2010
A Conserved Protein Interaction Interface on the Type 5 G Protein β Subunit Controls Proteolytic Stability and Activity of R7 Family Regulator of G Protein Signaling Proteins*
Porter MY, Xie K, Pozharski E, Koelle MR, Martemyanov KA. A Conserved Protein Interaction Interface on the Type 5 G Protein β Subunit Controls Proteolytic Stability and Activity of R7 Family Regulator of G Protein Signaling Proteins*. Journal Of Biological Chemistry 2010, 285: 41100-41112. PMID: 20959458, PMCID: PMC3003408, DOI: 10.1074/jbc.m110.163600.Peer-Reviewed Original ResearchConceptsR7 RGS proteinsG protein signaling (RGS) proteinsRGS proteinsDEP domainSignaling proteinsProtein interaction interfacesGenetic screenCaenorhabditis elegansRGS complexesObligate complexesProtein complexesFamily regulatorGβ5 proteinEquivalent mutationN-terminusConformational rearrangementsGβ5ProteinInteraction interfaceProteolysisMutationsRegulatorProteolytic stabilityComplexesDynamic opening
2009
RSBP-1 Is a Membrane-targeting Subunit Required by the Gαq-specific But Not the Gαo-specific R7 Regulator of G protein Signaling in Caenorhabditis elegans
Porter MY, Koelle MR. RSBP-1 Is a Membrane-targeting Subunit Required by the Gαq-specific But Not the Gαo-specific R7 Regulator of G protein Signaling in Caenorhabditis elegans. Molecular Biology Of The Cell 2009, 21: 232-243. PMID: 19923320, PMCID: PMC2808233, DOI: 10.1091/mbc.e09-07-0642.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCaenorhabditis elegansCaenorhabditis elegans ProteinsCell MembraneGTP-Binding Protein alpha Subunits, Gi-GoGTP-Binding Protein alpha Subunits, Gq-G11GTP-Binding Protein RegulatorsImmunoprecipitationLocomotionMembrane ProteinsMolecular Sequence DataMusclesMutationNervous SystemOvulationProtein TransportRGS ProteinsSequence AlignmentSequence Homology, Amino AcidSignal TransductionSubcellular FractionsTransgenesConceptsR7 RGS proteinsRGS proteinsCaenorhabditis elegansEGL-10EAT-16G protein signaling (RGS) proteinsG proteinsMembrane-targeting sequenceGalpha GTPase activityC. elegans neuronsPhenocopies lossR7 regulatorMembrane associationRGS activityMembrane localizationProtein familyR7 familySignaling proteinsGTPase activityPlasma membraneGenetic studiesCultured cellsProteinR7BPElegansChapter 2 Insights into RGS Protein Function from Studies in Caenorhabditis elegans
Porter MY, Koelle MR. Chapter 2 Insights into RGS Protein Function from Studies in Caenorhabditis elegans. Progress In Nucleic Acid Research And Molecular Biology 2009, 86: 15-47. PMID: 20374712, DOI: 10.1016/s1877-1173(09)86002-x.Peer-Reviewed Original ResearchConceptsRGS proteinsGα proteinsMultiple RGS proteinsRGS protein functionG protein αRGS domainCaenorhabditis elegansProtein subfamiliesC. elegansGα genesProtein functionChapter 2 InsightsVivo functionNematode wormsPhysiological functionsProtein αProteinElegansSubfamiliesSuch specificityDifferent cellsRGSOrthologsCaenorhabditisMammals
2007
C. elegans G Protein Regulator RGS-3 Controls Sensitivity to Sensory Stimuli
Ferkey DM, Hyde R, Haspel G, Dionne HM, Hess HA, Suzuki H, Schafer WR, Koelle MR, Hart AC. C. elegans G Protein Regulator RGS-3 Controls Sensitivity to Sensory Stimuli. Neuron 2007, 53: 39-52. PMID: 17196529, PMCID: PMC1855255, DOI: 10.1016/j.neuron.2006.11.015.Peer-Reviewed Original ResearchConceptsSignal transductionG protein-coupled signal transductionRGS-3G protein signaling (RGS) proteinsHeterotrimeric G proteinsSpecific RGS proteinsRGS proteinsSensory neuronsSignaling proteinsMutant animalsNegative regulatorCalcium-binding proteinsG proteinsCalcium signalingBehavioral defectsTransductionSpecific odorantsProteinDefective responseRegulatorSensory behaviorsSynaptic transmissionIntense sensory stimuliExternal stimuliSignaling
