Fgf8b-containing spliceforms, but not Fgf8a, are essential for Fgf8 function during development of the midbrain and cerebellum
Guo Q, Li K, Sunmonu N, Li J. Fgf8b-containing spliceforms, but not Fgf8a, are essential for Fgf8 function during development of the midbrain and cerebellum. Developmental Biology 2009, 338: 183-192. PMID: 19968985, PMCID: PMC2815264, DOI: 10.1016/j.ydbio.2009.11.034.Peer-Reviewed Original ResearchConceptsGain-of-function studiesMHB regionMutant miceLoss-of-function analysesSplice-site mutationDouble mutant miceLoss of Fgf8In vivo requirementFunction of FGF8Alternative splicingProtein isoformsRegulatory genesActivation of Fgf8Fgf8 geneN-terminiSpliceformsGrowth retardationPostnatal lethalityGenetic backgroundMiceMidbrainFGF17Fgf8aCerebellumPosterior midbrainMisexpression of Gbx2 throughout the mesencephalon by a conditional gain‐of‐function transgene leads to deletion of the midbrain and cerebellum in mice
Sunmonu N, Chen L, Li J. Misexpression of Gbx2 throughout the mesencephalon by a conditional gain‐of‐function transgene leads to deletion of the midbrain and cerebellum in mice. Genesis 2009, 47: 667-673. PMID: 19603509, PMCID: PMC2783588, DOI: 10.1002/dvg.20546.Peer-Reviewed Original ResearchConceptsTransgenic mouse linesMouse homeobox genesMidbrain-hindbrain junctionCre-mediated recombinationPersistent expressionAbsence of CreEmbryonic dayMouse linesMouse embryosAdult brainExpression of Gbx2MiceAnterior shiftHomeobox genesNeural tubeMidbrainFunctional studiesPosterior partCerebellumRhombomere 1Ectopic expressionMesencephalonOtx2Red fluorescent proteinBeta-galactosidase