2022
Combining Cellular Immunology With RNAseq to Identify Novel Chlamydia T-Cell Subset Signatures
Johnson RM, Asashima H, Mohanty S, Shaw AC. Combining Cellular Immunology With RNAseq to Identify Novel Chlamydia T-Cell Subset Signatures. The Journal Of Infectious Diseases 2022, 225: 2033-2042. PMID: 35172331, PMCID: PMC9159333, DOI: 10.1093/infdis/jiac051.Peer-Reviewed Original ResearchConceptsProtective T cell clonesAntibacterial effector mechanismsT cells residentB cell helpT cell clonesCytokine polarizationImmune miceIL-10Protective immunityVaccine trialsIL-13Surrogate biomarkerEffector mechanismsGenital tractT cellsVaccine candidatesChlamydia trachomatisCells residentHelper functionCellular immunologyMouse studiesHuman investigationsReproductive tractGranzyme A.Investigational data
2019
Discordance in the Epithelial Cell-Dendritic Cell Major Histocompatibility Complex Class II Immunoproteome: Implications for Chlamydia Vaccine Development
Karunakaran KP, Yu H, Jiang X, Chan QWT, Foster LJ, Johnson RM, Brunham RC. Discordance in the Epithelial Cell-Dendritic Cell Major Histocompatibility Complex Class II Immunoproteome: Implications for Chlamydia Vaccine Development. The Journal Of Infectious Diseases 2019, 221: 841-850. PMID: 31599954, PMCID: PMC7457330, DOI: 10.1093/infdis/jiz522.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, BacterialBacterial VaccinesCD4-Positive T-LymphocytesCell LineChlamydia InfectionsChlamydia muridarumChlamydia trachomatisDendritic CellsEpithelial CellsEpitopes, T-LymphocyteFemaleHeLa CellsHistocompatibility Antigens Class IHistocompatibility Antigens Class IIHost-Pathogen InteractionsHumansMiceMice, Inbred C57BLPeptidesConceptsCD4 T cellsDendritic cellsT cellsEpithelial cellsProtective immunityEffector phaseClass IChlamydia-specific CD4 T cellsPathogen-specific T cellsClass IIMajor histocompatibility complex (MHC) class II moleculesChlamydia vaccine developmentClearance of ChlamydiaClass II epitopesClass II moleculesMHC class IMucosal epithelial cellsInfected epithelial cellsImmune miceIntracellular bacterial pathogenChlamydia vaccineC trachomatisEpithelial cell linePresent epitopesChlamydia trachomatis
2018
B Cell Presentation of Chlamydia Antigen Selects Out Protective CD4γ13 T Cells: Implications for Genital Tract Tissue-Resident Memory Lymphocyte Clusters
Johnson RM, Yu H, Strank N, Karunakaran K, Zhu Y, Brunham RC. B Cell Presentation of Chlamydia Antigen Selects Out Protective CD4γ13 T Cells: Implications for Genital Tract Tissue-Resident Memory Lymphocyte Clusters. Infection And Immunity 2018, 86: 10.1128/iai.00614-17. PMID: 29158429, PMCID: PMC5778355, DOI: 10.1128/iai.00614-17.Peer-Reviewed Original ResearchConceptsMemory lymphocyte clustersPeripheral blood mononuclear cellsT cell subsetsAntigen-presenting cellsT cell clonesB cell populationsT cellsLymphocyte clustersCell subsetsB cellsTissue-resident memory T cellsCD4 T-cell clonesCD4 T cell subsetsHuman peripheral blood mononuclear cellsCell clonesMemory T cellsBlood mononuclear cellsIL-13 responsesImmune B cellsB cell presentationCell populationsAdoptive transferImmune miceIntracellular bacterial pathogenChlamydia antigen