2018
Distinct macrophage populations direct inflammatory versus physiological changes in adipose tissue
Hill DA, Lim HW, Kim YH, Ho WY, Foong YH, Nelson VL, Nguyen HCB, Chegireddy K, Kim J, Habertheuer A, Vallabhajosyula P, Kambayashi T, Won KJ, Lazar MA. Distinct macrophage populations direct inflammatory versus physiological changes in adipose tissue. Proceedings Of The National Academy Of Sciences Of The United States Of America 2018, 115: e5096-e5105. PMID: 29760084, PMCID: PMC5984532, DOI: 10.1073/pnas.1802611115.Peer-Reviewed Original ResearchConceptsMultiple distinct populationsUnique tissue distributionTissue macrophage subsetsChromatin landscapeDistinct macrophage populationsTranscriptional mechanismsGene programObese fat tissueGene expressionDistinct populationsFunctional differencesAdipocyte physiologyCrown-like structuresAdipose tissueMacrophage populationsFunctional heterogeneityATM populationsTissue distributionHuman adipose tissueMacrophage subsetsPhysiological changesFat cellsAdoptive transferBody massTranscriptomeDonor tissue-specific exosome profiling enables noninvasive monitoring of acute rejection in mouse allogeneic heart transplantation
Habertheuer A, Korutla L, Rostami S, Reddy S, Lal P, Naji A, Vallabhajosyula P. Donor tissue-specific exosome profiling enables noninvasive monitoring of acute rejection in mouse allogeneic heart transplantation. Journal Of Thoracic And Cardiovascular Surgery 2018, 155: 2479-2489. PMID: 29499866, DOI: 10.1016/j.jtcvs.2017.12.125.Peer-Reviewed Original ResearchConceptsEarly acute rejectionDonor heartsAcute rejectionHeart transplantationStudy armsImmunologic rejectionMaintenance armHeterotopic heart transplantation modelAllogeneic heart transplantationTime pointsHeart transplantation modelHeterotopic heart transplantationAbsence of rejectionNovel biomarker platformDevelopment of biomarkersNoninvasive monitoringTransplant heartCardiac allograftsImmunocompetent recipientsAllograft monitoringImmunodeficient recipientsRecipient circulationTransplantation modelDay 1Total plasma
2017
Effect of the Diabetic State on Islet Engraftment and Function in a Large Animal Model of Islet–Kidney Transplantation
Vallabhajosyula P, Hirakata A, Weiss M, Griesemer A, Shimizu A, Hong H, Habertheuer A, Tchipashvili V, Yamada K, Sachs DH. Effect of the Diabetic State on Islet Engraftment and Function in a Large Animal Model of Islet–Kidney Transplantation. Cell Transplantation 2017, 26: 1755-1762. PMID: 29338381, PMCID: PMC5784526, DOI: 10.1177/0963689717732993.Peer-Reviewed Original ResearchConceptsDiabetic stateIslet transplantationLarge animal modelEngraftment periodIslet engraftmentControl donorsIslet equivalentsAnimal modelsLong-term islet functionSimilar glucose tolerance testsDonor animalsChronic diabetic stateEnd-organ injuryAutologous islet transplantationGlucose tolerance testMiniature swine modelNephrectomized recipientsTotal pancreatectomyDiabetic nephropathyRecipient weightGlycemic controlOrgan injuryTolerance inductionDonor weightIschemic factorsTissue-specific exosome biomarkers for noninvasively monitoring immunologic rejection of transplanted tissue
