2020
Oncometabolites suppress DNA repair by disrupting local chromatin signalling
Sulkowski PL, Oeck S, Dow J, Economos NG, Mirfakhraie L, Liu Y, Noronha K, Bao X, Li J, Shuch BM, King MC, Bindra RS, Glazer PM. Oncometabolites suppress DNA repair by disrupting local chromatin signalling. Nature 2020, 582: 586-591. PMID: 32494005, PMCID: PMC7319896, DOI: 10.1038/s41586-020-2363-0.Peer-Reviewed Original ResearchConceptsDNA repairDNA breaksFumarate hydrataseDownstream repair factorsHistone 3 lysine 9Homology-dependent repairPoly (ADP-ribose) polymeraseRecruitment of TIP60Deregulation of metabolismChromatin signalingSuccinate dehydrogenase genesGenome integrityLysine 9Repair factorsDehydrogenase geneEnd resectionIsocitrate dehydrogenase 1Aberrant hypermethylationMechanistic basisSomatic mutationsDehydrogenase 1GenesHuman malignanciesProper executionMutations
2016
Precise Genome Modification Using Triplex Forming Oligonucleotides and Peptide Nucleic Acids
Bahal R, Gupta A, Glazer P. Precise Genome Modification Using Triplex Forming Oligonucleotides and Peptide Nucleic Acids. Advances In Experimental Medicine And Biology 2016, 93-110. DOI: 10.1007/978-1-4939-3509-3_6.Peer-Reviewed Original ResearchHomologous recombinationSite-specific genome editingPrecise genome modificationGenomic DNA sequencesSpecific genome editingTriplex technologySingle base pair mutationTriplex forming oligonucleotideBase pair mutationGenetic disordersGenome modificationNucleic acidsDefective protein synthesisGenome editingDNA sequencesDifferent genesPair mutationsProtein synthesisMutation sitesGenesSpecific sitesGene replacement therapyExpressionOligonucleotideMutagenesis
2006
Peptide Nucleic Acids as Agents to Modify Target Gene Expression and Function
Wang G, Glazer P. Peptide Nucleic Acids as Agents to Modify Target Gene Expression and Function. Medical Intelligence Unit 2006, 223-235. DOI: 10.1007/0-387-32956-0_14.Peer-Reviewed Original ResearchTarget gene expressionGene expressionMolecular mechanismsHuman diseasesGene expression regulationExpression regulationTranscription initiationDNA repairImportant genesMolecular basisTranscriptionGenesNucleic acidsExpressionAbility of PNAMutagenesisAttractive strategyPowerful methodBetter understandingRegulationMechanismPeptide nucleic acidBroad range
2004
Targeted Genome Modification Via Triple Helix Formation
Rogers F, Glazer P. Targeted Genome Modification Via Triple Helix Formation. Cancer Drug Discovery And Development 2004, 27-43. DOI: 10.1007/978-1-59259-777-2_3.Peer-Reviewed Original ResearchDefective geneTargeted genome modificationInhibitors of proteinGene functionGenome modificationGene productsGene expressionTriple helix formationTremendous clinical valueSynthetic oligonucleotidesGenesInherited diseaseHelix formationTreatment of diseasesExpressionDifferent diseasesProteinTherapeutic agentsOligonucleotideTherapeutic useTremendous stridesInhibitorsFunction
2001
Triplex forming oligonucleotides: sequence-specific tools for gene targeting
Knauert M, Glazer P. Triplex forming oligonucleotides: sequence-specific tools for gene targeting. Human Molecular Genetics 2001, 10: 2243-2251. PMID: 11673407, DOI: 10.1093/hmg/10.20.2243.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsHuman gene therapyGene therapy agentsAbility of TFOsTriplex formingGenome modificationGene therapyMammalian cellsGenetic manipulationGene targetingGene expressionPotential applicationsGenetic targetingDuplex DNATherapy agentsMajor grooveLoose canonsHigh specificityGenesRecent studiesTargetingRelated moleculesTFOCellsDevicesDNADirected gene modification via triple helix formation.
