2023
Intrahepatic Cholangiocarcinoma Developing in Patients with Metabolic Syndrome Is Characterized by Osteopontin Overexpression in the Tumor Stroma
Cadamuro M, Sarcognato S, Camerotto R, Girardi N, Lasagni A, Zanus G, Cillo U, Gringeri E, Morana G, Strazzabosco M, Campello E, Simioni P, Guido M, Fabris L. Intrahepatic Cholangiocarcinoma Developing in Patients with Metabolic Syndrome Is Characterized by Osteopontin Overexpression in the Tumor Stroma. International Journal Of Molecular Sciences 2023, 24: 4748. PMID: 36902188, PMCID: PMC10003180, DOI: 10.3390/ijms24054748.Peer-Reviewed Original ResearchMeSH KeywordsBile Duct NeoplasmsBile Ducts, IntrahepaticCholangiocarcinomaHumansMetabolic SyndromeNon-alcoholic Fatty Liver DiseaseOsteopontinConceptsMetS patientsMetabolic syndromeNon-alcoholic fatty liver disease/non-alcoholic steatohepatitisNon-alcoholic steatohepatitisIntrahepatic cholangiocarcinoma developmentPutative therapeutic targetDeposition of osteopontinCell-like phenotypeBiliary tumorigenesisExtracellular matrix depositionVascular complicationsSurgical resectionIntrahepatic cholangiocarcinomaICCA cellsOverexpression of osteopontinPredictive biomarkersLiver tumorsCommon conditionHuCCT-1Tumor stromaCholangiocarcinoma developmentPeritumoral areaTherapeutic targetBiliary differentiationOsteopontin overexpression
2022
Locoregional Therapy in the Management of Intrahepatic Cholangiocarcinoma: Is There Sufficient Evidence to Guide Current Clinical Practice?
Wang Y, Strazzabosco M, Madoff DC. Locoregional Therapy in the Management of Intrahepatic Cholangiocarcinoma: Is There Sufficient Evidence to Guide Current Clinical Practice? Current Oncology Reports 2022, 24: 1741-1750. PMID: 36255606, PMCID: PMC10878124, DOI: 10.1007/s11912-022-01338-5.Peer-Reviewed Original ResearchMeSH KeywordsBile Duct NeoplasmsBile Ducts, IntrahepaticCholangiocarcinomaHepatectomyHumansRetrospective StudiesConceptsLocoregional therapyIntrahepatic cholangiocarcinomaManagement of ICCFuture prospective randomized studiesComparable survival outcomesSafety of hepatectomyProspective Randomized StudyLocal tumor controlNon-surgical approachCurrent clinical practicePurpose of reviewUnresectable diseaseSurgical candidatesSystemic therapyVenous embolizationRandomized studyTransarterial embolizationDismal prognosisMultimodal treatmentSafety profileSurvival outcomesAblative therapyTumor controlPreoperative hypertrophyLiver lobeInflammatory pathways and cholangiocarcioma risk mechanisms and prevention
Cadamuro M, Strazzabosco M. Inflammatory pathways and cholangiocarcioma risk mechanisms and prevention. Advances In Cancer Research 2022, 156: 39-73. PMID: 35961707, PMCID: PMC10916841, DOI: 10.1016/bs.acr.2022.02.001.Peer-Reviewed Original ResearchMeSH KeywordsBile Duct NeoplasmsBile Ducts, IntrahepaticCholangiocarcinomaCholangitis, SclerosingHumansRisk FactorsConceptsDevelopment of cholangiocarcinomaNonalcoholic fatty liver diseaseAdequate therapeutic treatmentPrimary sclerosing cholangitisFatty liver diseasePro-inflammatory mechanismsMain risk factorsProdromal diseaseSclerosing cholangitisCaroli's diseaseMetabolic syndromeChronic cholangiopathiesLiver diseasePoor prognosisInflammatory pathwaysBiliary treeCCA developmentRisk factorsImmune responseImmunological responseTherapeutic targetCholangiocarcinomaFluke infestationRole of cellExtrahepatic areas
2013
Platelet‐derived growth factor‐D and Rho GTPases regulate recruitment of cancer‐associated fibroblasts in cholangiocarcinoma