Vallabhajosyula P, Korutla L, Habertheuer A, Yu M, Rostami S, Yuan CX, Reddy S, Liu C, Korutla V, Koeberlein B, Trofe-Clark J, Rickels MR, Naji A. Tissue-specific exosome biomarkers for noninvasively monitoring immunologic rejection of transplanted tissue. Journal Of Clinical Investigation 2017, 127: 1375-1391. PMID: 28319051, PMCID: PMC5373894, DOI: 10.1172/jci87993.Peer-Reviewed Original ResearchConceptsRenal transplantationImmunologic rejectionTransplanted tissueClinical settingBiomarker platformAppearance of hyperglycemiaIslet transplant modelIslet β-cellsDonor exosomesTransplant isletsHLA antibodiesRecipient bloodTransplant modelRecipient circulationXenogeneic modelRecipient plasmaRenal epithelial cellsTransplant tissueHuman isletsΒ-cellsBiomarker potentialTransplantationNoninvasive windowEpithelial cellsExosomal microRNAs
2015
Monitoring of Allogeneic Islet Grafts in Nonhuman Primates Using MRI
Wang P, Schuetz C, Vallabhajosyula P, Medarova Z, Tena A, Wei L, Yamada K, Deng S, Markmann JF, Sachs DH, Moore A. Monitoring of Allogeneic Islet Grafts in Nonhuman Primates Using MRI. Transplantation 2015, 99: 1574-1581. PMID: 25806407, PMCID: PMC4551574, DOI: 10.1097/tp.0000000000000682.Peer-Reviewed Original ResearchMeSH KeywordsAcute DiseaseAnimalsBiomarkersBlood GlucoseContrast MediaDextransDisease Models, AnimalGraft RejectionImmunosuppressive AgentsIslets of LangerhansIslets of Langerhans TransplantationMagnetic Resonance ImagingMagnetite NanoparticlesMaleOrgan SizePapio hamadryasPredictive Value of TestsStreptozocinTime FactorsTransplantation, HomologousConceptsGraft volumeIslet graftsBlood glucosePancreatic isletsNonhuman primatesAcute graft lossAllogeneic immune rejectionAllogeneic islet graftsWithdrawal of immunosuppressionPancreatic islet graftsVivo MRIGraft lossAllogeneic isletsGraft monitoringTreatment optionsIslet lossIslet transplantationRenal capsuleAllogeneic modelAutologous modelImmune rejectionMRISecondary damageImmunosuppressionIslets
2013
Assessing the Effect of Immunosuppression on Engraftment of Pancreatic Islets
Vallabhajosyula P, Hirakata A, Shimizu A, Okumi M, Tchipashvili V, Hong H, Yamada K, Sachs DH. Assessing the Effect of Immunosuppression on Engraftment of Pancreatic Islets. Transplantation 2013, 96: 372-378. PMID: 23883972, PMCID: PMC3786704, DOI: 10.1097/tp.0b013e31829f7515.Peer-Reviewed Original ResearchConceptsTriple-drug immunosuppressionIslet functionAllogeneic islet cell transplantationExperimental animalsLong-term islet functionTriple-drug immunosuppressive regimenIntravenous glucose tolerance testEquivalent glycemic controlEffect of immunosuppressionGlucose tolerance testNormal glucose regulationIslet cell transplantationVivo large animal modelLong-term functionIslet equivalents/Large animal modelImmunosuppressive regimenIslet doseImmunologic factorsRecipient weightEngrafted isletsGlycemic controlMinor histocompatibilityImmunosuppressive drugsCell transplantation
2011
Signal Joint T-Cell Receptor Excision Circle Assay in Miniature Swine
Vallabhajosyula P, Tena A, Yamada K, Sachs DH. Signal Joint T-Cell Receptor Excision Circle Assay in Miniature Swine. Transplantation 2011, 92: 634-640. PMID: 21822172, PMCID: PMC3291197, DOI: 10.1097/tp.0b013e31822b9287.Peer-Reviewed Original ResearchComposite Islet-Kidneys From Single Baboon Donors Cure Diabetes Across Fully Allogenic Barriers
