2021
The neural G protein Gαo tagged with GFP at an internal loop is functional in Caenorhabditis elegans
Kumar S, Olson AC, Koelle MR. The neural G protein Gαo tagged with GFP at an internal loop is functional in Caenorhabditis elegans. G3: Genes, Genomes, Genetics 2021, 11: jkab167. PMID: 34003969, PMCID: PMC8496287, DOI: 10.1093/g3journal/jkab167.Peer-Reviewed Original ResearchConceptsGreen fluorescent proteinCaenorhabditis elegansGenetic analysisHeterotrimeric G proteinsG protein GαoInternal loopC. elegansProtein complexesBiochemical purificationEpitope tagPlasma membraneAlpha subunitMolecular mechanismsFluorescent proteinGenetic studiesElegansGαoG proteinsEgg layingTransgenic expressionBiochemical studiesGαo proteinsBody morphologyProteinNeurotransmitter release
2005
Caenorhabditus elegans Arrestin Regulates Neural G Protein Signaling and Olfactory Adaptation and Recovery*
Palmitessa A, Hess HA, Bany IA, Kim YM, Koelle MR, Benovic JL. Caenorhabditus elegans Arrestin Regulates Neural G Protein Signaling and Olfactory Adaptation and Recovery*. Journal Of Biological Chemistry 2005, 280: 24649-24662. PMID: 15878875, DOI: 10.1074/jbc.m502637200.Peer-Reviewed Original ResearchMeSH KeywordsAllelesAmino Acid SequenceAnimalsAnimals, Genetically ModifiedArrestinBenzaldehydesBlotting, NorthernCaenorhabditis elegansCell LineCells, CulturedChemotaxisClathrinCOS CellsDiacetylEndocytosisExonsGreen Fluorescent ProteinsGTP-Binding ProteinsHumansImmunohistochemistryModels, GeneticMolecular Sequence DataMutationNeuronsOdorantsOlfactory PathwaysPentanolsPhenotypePhylogenyProtein BindingProtein Structure, TertiarySequence Analysis, DNASignal TransductionTime FactorsConceptsARR-1Receptor endocytosisG protein signalingG protein-coupled receptorsOlfactory adaptationVolatile odorantsProtein-coupled receptorsPotential mechanistic basisEndocytic machineryCaenorhabditis elegansNull mutantsHSN neuronsProtein signalingReceptor kinaseAdaptation defectRecovery defectArrestin functionChemosensory neuronsEnvironmental cuesBind proteinsMechanistic basisVivo linkTransgenic expressionArrestinNormal chemotaxis
2004
Activation of EGL-47, a Gαo-Coupled Receptor, Inhibits Function of Hermaphrodite-Specific Motor Neurons to Regulate Caenorhabditis elegans Egg-Laying Behavior
Moresco JJ, Koelle MR. Activation of EGL-47, a Gαo-Coupled Receptor, Inhibits Function of Hermaphrodite-Specific Motor Neurons to Regulate Caenorhabditis elegans Egg-Laying Behavior. Journal Of Neuroscience 2004, 24: 8522-8530. PMID: 15456826, PMCID: PMC6729914, DOI: 10.1523/jneurosci.1915-04.2004.Peer-Reviewed Original ResearchConceptsHermaphrodite-specific neuronsEgg-laying behaviorTransmembrane domainEgg-laying defectsEgg-laying musclesDominant mutationsHSN motor neuronsFluorescent protein transgeneSixth transmembrane domainExtracellular N-terminusEgg-laying frequencyMotor neuronsN-terminusG proteinsTransgenic expressionGenesCaenorhabditisMild defectsReceptor isoformsInhibits functionMutationsMultiple receptorsEggsReceptorsNumber of neuronsGenetic Analysis of RGS Protein Function in Caenorhabditis elegans
Chase DL, Koelle MR. Genetic Analysis of RGS Protein Function in Caenorhabditis elegans. Methods In Enzymology 2004, 389: 305-320. PMID: 15313573, DOI: 10.1016/s0076-6879(04)89018-9.Peer-Reviewed Original ResearchConceptsRGS proteinsC. elegansG proteinsRGS protein functionStructure/function studiesG protein geneCaenorhabditis elegansGalpha mutantsClose homologProtein functionGalpha proteinsElegansGenetic analysisDetailed protocolTransgenic expressionProteinMost mammaliansMutantsFunction studiesOrthologsCaenorhabditisHomologMammalianGenesOrganisms