Cadamuro M, Nardo G, Indraccolo S, Dall'Olmo L, Sambado L, Moserle L, Franceschet I, Colledan M, Massani M, Stecca T, Bassi N, Morton S, Spirli C, Fiorotto R, Fabris L, Strazzabosco M. Platelet‐derived growth factor‐D and Rho GTPases regulate recruitment of cancer‐associated fibroblasts in cholangiocarcinoma. Hepatology 2013, 58: 1042-1053. PMID: 23505219, PMCID: PMC3732815, DOI: 10.1002/hep.26384.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntineoplastic AgentsBenzamidesBile Duct NeoplasmsBile Ducts, IntrahepaticCell Line, TumorCell MovementCell ProliferationCells, CulturedCholangiocarcinomaEpithelial-Mesenchymal TransitionFibroblastsHeterograftsHumansImatinib MesylateIn Vitro TechniquesLymphokinesMaleMiceMice, SCIDPiperazinesPlatelet-Derived Growth FactorPyrimidinesRho GTP-Binding ProteinsSignal TransductionConceptsCancer-associated fibroblastsPlatelet-derived growth factorEpithelial-mesenchymal transitionCCA cellsSecretion of PDGFRole of PDGFGrowth factorAbundant stromal reactionAlpha-smooth muscle actinPDGF-D expressionNovel therapeutic approachesPotential therapeutic targetSmooth muscle actinCCA cell linesPDGF-D signalingFibroblast migrationC-Jun N-terminal kinaseEMT biomarkersImmunodeficient miceStromal reactionTherapeutic approachesStroma interactionsTherapeutic targetCholangiocarcinomaMesenchymal markersVascular biology of the biliary epithelium
Morell CM, Fabris L, Strazzabosco M. Vascular biology of the biliary epithelium. Journal Of Gastroenterology And Hepatology 2013, 28: 26-32. PMID: 23855292, PMCID: PMC3721432, DOI: 10.1111/jgh.12022.Peer-Reviewed Original ResearchMeSH KeywordsAngiopoietinsAnimalsAutocrine CommunicationBile Duct DiseasesBile Ducts, IntrahepaticEpithelial CellsEpitheliumHumansLiverLiver Diseases, AlcoholicLiver RegenerationNeovascularization, PathologicParacrine CommunicationPlatelet-Derived Growth FactorRatsSignal TransductionVascular Endothelial Growth Factor AConceptsBile ductIntrahepatic bile ductsHepatic arteryPeribiliary plexusUnderlying molecular mechanismsArterial supplyLiver repairNormal organ physiologyLiver pathophysiologyVascular cell typesPathophysiological settingsVascular structuresStrong associationVascular biologyDifferent vascular cell typesCholangiocytesAngiogenic signalsLiver developmentCell typesMolecular mechanismsOrgan physiologyDuctAssociationCross talkIsolation and characterization of biliary epithelial and stromal cells from resected human cholangiocarcinoma: A novel in vitro model to study tumor-stroma interactions
MASSANI M, STECCA T, FABRIS L, CARATOZZOLO E, RUFFOLO C, FURLANETTO A, MORTON S, CADAMURO M, STRAZZABOSCO M, BASSI N. Isolation and characterization of biliary epithelial and stromal cells from resected human cholangiocarcinoma: A novel in vitro model to study tumor-stroma interactions. Oncology Reports 2013, 30: 1143-1148. PMID: 23807641, DOI: 10.3892/or.2013.2568.Peer-Reviewed Original ResearchMeSH KeywordsBile Duct NeoplasmsBile Ducts, IntrahepaticBiomarkers, TumorCell CommunicationCholangiocarcinomaCoculture TechniquesEpithelial-Mesenchymal TransitionFibroblastsFlow CytometryFluorescent Antibody TechniqueHumansImmunoenzyme TechniquesImmunomagnetic SeparationNeoplasm GradingStromal CellsTumor Cells, CulturedConceptsHuman biliary epithelial cellsTumor-stroma interactionsCancer-associated fibroblastsStromal cellsOrganotypic co-culture modelPrimary culturesTumor cell originMesenchymal cell markersBiliary epithelial cellsCCA cell linesRat cholangiocarcinomaCo-culture modelDevastating malignancySurgical resectionBile ductPresent studySurgical specimensDesmoplastic reactionCholangiocarcinomaCell originHuman cholangiocarcinomaCell markersFluorescent immunocytochemistryEpithelial cellsCK7Notch signaling regulates tubular morphogenesis during repair from biliary damage in mice
Fiorotto R, Raizner A, Morell CM, Torsello B, Scirpo R, Fabris L, Spirli C, Strazzabosco M. Notch signaling regulates tubular morphogenesis during repair from biliary damage in mice. Journal Of Hepatology 2013, 59: 124-130. PMID: 23500150, PMCID: PMC3777645, DOI: 10.1016/j.jhep.2013.02.025.Peer-Reviewed Original ResearchMeSH Keywords1-NaphthylisothiocyanateAmyloid Precursor Protein SecretasesAnimalsBile Ducts, IntrahepaticCalcium-Binding ProteinsImmunoglobulin J Recombination Signal Sequence-Binding ProteinIntercellular Signaling Peptides and ProteinsJagged-1 ProteinLiver RegenerationMembrane ProteinsMiceMice, Inbred C57BLMice, KnockoutMorphogenesisPyridinesReceptor, Notch2RNA, Small InterferingSerrate-Jagged ProteinsSignal TransductionStem CellsConceptsWild-type miceHepatic progenitor cellsBiliary damageType miceProgenitor cellsDuctular reactionΓ-secretase inhibitor treatmentTubule formationNotch signalingNotch-2 receptorRBP-JkBiliary repairMature ductsLiver-specific defectCKO miceInhibitor treatmentAbstractTextMiceNotch inhibitionNotch-1Jagged-1Notch-2ANITAIMSSOX-9
