlamydia vaccineC trachomatisEpithelial cell linePresent epitopesChlamydia trachomatis
2016
Tissue-Resident T Cells as the Central Paradigm of Chlamydia Immunity
Johnson RM, Brunham RC. Tissue-Resident T Cells as the Central Paradigm of Chlamydia Immunity. Infection And Immunity 2016, 84: 868-873. PMID: 26787715, PMCID: PMC4807466, DOI: 10.1128/iai.01378-15.Peer-Reviewed Original ResearchConceptsT cellsTissue-resident memory T cellsTissue-resident T cellsMemory T cellsChlamydia immunityCytokine polarizationTrachoma vaccineChlamydia vaccineVaccine efficacyPathogenesis dataPathogenesis investigationPreclinical pipelineImmunity studiesHuman subjectsVaccineImmune networkCellsMiceImmunity
2012
PmpG303-311, a Protective Vaccine Epitope That Elicits Persistent Cellular Immune Responses in Chlamydia muridarum-Immune Mice
Johnson RM, Yu H, Kerr MS, Slaven JE, Karunakaran KP, Brunham RC. PmpG303-311, a Protective Vaccine Epitope That Elicits Persistent Cellular Immune Responses in Chlamydia muridarum-Immune Mice. Infection And Immunity 2012, 80: 2204-2211. PMID: 22431650, PMCID: PMC3370596, DOI: 10.1128/iai.06339-11.Peer-Reviewed Original ResearchConceptsGenital tract infectionAntigen-presenting cellsT cell clonesTract infectionsMonths postinfectionImmune responseChlamydia muridarum Genital Tract InfectionProtective CD4 T cell responsesVaccine developmentPrimary genital tract infectionTh1 T cell clonesCD4 T cell responsesCD4 T-cell clonesSplenic antigen-presenting cellsAntigen-presenting cell populationsCell clonesCD4 T cellsT cell responsesCellular immune responsesProtective immune responsePromising vaccine candidateImmune C57BL/6 miceInfected epithelial cellsIrradiated splenocytesUrogenital chlamydia