Purification of papain-solubilized histocompatibility antigens from a cultured human lymphoblastoid line, RPMI 4265.
Turner M, Cresswell P, Parham P, Strominger J, Mann D, Sanderson A. Purification of papain-solubilized histocompatibility antigens from a cultured human lymphoblastoid line, RPMI 4265. Journal Of Biological Chemistry 1975, 250: 4512-4519. PMID: 1141219, DOI: 10.1016/s0021-9258(19)41332-x.Peer-Reviewed Original ResearchConceptsSodium dodecyl sulfate gel electrophoresisSephadex GDodecyl sulfate gel electrophoresisSulfate gel electrophoresisDiethylaminoethylcellulose columnSingle bandCharge heterogeneityM ureaPreparationDEAE-cellulose columnChromatographyM KClSeparationGel electrophoresisMultiple bandsColumnSialic acid