Multiple Defects in Erythroid Gene Expression in Erythroid Krupple-Like Factor (EKLF) Target Genes in EKLF-Deficient Mice.
Gallagher P, Pilon A, Arcasoy M, Bodine D. Multiple Defects in Erythroid Gene Expression in Erythroid Krupple-Like Factor (EKLF) Target Genes in EKLF-Deficient Mice. Blood 2004, 104: 1602. DOI: 10.1182/blood.v104.11.1602.1602.Peer-Reviewed Original ResearchDNaseI hypersensitive sitesSubtractive hybridizationHypersensitive sitesΒ-globin geneHistone H3Transcription factorsTarget genesFetal liver cellsΒ-spectrinGene expressionΒ-globinErythroid gene expressionMouse Genome 430 2.0 ArrayAffymetrix GeneChip Mouse Genome 430 2.0 ArrayFactor target genesAHSP gene expressionChromatin immunoprecipitation analysisBand 3Mature erythroid progenitorsAHSP promoterRBC membrane proteinsErythroid genesChromatin upstreamChromatin modulatorsRegulated genesChromatin Remodeling of the Mouse AHSP Gene Requires EKLF.
Pilon A, Wong C, Garrett-Beal L, Weiss M, Gallagher P, Bodine D. Chromatin Remodeling of the Mouse AHSP Gene Requires EKLF. Blood 2004, 104: 375. DOI: 10.1182/blood.v104.11.375.375.Peer-Reviewed Original ResearchAlpha-Hemoglobin Stabilizing ProteinDNase I hypersensitive sitesAHSP geneAHSP gene expressionHypersensitive sitesWild typeΓ-globin mRNAFetal liver cellsHistone H3Human γ-globin genesGene expressionStrong DNase I hypersensitive siteIntron 1Wild-type chromatinChromatin Remodeling ComplexErythroid-specific proteinΓ-globin genePutative promoter sequencesTranscription factor EKLFExon 1Non-coding exonsΑ-globin mRNAPosition-independent expressionMRNA initiation siteRNase protection analysis