2011
Nuclear expression of S100A4 calcium‐binding protein increases cholangiocarcinoma invasiveness and metastasization
Fabris L, Cadamuro M, Moserle L, Dziura J, Cong X, Sambado L, Nardo G, Sonzogni A, Colledan M, Furlanetto A, Bassi N, Massani M, Cillo U, Mescoli C, Indraccolo S, Rugge M, Okolicsanyi L, Strazzabosco M. Nuclear expression of S100A4 calcium‐binding protein increases cholangiocarcinoma invasiveness and metastasization. Hepatology 2011, 54: 890-899. PMID: 21618579, PMCID: PMC3753582, DOI: 10.1002/hep.24466.Peer-Reviewed Original ResearchMeSH KeywordsAgedAnimalsApoptosisBile Duct NeoplasmsBile Ducts, IntrahepaticCell MovementCell NucleusCell ProliferationCholangiocarcinomaFemaleHumansMaleMatrix Metalloproteinase 2Matrix Metalloproteinase 9MiceMiddle AgedNeoplasm InvasivenessNeoplasm MetastasisPrognosisS100 Calcium-Binding Protein A4S100 ProteinsConceptsSurgical resectionCCA cellsNuclear expressionCCA patientsMetastatic propertiesSevere combined immunodeficiency miceTFK-1Time of surgeryRole of S100A4Log-rank testCombined immunodeficiency miceExpression of S100A4EGI-1 cellsHuman CCA cell linesPotential therapeutic targetMMP-9 secretionCCA cell linesHuman liver samplesCholangiocarcinoma invasivenessNuclear S100A4Severe prognosisPatient survivalPoor prognosisNeoplastic ductsImmunodeficiency mice
2010
Cholangiocarcinoma in Italy: A national survey on clinical characteristics, diagnostic modalities and treatment. Results from the “Cholangiocarcinoma” committee of the Italian Association for the Study of Liver disease
Alvaro D, Bragazzi MC, Benedetti A, Fabris L, Fava G, Invernizzi P, Marzioni M, Nuzzo G, Strazzabosco M, Stroffolini T, committee F. Cholangiocarcinoma in Italy: A national survey on clinical characteristics, diagnostic modalities and treatment. Results from the “Cholangiocarcinoma” committee of the Italian Association for the Study of Liver disease. Digestive And Liver Disease 2010, 43: 60-65. PMID: 20580332, DOI: 10.1016/j.dld.2010.05.002.Peer-Reviewed Original ResearchMeSH KeywordsAbdominal PainAgedBile Duct NeoplasmsBile Ducts, ExtrahepaticBile Ducts, IntrahepaticCholangiocarcinomaCholangiopancreatography, Endoscopic RetrogradeCholangiopancreatography, Magnetic ResonanceData CollectionDiarrheaFatigueFemaleHepatitis CHumansItalyJaundiceLiver CirrhosisMaleMiddle AgedNauseaTomography, X-Ray ComputedUltrasonographyVomitingWeight LossConceptsClinical characteristicsIH-CCAAsymptomatic presentationEH-CCACoexistence of cirrhosisCurative surgical treatmentTissue-proven diagnosisNational surveyCurative intentSurgical treatmentViral cirrhosisLiver diseaseOpen surgeryDiagnostic managementDiagnostic modalitiesCholangiocarcinoma casesCholangiocarcinomaYounger ageItalian AssociationCirrhosisDifferent imaging proceduresImaging proceduresDiagnosisTreatmentAge
2000
Pathophysiology of the intrahepatic biliary epithelium
Strazzabosco M, Spirlì C, Okolicsanyi L. Pathophysiology of the intrahepatic biliary epithelium. Journal Of Gastroenterology And Hepatology 2000, 15: 244-253. PMID: 10764023, DOI: 10.1046/j.1440-1746.2000.02091.x.Peer-Reviewed Original ResearchMeSH KeywordsAbsorptionApoptosisBile Duct DiseasesBile Ducts, IntrahepaticBiological TransportCell DivisionEpithelial CellsEpitheliumGastrointestinal HormonesHumansNeuropeptidesConceptsIntrahepatic biliary epitheliumBiliary epitheliumIntrahepatic bile duct epitheliumChronic cholestatic disorderBile duct epitheliumCholangiocyte functionBasic disease mechanismsPortal inflammationBiliary atresiaGastrointestinal hormonesCholestatic disordersPathophysiological pointBiliary treeCholangiocyte pathophysiologyImmune regulationPharmacological approachesNormal epitheliumCholangiocyte proliferationCholangiopathyDuct epitheliumInfectious agentsImportant causeBile acidsCystic fibrosisImmunoglobulin A.
