2013
Isolation and characterization of biliary epithelial and stromal cells from resected human cholangiocarcinoma: A novel in vitro model to study tumor-stroma interactions
MASSANI M, STECCA T, FABRIS L, CARATOZZOLO E, RUFFOLO C, FURLANETTO A, MORTON S, CADAMURO M, STRAZZABOSCO M, BASSI N. Isolation and characterization of biliary epithelial and stromal cells from resected human cholangiocarcinoma: A novel in vitro model to study tumor-stroma interactions. Oncology Reports 2013, 30: 1143-1148. PMID: 23807641, DOI: 10.3892/or.2013.2568.Peer-Reviewed Original ResearchMeSH KeywordsBile Duct NeoplasmsBile Ducts, IntrahepaticBiomarkers, TumorCell CommunicationCholangiocarcinomaCoculture TechniquesEpithelial-Mesenchymal TransitionFibroblastsFlow CytometryFluorescent Antibody TechniqueHumansImmunoenzyme TechniquesImmunomagnetic SeparationNeoplasm GradingStromal CellsTumor Cells, CulturedConceptsHuman biliary epithelial cellsTumor-stroma interactionsCancer-associated fibroblastsStromal cellsOrganotypic co-culture modelPrimary culturesTumor cell originMesenchymal cell markersBiliary epithelial cellsCCA cell linesRat cholangiocarcinomaCo-culture modelDevastating malignancySurgical resectionBile ductPresent studySurgical specimensDesmoplastic reactionCholangiocarcinomaCell originHuman cholangiocarcinomaCell markersFluorescent immunocytochemistryEpithelial cellsCK7
2011
Epithelial–Mesenchymal Interactions in Biliary Diseases
Fabris L, Strazzabosco M. Epithelial–Mesenchymal Interactions in Biliary Diseases. Seminars In Liver Disease 2011, 31: 011-032. PMID: 21344348, PMCID: PMC3729030, DOI: 10.1055/s-0031-1272832.Peer-Reviewed Original ResearchConceptsReactive cholangiocytesChemo/cytokinesBile secretory functionLiver repair mechanismsDe novo expressionDifferent mesenchymal cell typesMost cholangiopathiesCell typesPortal fibrosisPortal infiltratesLiver diseaseBiliary diseaseUnderlying molecular mechanismsBiliary epitheliumDifferent etiologiesLiver repairSecretory functionNovo expressionCholangiopathyBiliary cellsCentral mechanismsMesenchymal cell typesMesenchymal propertiesEndothelial cellsGrowth factor