1998
Assembly of MHC class I molecules with biosynthesized endoplasmic reticulum-targeted peptides is inefficient in insect cells and can be enhanced by protease inhibitors.
Deng Y, Gibbs J, Bačík I, Porgador A, Copeman J, Lehner P, Ortmann B, Cresswell P, Bennink J, Yewdell J. Assembly of MHC class I molecules with biosynthesized endoplasmic reticulum-targeted peptides is inefficient in insect cells and can be enhanced by protease inhibitors. The Journal Of Immunology 1998, 161: 1677-85. PMID: 9712031, DOI: 10.4049/jimmunol.161.4.1677.Peer-Reviewed Original ResearchMeSH KeywordsAedesAnimalsAntibodies, MonoclonalAntiportersCell LineEndoplasmic ReticulumH-2 AntigensHeLa CellsHumansImmunoglobulinsLymphocyte ActivationMacromolecular SubstancesMembrane Transport ProteinsMiceOligopeptidesOvalbuminPeptide FragmentsProtease InhibitorsRecombinant ProteinsT-LymphocytesVaccinia virusConceptsInsect cellsEndoplasmic reticulumVertebrate cellsHuman cellsHuman tapasinVaccinia virus-mediated expressionCell surface expressionProtease inhibitorsInefficient assemblyKbMHC class IMouse betaInsectsEfficient assemblyImmediate precursorSurface expressionAntigenic peptidesHeavy chainClass IRecombinant vaccinia virusVirus-mediated expressionAssemblyExpressionCellsVaccinia virus
1992
HLA-DR molecules from an antigen-processing mutant cell line are associated with invariant chain peptides
Riberdy J, Newcomb J, Surman M, Barbosat J, Cresswell P. HLA-DR molecules from an antigen-processing mutant cell line are associated with invariant chain peptides. Nature 1992, 360: 474-477. PMID: 1448172, DOI: 10.1038/360474a0.Peer-Reviewed Original ResearchConceptsClass II moleculesInvariant chainMajor histocompatibility complex (MHC) class II moleculesCell linesHLA-DR moleculesClass II binding siteInvariant chain peptideHLA-DR3 moleculesMore MHCClass IIAntigen processingT2 transfectantsAntigenic peptidesHuman cell linesChain peptideVivo conditionsPeptidesLarge proportionProteolytic cleavageMutant cell linesCertain human cell linesEarly stagesTransfectants
1987
An epitope common to HLA class I and class II antigens, Ig light chains, and β2-microglobulin
Lutz P, Cresswell P. An epitope common to HLA class I and class II antigens, Ig light chains, and β2-microglobulin. Immunogenetics 1987, 25: 228-233. PMID: 2437022, DOI: 10.1007/bf00404692.Peer-Reviewed Original ResearchMeSH KeywordsAntibodies, MonoclonalBeta 2-MicroglobulinEpitopesHLA AntigensHLA-DR AntigensHumansImmunoglobulin Heavy ChainsImmunoglobulin Light ChainsConceptsB-lymphoblastoid cell linesClass I heavy chainsWestern blottingClass I major histocompatibility complex antigensClass II MHC antigensMajor histocompatibility complex antigensClass IHLA class I heavy chainsClass II antigensHistocompatibility complex antigensHLA class IBeta-2-microglobulinLight chainHeavy chainNormal human serumWestern blot analysisMHC antigensII antigensComplex antigensImmunoglobulin light chainsM1 mAbΒ2-microglobulinMonoclonal antibodiesAntigenHuman immunoglobulin
1986
Expression of differentiation antigens by hybrids of human lymphoblastoid cells
Howell D, Salter R, Koppelman B, Cresswell P. Expression of differentiation antigens by hybrids of human lymphoblastoid cells. Human Immunology 1986, 17: 443-455. PMID: 3098709, DOI: 10.1016/0198-8859(86)90303-4.Peer-Reviewed Original ResearchConceptsParent cell lineCell linesClass IClass II antigensB lymphocyte antigensClass II histocompatibility antigensLymphocyte differentiation markersT-lymphoblastoid cell lineII antigensHistocompatibility antigensLymphocyte antigenDifferentiation antigensLymphoblastoid cell linesParental antigensHNK-1AntigenMonoclonal antibodiesHuman lymphoblastoid cellsT-LCLDifferentiation markersLymphoblastoid cellsCurrent study
1984
Analysis of monoclonal antibodies reactive with human class II beta chains by two-dimensional electrophoresis and western blotting
