2001
A Role for Calnexin in the Assembly of the MHC Class I Loading Complex in the Endoplasmic Reticulum
Diedrich G, Bangia N, Pan M, Cresswell P. A Role for Calnexin in the Assembly of the MHC Class I Loading Complex in the Endoplasmic Reticulum. The Journal Of Immunology 2001, 166: 1703-1709. PMID: 11160214, DOI: 10.4049/jimmunol.166.3.1703.Peer-Reviewed Original ResearchAntiportersATP Binding Cassette Transporter, Subfamily B, Member 2ATP-Binding Cassette TransportersCalcium-Binding ProteinsCalnexinCalreticulinCell Line, TransformedDimerizationEndoplasmic ReticulumHeat-Shock ProteinsHeLa CellsHistocompatibility Antigens Class IHLA AntigensHumansImmunoglobulinsIsomerasesKineticsMajor Histocompatibility ComplexMembrane Transport ProteinsProtein BindingProtein Disulfide-IsomerasesRibonucleoproteinsTumor Cells, Cultured
2000
Enzymatic reduction of disulfide bonds in lysosomes: Characterization of a Gamma-interferon-inducible lysosomal thiol reductase (GILT)
Arunachalam B, Phan U, Geuze H, Cresswell P. Enzymatic reduction of disulfide bonds in lysosomes: Characterization of a Gamma-interferon-inducible lysosomal thiol reductase (GILT). Proceedings Of The National Academy Of Sciences Of The United States Of America 2000, 97: 745-750. PMID: 10639150, PMCID: PMC15401, DOI: 10.1073/pnas.97.2.745.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceBinding SitesCOS CellsDisulfidesDNA, ComplementaryEndosomesEnzyme InductionHumansHydrogen-Ion ConcentrationInterferon-gammaLysosomesMannosephosphatesMicroscopy, ImmunoelectronMolecular Sequence DataMutagenesisOxidation-ReductionProtein Disulfide Reductase (Glutathione)Protein Processing, Post-TranslationalSequence Analysis, DNATumor Cells, CulturedConceptsGamma interferon inducible lysosomal thiol reductaseLysosomal thiol reductaseThiol reductaseDisulfide bondsC-terminal prosequenceEndocytic pathwayThioredoxin familyCysteine residuesDisulfide bond reductionEfficient proteolysisCell typesAmino acidsLysosomal systemEnzymeLysosomesSoluble glycoproteinReductaseActive siteBond reductionAntigen processingImportant roleEnzymatic reductionMutagenesisThioredoxinProsequence
1999
Cytomegalovirus US2 destroys two components of the MHC class II pathway, preventing recognition by CD4+ T cells
Tomazin R, Boname J, Hegde N, Lewinsohn D, Altschuler Y, Jones T, Cresswell P, Nelson J, Riddell S, Johnson D. Cytomegalovirus US2 destroys two components of the MHC class II pathway, preventing recognition by CD4+ T cells. Nature Medicine 1999, 5: 1039-1043. PMID: 10470081, DOI: 10.1038/12478.Peer-Reviewed Original ResearchConceptsHuman cytomegalovirusT cellsT lymphocytesClass II proteinsMHC class II antigen presentation pathwayMHC class II pathwayClass II antigen presentation pathwayHCMV-infected macrophagesAntigen presentation pathwayClass II pathwayLifelong latent infectionLife-threatening diseaseMonocytes/macrophagesMHC class IImportant host cellsMHC class II proteinsImmune evasion proteinsVirus reactivationHLA-DRLatent reservoirPresent antigensGlial cellsUbiquitous herpesvirusLatent infectionPresentation pathway
1997
Negative Regulation by HLA-DO of MHC Class II-Restricted Antigen Processing