Yamada K, Hirakata A, Tchipashvili V, Shimizu A, Iwaki H, Griesemer A, Vallabhajosyula P, Moran S, Sachs DH. Composite Islet-Kidneys From Single Baboon Donors Cure Diabetes Across Fully Allogenic Barriers. American Journal Of Transplantation 2011, 11: 2603-2612. PMID: 21929644, PMCID: PMC3226882, DOI: 10.1111/j.1600-6143.2011.03733.x.Peer-Reviewed Original ResearchConceptsInsulin therapyFree isletsRenal capsuleEnd-stage diabetic nephropathyNonhuman primate modelPotential clinical applicabilityImmunosuppressive regimenAllogeneic barriersTotal pancreatectomyDiabetic nephropathyFBS levelsDiabetic hyperglycemiaPrimate modelPartial pancreatectomyDonor isletsMiniature swineNormal rangePotential cureGlucose regulationClinical applicabilityIsletsPancreatectomyTherapyIKsRecipientsQuantification of Baboon Thymopoiesis in Porcine Thymokidney Xenografts by the Signal-Joining T-Cell Receptor Excision Circle Assay
Tena A, Vallabhajosyula P, Hawley RJ, Griesemer A, Yamada K, Sachs DH. Quantification of Baboon Thymopoiesis in Porcine Thymokidney Xenografts by the Signal-Joining T-Cell Receptor Excision Circle Assay. Transplantation 2011, 91: 639-644. PMID: 21285918, PMCID: PMC3255479, DOI: 10.1097/tp.0b013e31820b6b52.Peer-Reviewed Original ResearchConceptsThymic tissueT cellsPeripheral bloodSjTREC levelsT-cell receptor excision circlesPeripheral blood mononuclear cellsPeripheral T cell poolT-cell receptor gene rearrangementsMemory T cellsT cell poolBlood mononuclear cellsNaïve T cellsReceptor gene rearrangementsQuantitative PCRDiscordant pigNative thymusThymic biopsiesGraft survivalKidney transplantPolymerase chain reaction analysisExcision circlesMononuclear cellsSurgical removalChain reaction analysisXenogeneic barriers
2009
In Vivo Imaging of Autologous Islet Grafts in the Liver and Under the Kidney Capsule in Non-Human Primates
Medarova Z, Vallabhajosyula P, Tena A, Evgenov N, Pantazopoulos P, Tchipashvili V, Weir G, Sachs D, Moore A. In Vivo Imaging of Autologous Islet Grafts in the Liver and Under the Kidney Capsule in Non-Human Primates. Transplantation 2009, 87: 1659-1666. PMID: 19502957, PMCID: PMC2746764, DOI: 10.1097/tp.0b013e3181a5cbc0.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisContrast MediaDextransFerrosoferric OxideInsulinInsulin SecretionIronIslets of Langerhans TransplantationKidneyLiver TransplantationMagnetic Resonance ImagingMagnetite NanoparticlesOxidesPancreatectomyPapioSubrenal Capsule AssayTransplantation, AutologousTransplantation, HeterologousConceptsMagnetic resonance imagingSuperparamagnetic iron oxide contrast agentIslet graftsIron oxide contrast agentNon-human primatesRenal subcapsular islet graftsNon-human primate modelIslet graft survivalVivo magnetic resonance imagingIntrahepatic graftsGraft survivalPosttransplantation periodKidney capsuleIslet transplantationPrimate modelRenal capsulePartial pancreatectomyLiver parenchymaContrast agentsDay 1Drug AdministrationIslet labelingClinical situationsHuman isletsResonance imaging
2007
Vascularized composite islet-kidney transplantation in a miniature swine model
Vallabhajosyula P, Griesemer A, Yamada K, Sachs DH. Vascularized composite islet-kidney transplantation in a miniature swine model. Cell Biochemistry And Biophysics 2007, 48: 201-207. PMID: 17709890, DOI: 10.1007/s12013-007-0027-4.Peer-Reviewed Original ResearchConceptsThymic tissueIslet equivalentsEnd-stage diabetic nephropathyType I diabetic patientsAllogeneic thymic tissueRecipient body weightLong-term toleranceLong-term survivalMiniature swine modelKidney allograftsMHC barriersDiabetic nephropathyAllogeneic isletsAllogeneic transplantationDiabetic hyperglycemiaDiabetic patientsThymic componentsTreatment regimensPortal veinRenal capsuleOrgan transplantsAutologous isletsMiniature swineTransplantationBody weight