2004
RGS-7 Completes a Receptor-Independent Heterotrimeric G Protein Cycle to Asymmetrically Regulate Mitotic Spindle Positioning in C. elegans
Hess HA, Röper JC, Grill SW, Koelle MR. RGS-7 Completes a Receptor-Independent Heterotrimeric G Protein Cycle to Asymmetrically Regulate Mitotic Spindle Positioning in C. elegans. Cell 2004, 119: 209-218. PMID: 15479638, DOI: 10.1016/j.cell.2004.09.025.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCaenorhabditis elegansCaenorhabditis elegans ProteinsCell DivisionCentrosomeEmbryo, NonmammalianGTP-Binding Protein alpha SubunitsGuanine Nucleotide Exchange FactorsGuanosine TriphosphateHeterotrimeric GTP-Binding ProteinsMutationNuclear ProteinsProtein BindingRecombinant Fusion ProteinsRGS ProteinsRNA InterferenceSpindle ApparatusConceptsG protein functionRIC-8G proteinsProtein functionC. elegans embryosAsymmetric cell divisionG protein effectorsHeterotrimeric G proteinsMitotic spindle positioningG protein signalingG-protein cycleSeven-transmembrane receptorsGPR-1/2RGS domainElegans embryosGTPase activatorProtein effectorsProtein cycleMicrotubule forcesSpindle positioningProtein signalingCell cortexCell divisionGTP hydrolysisMitotic spindleMechanism of extrasynaptic dopamine signaling in Caenorhabditis elegans
Chase DL, Pepper JS, Koelle MR. Mechanism of extrasynaptic dopamine signaling in Caenorhabditis elegans. Nature Neuroscience 2004, 7: 1096-1103. PMID: 15378064, DOI: 10.1038/nn1316.Peer-Reviewed Original ResearchMeSH KeywordsAcetylcholineAnimalsCaenorhabditis elegansCaenorhabditis elegans ProteinsDNA, ComplementaryDopamineGene TargetingGTP-Binding Protein alpha Subunits, Gi-GoGTP-Binding Protein alpha Subunits, Gq-G11GTP-Binding ProteinsMolecular Sequence DataMotor ActivityMotor NeuronsMutationNervous SystemPhylogenyReceptors, DopamineReceptors, Dopamine D1Receptors, Dopamine D2RGS ProteinsSequence Homology, Amino AcidSequence Homology, Nucleic AcidSignal TransductionConceptsCaenorhabditis elegansDOP-3DOP-1D2-like receptorsSignaling ComplexC. elegans locomotionLocomotion defectsExtrasynaptic dopamineAntagonistic effectGαoGαqElegansD2-like dopamine receptorsD1-like receptorsSame motor neuronsPathwayReceptorsDopaminergic neuronsDopamine receptorsMotor neuronsMutantsGenesDopamineSubunitsRegulatorGenetic Analysis of RGS Protein Function in Caenorhabditis elegans
Chase DL, Koelle MR. Genetic Analysis of RGS Protein Function in Caenorhabditis elegans. Methods In Enzymology 2004, 389: 305-320. PMID: 15313573, DOI: 10.1016/s0076-6879(04)89018-9.Peer-Reviewed Original ResearchConceptsRGS proteinsC. elegansG proteinsRGS protein functionStructure/function studiesG protein geneCaenorhabditis elegansGalpha mutantsClose homologProtein functionGalpha proteinsElegansGenetic analysisDetailed protocolTransgenic expressionProteinMost mammaliansMutantsFunction studiesOrthologsCaenorhabditisHomologMammalianGenesOrganisms
2002
An N-terminal Region of Caenorhabditis elegans RGS Proteins EGL-10 and EAT-16 Directs Inhibition of Gαo VersusGαq Signaling*
