Assembly of MHC class I molecules with biosynthesized endoplasmic reticulum-targeted peptides is inefficient in insect cells and can be enhanced by protease inhibitors.
Deng Y, Gibbs J, Bačík I, Porgador A, Copeman J, Lehner P, Ortmann B, Cresswell P, Bennink J, Yewdell J. Assembly of MHC class I molecules with biosynthesized endoplasmic reticulum-targeted peptides is inefficient in insect cells and can be enhanced by protease inhibitors. The Journal Of Immunology 1998, 161: 1677-85. PMID: 9712031, DOI: 10.4049/jimmunol.161.4.1677.Peer-Reviewed Original ResearchMeSH KeywordsAedesAnimalsAntibodies, MonoclonalAntiportersCell LineEndoplasmic ReticulumH-2 AntigensHeLa CellsHumansImmunoglobulinsLymphocyte ActivationMacromolecular SubstancesMembrane Transport ProteinsMiceOligopeptidesOvalbuminPeptide FragmentsProtease InhibitorsRecombinant ProteinsT-LymphocytesVaccinia virusConceptsInsect cellsEndoplasmic reticulumVertebrate cellsHuman cellsHuman tapasinVaccinia virus-mediated expressionCell surface expressionProtease inhibitorsInefficient assemblyKbMHC class IMouse betaInsectsEfficient assemblyImmediate precursorSurface expressionAntigenic peptidesHeavy chainClass IRecombinant vaccinia virusVirus-mediated expressionAssemblyExpressionCellsVaccinia virusCalnexin expression does not enhance the generation of MHC class I‐peptide complexes
Prasad S, Yewdell J, Porgador A, Sadasivan B, Cresswell P, Bennink J. Calnexin expression does not enhance the generation of MHC class I‐peptide complexes. European Journal Of Immunology 1998, 28: 907-913. PMID: 9541586, DOI: 10.1002/(sici)1521-4141(199803)28:03<907::aid-immu907>3.0.co;2-4.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, ViralATP Binding Cassette Transporter, Subfamily B, Member 2ATP-Binding Cassette TransportersBeta 2-MicroglobulinBiological TransportCalcium-Binding ProteinsCalnexinCell CompartmentationCells, CulturedH-2 AntigensHistocompatibility Antigens Class IHumansMacromolecular SubstancesMiceOvalbuminPeptidesProtein Binding