Radka S, Machamer C, Cresswell P. Analysis of monoclonal antibodies reactive with human class II beta chains by two-dimensional electrophoresis and western blotting. Human Immunology 1984, 10: 177-188. PMID: 6611328, DOI: 10.1016/0198-8859(84)90038-7.Peer-Reviewed Original ResearchConceptsMonomorphic monoclonal antibodiesClass II moleculesMonoclonal antibodiesBeta chainClass II beta chainPolymorphic monoclonal antibodiesHLA-DR5HLA-DRMonomorphic determinantsBeta subunitDeterminant absentPolymorphic antibodiesWestern blottingAntibodiesAntigen subunitsTwo-dimensional gel electrophoresisTwo-dimensional electrophoresisDR3Expression of class I histocompatibility antigens on human T-B lymphoblast hybrids
Howell D, Kostyu D, Ting J, Cresswell P. Expression of class I histocompatibility antigens on human T-B lymphoblast hybrids. Somatic Cell And Molecular Genetics 1984, 10: 217-224. PMID: 6609440, DOI: 10.1007/bf01535244.Peer-Reviewed Original ResearchMeSH KeywordsAntibodies, MonoclonalB-LymphocytesCell LineFlow CytometryHLA AntigensHLA-A AntigensHLA-B AntigensHumansHybrid CellsT-LymphocytesConceptsClass I histocompatibilityB-LCLLymphoblastoid cell linesB antigensPaucity of HLAHLA class I heavy chainsHLA-B antigensHLA-A antigensB-lymphoblastoid cell linesB antigen expressionClass I heavy chainsAntigen expressionB expressionHLAIndirect immunofluorescenceAntigenLow levelsClass IT-LCLHistocompatibilityCell linesHeavy chain mRNATotal levelsCEMRHeavy chainHLA-DR7-Specific monoclonal antibodies and a chimpanzee anti-DR7 serum detect different epitopes on the same molecule
Radka S, Bernard Amos D, Quackenbush L, Cresswell P. HLA-DR7-Specific monoclonal antibodies and a chimpanzee anti-DR7 serum detect different epitopes on the same molecule. Immunogenetics 1984, 19: 63-76. PMID: 6198273, DOI: 10.1007/bf00364476.Peer-Reviewed Original ResearchConceptsClass II moleculesChimpanzee antiserumSame class II moleculesDR7 specificitiesSerologic specificityMonoclonal antibodiesBinding-inhibition studiesCell linesSame serologic specificityAllogeneic epitopesNoncytotoxic antibodiesHLA-DR7IgM antibodiesSpecific monoclonal antibodiesDR moleculesDifferent epitopesAntibodiesEpitopesBeta chainCytotoxic activityHomozygous cell linesAntiserumMembrane preparationsCytotoxicitySpecificity
1983
Expression of T-lymphoblast-encoded HLA-DR, MT, and SB antigens on human T-B lymphoblast hybrids
Howell D, Hartzman R, Cresswell P. Expression of T-lymphoblast-encoded HLA-DR, MT, and SB antigens on human T-B lymphoblast hybrids. Human Immunology 1983, 8: 167-175. PMID: 6417070, DOI: 10.1016/0198-8859(83)90011-3.Peer-Reviewed Original ResearchExpression of T-lymphoblast-encoded HLA-DR antigens on human T-B lymphoblast hybrids
Howell D, Cresswell P. Expression of T-lymphoblast-encoded HLA-DR antigens on human T-B lymphoblast hybrids. Immunogenetics 1983, 17: 411-425. PMID: 6601057, DOI: 10.1007/bf00372459.Peer-Reviewed Original Research
1982
Elimination of mycoplasma from human B-lymphoblastoid cell lines
Howell D, Machamer C, Cresswell P. Elimination of mycoplasma from human B-lymphoblastoid cell lines. Human Immunology 1982, 5: 233-238. PMID: 6983518, DOI: 10.1016/0198-8859(82)90136-7.Peer-Reviewed Original ResearchMonoclonal Antibody to HLA-A3
BERGER A, DAVIS J, CRESSWELL P. Monoclonal Antibody to HLA-A3. Monoclonal Antibodies In Immunodiagnosis And Immunotherapy 1982, 1: 87-90. PMID: 6208124, DOI: 10.1089/hyb.1.1982.1.87.Peer-Reviewed Original Research
1981
A human leukocyte antigen identified by a monoclonal antibody
Berger A, Davis J, Cresswell P. A human leukocyte antigen identified by a monoclonal antibody. Human Immunology 1981, 3: 231-245. PMID: 6975769, DOI: 10.1016/0198-8859(81)90020-3.Peer-Reviewed Original ResearchConceptsLeukocyte antigenT cell linesB cell linesB lymphocytesCell linesMonoclonal antibodiesHuman leukocyte antigenPeripheral blood cellsBone marrow cellsAllogeneic cellsT cellsB cellsErythroid seriesMore antigensAntigenHuman leukocytesMarrow cellsBlood cellsNegative populationAntibodiesLymphocytesApproximate molecular weightCellsElectrophoretic mobilityMonocytes
1980
A public HLA antigen associated with HLA-A9, Aw32, and Bw4
Kostyu D, Cresswell P, Amos D. A public HLA antigen associated with HLA-A9, Aw32, and Bw4. Immunogenetics 1980, 10: 433-442. PMID: 22457917, DOI: 10.1007/bf01572579.Peer-Reviewed Original ResearchMeSH KeywordsAntibodies, MonoclonalCytotoxicity Tests, ImmunologicEpitopesGlycoproteinsHLA-A AntigensHLA-B AntigensHumansLinkage DisequilibriumLymphocytes