Denzin L, Sant'Angelo D, Hammond C, Surman M, Cresswell P. Negative Regulation by HLA-DO of MHC Class II-Restricted Antigen Processing. Science 1997, 278: 106-109. PMID: 9311912, DOI: 10.1126/science.278.5335.106.Peer-Reviewed Original ResearchConceptsHLA-DOII-like moleculeAntigen processingClass IIHLA-DMMajor histocompatibility complex classMHC class IIHistocompatibility complex classClass II moleculesII-CLIP complexesHLA-DM functionT cell linesB cellsChain-derived peptideAntigenic peptidesImportant modulatorCell linesDM functionInvariant chain-derived peptidesComplex classNegative regulationPeptidesA Critical Role for Tapasin in the Assembly and Function of Multimeric MHC Class I-TAP Complexes
Ortmann B, Copeman J, Lehner P, Sadasivan B, Herberg J, Grandea A, Riddell S, Tampé R, Spies T, Trowsdale J, Cresswell P. A Critical Role for Tapasin in the Assembly and Function of Multimeric MHC Class I-TAP Complexes. Science 1997, 277: 1306-1309. PMID: 9271576, DOI: 10.1126/science.277.5330.1306.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAntigen PresentationAntiportersATP Binding Cassette Transporter, Subfamily B, Member 2ATP Binding Cassette Transporter, Subfamily B, Member 3ATP-Binding Cassette TransportersCalcium-Binding ProteinsCalreticulinCell LineCell Line, TransformedChromosome MappingChromosomes, Human, Pair 6Cloning, MolecularDimerizationEndoplasmic ReticulumGenetic LinkageHistocompatibility Antigens Class IHLA AntigensHumansImmunoglobulin GImmunoglobulinsMajor Histocompatibility ComplexMembrane Transport ProteinsMolecular Sequence DataRibonucleoproteinsSequence Homology, Amino AcidT-Lymphocytes, CytotoxicTumor Cells, CulturedMisfolded major histocompatibility complex class I heavy chains are translocated into the cytoplasm and degraded by the proteasome
Hughes E, Hammond C, Cresswell P. Misfolded major histocompatibility complex class I heavy chains are translocated into the cytoplasm and degraded by the proteasome. Proceedings Of The National Academy Of Sciences Of The United States Of America 1997, 94: 1896-1901. PMID: 9050876, PMCID: PMC20014, DOI: 10.1073/pnas.94.5.1896.Peer-Reviewed Original ResearchMeSH KeywordsAcetylcysteineBeta 2-MicroglobulinBlotting, WesternCysteine EndopeptidasesCysteine Proteinase InhibitorsCytoplasmEndoplasmic ReticulumEnzyme InhibitorsGlycosylationHistocompatibility Antigens Class IHumansKineticsLeupeptinsMultienzyme ComplexesProteasome Endopeptidase ComplexProtein FoldingSolubilityTransfectionTumor Cells, CulturedConceptsClass I heavy chainsMHC class I heavy chainMajor histocompatibility complex class I heavy chainsBeta2-microglobulinHeavy chainMHC class ICell linesCell line DaudiTAP-deficient cell linesSpecific irreversible inhibitorClass IHerpes simplex virus proteinDaudiVirus proteinsEndoplasmic reticulumIrreversible inhibitorSimilar accumulationLactacystinPeriod of hours
1996
HLA-DM Interactions with Intermediates in HLA-DR Maturation and a Role for HLA-DM in Stabilizing Empty HLA-DR Molecules
Denzin L, Hammond C, Cresswell P. HLA-DM Interactions with Intermediates in HLA-DR Maturation and a Role for HLA-DM in Stabilizing Empty HLA-DR Molecules. Journal Of Experimental Medicine 1996, 184: 2153-2166. PMID: 8976171, PMCID: PMC2196380, DOI: 10.1084/jem.184.6.2153.Peer-Reviewed Original ResearchConceptsClass II moleculesHLA-DR moleculesHLA-DMDR moleculesMajor histocompatibility complex classAntigenic peptidesEmpty class II moleculesCLIP dissociationHistocompatibility complex classMHC class II compartmentsHLA-DM moleculesClass II compartmentsII-CLIP complexesClass II dimersAlpha beta dimersAntigen processingBeta dimersDR complexesCell linesInvariant chain fragmentComplex classAssociationLow levels