Gorman L, Glazer P. Directed gene modification via triple helix formation. 2001, 1: 391-9. PMID: 11899085, DOI: 10.2174/1566524013363771.Peer-Reviewed Original ResearchConceptsGene modificationNon-functional gene productMammalian genesGene productsGenomic DNASingle nucleotideDefective geneTriple helix formationGenetic diseasesTriplex formingGenesHelix formationEfficient targetingNucleic acidsDNAInitial stepGene therapyCorrect sequenceNucleotidesMutationsMoleculesImportant advancesSequenceTargetingModification
2000
Specific Mutations Induced by Triplex-Forming Oligonucleotides in Mice
Vasquez K, Narayanan L, Glazer P. Specific Mutations Induced by Triplex-Forming Oligonucleotides in Mice. Science 2000, 290: 530-533. PMID: 11039937, DOI: 10.1126/science.290.5491.530.Peer-Reviewed Original ResearchConceptsSomatic cellsSpecific genomic sitesEmbryonic stem cell technologyDuplex DNA sequencesGene functionGreater mutation frequenciesGenomic sitesGenome modificationChromosomal copyDNA sequencesSequence-controlled oligomersReporter geneStem cell technologyControl genesGerm-line mutationsGenesSpecific mutationsSupF geneControl oligomersMutationsMutation frequencyTransgenic miceOligonucleotideCellsMutation detection
1999
Peptide nucleic acid (PNA) binding-mediated induction of human γ-globin gene expression
Wang G, Xu X, Pace B, Dean D, Glazer P, Chan P, Goodman S, Shokolenko I. Peptide nucleic acid (PNA) binding-mediated induction of human γ-globin gene expression. Nucleic Acids Research 1999, 27: 2806-2813. PMID: 10373600, PMCID: PMC148492, DOI: 10.1093/nar/27.13.2806.Peer-Reviewed Original ResearchConceptsGamma-globin gene expressionGamma-globin geneD-loop structureGene expressionHuman γ-globin gene expressionΓ-globin gene expressionGenetic diseasesK562 human erythroleukemia cellsGene expression strategyReporter gene constructsSequence-specific mannerBeta-globin geneHuman erythroleukemia cellsInduction of expressionAdult blood cellsEndogenous genesCommon genetic diseaseGene productsGene constructsExpression strategyErythroleukemia cellsHomopurine/homopyrimidine sequencesHuman diseasesGenesGlobin disordersTargeted Correction of an Episomal Gene in Mammalian Cells by a Short DNA Fragment Tethered to a Triplex-forming Oligonucleotide*
Chan P, Lin M, Faruqi A, Powell J, Seidman M, Glazer P. Targeted Correction of an Episomal Gene in Mammalian Cells by a Short DNA Fragment Tethered to a Triplex-forming Oligonucleotide*. Journal Of Biological Chemistry 1999, 274: 11541-11548. PMID: 10206960, DOI: 10.1074/jbc.274.17.11541.Peer-Reviewed Original ResearchConceptsMammalian cellsGene correctionSV40 shuttle vectorTriplex motifNucleotide excision repairSequence-specific mannerEpisomal geneSupF reporter geneXPA cDNASpecific target sitesGene conversionShort DNA fragmentsDNA repairGene targetingTarget genesDNA segmentsReporter geneExcision repairDNA fragmentsBifunctional oligonucleotidesShuttle vectorSequence conversionGenesSV40 vectorsFlexible linkerGenome Modification by Triplex-Forming Oligonucleotides
Vasquez K, Glazer P. Genome Modification by Triplex-Forming Oligonucleotides. Perspectives In Antisense Science 1999, 2: 167-179. DOI: 10.1007/978-1-4615-5177-5_13.Peer-Reviewed Original ResearchSite-specific genome modificationGenome modificationSpecific DNA sequencesExpression of genesLevel of DNAMammalian cellsHomologous recombinationDNA sequencesGene replacementGene expressionLiving cellsTriplex technologyTriplex formingSpecific sitesDNARecombinationExpressionInitial stepCellsMutagenesisGenesMutationsModificationSitesSequence
1997
Role of DNA mismatch repair in the cytotoxicity of ionizing radiation.