1997
New insights into cholangiocyte physiology
Strazzabosco M. New insights into cholangiocyte physiology. Journal Of Hepatology 1997, 27: 945-952. PMID: 9382988, DOI: 10.1016/s0168-8278(97)80338-8.Peer-Reviewed Original ResearchNa+‐dependent and ‐independent Cl−/HCO exchange mediate cellular HCO transport in cultured human intrahepatic bile duct cells
Strazzabosco M, Joplin R, Zsembery A, Wallace L, Spirli C, Fabris L, Granato A, Rossanese A, Poci C, Neuberger J, Okolicsanyi L, Crepaldi G. Na+‐dependent and ‐independent Cl−/HCO exchange mediate cellular HCO transport in cultured human intrahepatic bile duct cells. Hepatology 1997, 25: 976-985. PMID: 9096607, DOI: 10.1002/hep.510250431.Peer-Reviewed Original ResearchConceptsEpithelial membrane antigenIntracellular acid loadHuman cholangiocytesAcid loadIntrahepatic bile duct cellsFactor VIII-related antigenIntracellular cyclic adenosine monophosphate (cAMP) concentrationsBiliary HCO3- secretionPediatric liver transplantationAdministration of agentsCyclic adenosine monophosphate concentrationsHuman hepatocyte growth factorVIII-related antigenBile duct cellsNormal liver tissueBiliary epithelial cellsCl- channel inhibitorsHepatocyte growth factorAdenosine monophosphate concentrationsIntracellular cAMP concentrationLiver transplantationReduced graftAbsence of HCO3Biliary treeAcid loader
1994
Effect of ursodeoxycholic acid on intracellular pH in a bile duct epithelium‐like cell line
Strazzabosco M, Poci C, Spirlí C, Sartori L, Knuth A, Crepaldi G. Effect of ursodeoxycholic acid on intracellular pH in a bile duct epithelium‐like cell line. Hepatology 1994, 19: 145-154. PMID: 8276351, DOI: 10.1002/hep.1840190124.Peer-Reviewed Original ResearchConceptsUrsodeoxycholic acidDuct epitheliumUnconjugated ursodeoxycholic acidSK-ChA-1 cellsBile duct epitheliumSK-ChA-1Acid-induced increaseFluorescent indicator 2Human cholangiocarcinoma cell linesCell linesIntracellular pH regulatory mechanismsCholangiocarcinoma cell linesCl/HCO3 exchangePreincubation of cellsAbsence of HCO3Furosemide administrationPH regulatory mechanismsAcid/base transportersDuct levelUDCAHCO3- exchange
1989
Quantitative assessment of canalicular bile formation in isolated hepatocyte couplets using microscopic optical planimetry.
Gautam A, Ng O, Strazzabosco M, Boyer J. Quantitative assessment of canalicular bile formation in isolated hepatocyte couplets using microscopic optical planimetry. Journal Of Clinical Investigation 1989, 83: 565-573. PMID: 2913052, PMCID: PMC303716, DOI: 10.1172/jci113919.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBileBile CanaliculiBile Ducts, IntrahepaticLiverMicroscopy, ElectronRatsRats, Inbred StrainsTaurocholic AcidUrsodeoxycholic AcidConceptsIsolated rat hepatocyte couplets