Patikoglou GA, Koelle MR. An N-terminal Region of Caenorhabditis elegans RGS Proteins EGL-10 and EAT-16 Directs Inhibition of Gαo VersusGαq Signaling*. Journal Of Biological Chemistry 2002, 277: 47004-47013. PMID: 12354761, DOI: 10.1074/jbc.m208186200.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAnimals, Genetically ModifiedBlotting, WesternCaenorhabditis elegansCaenorhabditis elegans ProteinsCell MembraneChromosomesEpitopesGTP-Binding Protein RegulatorsHelminth ProteinsHeterotrimeric GTP-Binding ProteinsImmunoblottingModels, BiologicalMolecular Sequence DataMutationPlasmidsPromoter Regions, GeneticProtein BindingProtein Structure, TertiaryProteinsRGS ProteinsSequence Homology, Amino AcidSignal TransductionTime FactorsTransgenesConceptsN-terminal regionEGL-10EGL-30GOA-1EAT-16G protein signaling (RGS) proteinsN-terminalGPB-2RGS domainRGS proteinsC. elegansGbeta subunitsMembrane localizationSignaling proteinsN-terminal fragmentC-terminal fragmentGTPase activityTarget specificityBiochemical analysisProteinTarget selectivityFragment complexChimerasFragmentsDirect inhibition
2001
Two RGS proteins that inhibit Gαo and Gαq signaling in C. elegans neurons require a Gβ5-like subunit for function
Chase D, Patikoglou G, Koelle M. Two RGS proteins that inhibit Gαo and Gαq signaling in C. elegans neurons require a Gβ5-like subunit for function. Current Biology 2001, 11: 222-231. PMID: 11250150, DOI: 10.1016/s0960-9822(01)00071-9.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedBehavior, AnimalCaenorhabditis elegansCaenorhabditis elegans ProteinsGene ExpressionGTP-Binding Protein alpha Subunits, Gi-GoGTP-Binding Protein alpha Subunits, Gq-G11GTP-Binding Protein beta SubunitsGTP-Binding Protein RegulatorsGTP-Binding ProteinsHelminth ProteinsHeterotrimeric GTP-Binding ProteinsMiceNeuronsOvipositionRGS ProteinsSignal TransductionTransgenesConceptsRGS proteinsEGL-10EAT-16C. elegansG protein signaling (RGS) proteinsG protein heterotrimersC. elegans neuronsG protein signalingGPB-2Gbeta proteinsGTPase activatorNull mutantsRGS activityDouble mutantSignaling proteinsProtein signalingG proteinsPhysiological roleMutantsProteinKnockout animalsElegansObvious defectsRGSSubunits
2000
Multiple RGS proteins alter neural G protein signaling to allow C. elegans to rapidly change behavior when fed
Dong M, Chase D, Patikoglou G, Koelle M. Multiple RGS proteins alter neural G protein signaling to allow C. elegans to rapidly change behavior when fed. Genes & Development 2000, 14: 2003-2014. PMID: 10950865, PMCID: PMC316861, DOI: 10.1101/gad.14.16.2003.Peer-Reviewed Original ResearchConceptsRGS proteinsEGL-10Egg-laying behaviorG proteinsRGS-2RGS-1Mammalian RGS proteinsMultiple RGS proteinsHeterotrimeric G proteinsG protein GTPase activityG protein signalingProtein GTPase activityGTPase activatorCaenorhabditis elegansC. elegansRGS genesDouble mutantProtein signalingGTPase activityProteinElegansBiological purposesRegulatorAppropriate behavioral responsesEggs
1996
EGL-10 Regulates G Protein Signaling in the C. elegans Nervous System and Shares a Conserved Domain with Many Mammalian Proteins
Koelle M, Horvitz H. EGL-10 Regulates G Protein Signaling in the C. elegans Nervous System and Shares a Conserved Domain with Many Mammalian Proteins. Cell 1996, 84: 115-125. PMID: 8548815, DOI: 10.1016/s0092-8674(00)80998-8.Peer-Reviewed Original ResearchMeSH KeywordsAllelesAmino Acid SequenceAnimalsBase SequenceCaenorhabditis elegansCaenorhabditis elegans ProteinsConserved SequenceFungal ProteinsGene DosageGenes, HelminthGTP-Binding ProteinsMammalsMolecular Sequence DataMusclesMutationNervous SystemNervous System Physiological PhenomenaOvumProteinsRatsRGS ProteinsSerotoninSignal TransductionYeastsConceptsEGL-10G proteinsNematode C. elegansG protein signalingMammalian genesGOA-1Mammalian proteinsC. elegansConserved domainProtein signalingNegative regulatorNeurotransmitter signalingProteinSignalingDose-dependent mannerPathwaySst2pElegansYeastGenesNervous systemDose-dependent fashionRegulatorCertain periodic behaviorsActivity