Fritzell J, Narayanan L, Baker S, Bronner C, Andrew S, Prolla T, Bradley A, Jirik F, Liskay R, Glazer P. Role of DNA mismatch repair in the cytotoxicity of ionizing radiation. Cancer Research 1997, 57: 5143-7. PMID: 9371516.Peer-Reviewed Original ResearchConceptsMammalian cellsCellular responsesCell linesTranscription-coupled repairMMR systemWild-type cellsDNA-damaging agentsWild-type cell linesMMR-deficient cellsDNA mismatch repairDNA mismatch repair systemMismatch repair systemActive genesFutile repairMMR factorsAlkylation damageMismatch repairReplication errorsDNA damageRepair systemRelated miceCancer cellsClonogenic survivalMMR genesGenesElevated levels of mutation in multiple tissues of mice deficient in the DNA mismatch repair gene Pms2
Narayanan L, Fritzell J, Baker S, Liskay R, Glazer P. Elevated levels of mutation in multiple tissues of mice deficient in the DNA mismatch repair gene Pms2. Proceedings Of The National Academy Of Sciences Of The United States Of America 1997, 94: 3122-3127. PMID: 9096356, PMCID: PMC20332, DOI: 10.1073/pnas.94.7.3122.Peer-Reviewed Original ResearchConceptsDNA mismatch repair gene PMS2Multiple tissuesMutation reporter geneMismatch repair gene PMS2Role of mutagenesisMammalian homologGenomic integrityReporter geneRepeat sequencesPMS2 locusMononucleotide repeat sequencesGenetic instabilityLimited tissue distributionDNA mismatch repair genesRepair genesHereditary colon cancerNormal developmentSlippage errorsGenesMutagenic treatmentEssential roleMismatch repair genesMutagenesisMutation frequencyHybrid transgenic micePotassium-Resistant Triple Helix Formation and Improved Intracellular Gene Targeting by Oligodeoxyribonucleotides Containing 7-Deazaxanthine
Faruqi A, Krawczyk S, Matteucci M, Glazer P. Potassium-Resistant Triple Helix Formation and Improved Intracellular Gene Targeting by Oligodeoxyribonucleotides Containing 7-Deazaxanthine. Nucleic Acids Research 1997, 25: 633-640. PMID: 9016606, PMCID: PMC146453, DOI: 10.1093/nar/25.3.633.Peer-Reviewed Original ResearchConceptsTriple helix-forming oligonucleotidesTriple helix formationGel mobility shift assaysHelix formationMutation reporter geneMobility shift assaysMammalian cellsAnti-gene strategyHigh mutation frequencyShift assaysGene targetingReporter geneGenesPhosphodiester backboneMutation frequencyPsoralen adductsVivo geneTriplex formationPhysiological concentrationsNucleotide chemistry
1995
Mutagenesis by 8-methoxypsoralen and 5-methylangelicin photoadducts in mouse fibroblasts: mutations at cross-linkable sites induced by offoadducts as well as cross-links.
Gunther E, Yeasky T, Gasparro F, Glazer P. Mutagenesis by 8-methoxypsoralen and 5-methylangelicin photoadducts in mouse fibroblasts: mutations at cross-linkable sites induced by offoadducts as well as cross-links. Cancer Research 1995, 55: 1283-8. PMID: 7882323.Peer-Reviewed Original ResearchConceptsLambda phage shuttle vectorMutation reporter geneMammalian cellsCross-linkable sitesFibroblast cell lineMouse fibroblast cell lineReporter geneMolecular eventsShuttle vectorSpectrum of mutationsMouse fibroblastsPremutagenic lesionsSupF geneCross-link formationMutationsC transversionCell linesMutagenesisGenesApt sitePsoralen treatmentCellsSitesDNA
1986
Detection and analysis of UV-induced mutations in mammalian cell DNA using a lambda phage shuttle vector.
Glazer P, Sarkar S, Summers W. Detection and analysis of UV-induced mutations in mammalian cell DNA using a lambda phage shuttle vector. Proceedings Of The National Academy Of Sciences Of The United States Of America 1986, 83: 1041-1044. PMID: 2937054, PMCID: PMC323006, DOI: 10.1073/pnas.83.4.1041.Peer-Reviewed Original ResearchConceptsMammalian cellsStable mouse L cell lineLambda phage shuttle vectorMouse cell DNACell DNAMammalian cell DNASupF geneMouse L cell lineDNA sequence analysisLambda phage vectorL cell lineMutant phageMouse cellsSequence analysisShuttle vectorUV-induced mutationsMultiple copiesUV mutagenesisPhage vectorEscherichia coliMutagenesisViable phagePhagesGenesT transition
1985
Direct and inducible mutagenesis in mammalian cells.
Summers W, Sarkar S, Glazer P. Direct and inducible mutagenesis in mammalian cells. Cancer Surveys 1985, 4: 517-28. PMID: 3916654.Peer-Reviewed Original ResearchConceptsMammalian cellsAnimal cellsAnimal virusesSimple eukaryotesInducible mutagenesisMutant geneSequence analysisShuttle vectorMutagenic pathwayMutagenesisDNA damageViral genomeWeigle mutagenesisMutagenic mechanismsDirect sequence analysisProkaryotesCellsBacteriaEukaryotesGenomeSimilar sitesRecent dataGenesExtrapolation of resultsPhages