2016
Menthol decreases oral nicotine aversion in C57BL/6 mice through a TRPM8-dependent mechanism
Fan L, Balakrishna S, Jabba SV, Bonner PE, Taylor SR, Picciotto MR, Jordt SE. Menthol decreases oral nicotine aversion in C57BL/6 mice through a TRPM8-dependent mechanism. Tobacco Control 2016, 25: ii50. PMID: 27698211, PMCID: PMC5496986, DOI: 10.1136/tobaccocontrol-2016-053209.Peer-Reviewed Original ResearchConceptsSmokeless tobacco productsWild-type miceOral nicotineC57BL/6 miceTobacco productsNicotine aversionAversive effectsTwo-bottle choice drinkingTrigeminal sensory neuronsEffect of mentholProduct useMenthol concentrationOral mentholSensory neuronsAversive concentrationsMenthol receptorOral cavityMenthol effectsRespiratory irritationMiceNicotineTRPM8Oral irritantsIrritantsAversive tasteCaMKII Phosphorylation of TARPγ-8 Is a Mediator of LTP and Learning and Memory
Park J, Chávez AE, Mineur YS, Morimoto-Tomita M, Lutzu S, Kim KS, Picciotto MR, Castillo PE, Tomita S. CaMKII Phosphorylation of TARPγ-8 Is a Mediator of LTP and Learning and Memory. Neuron 2016, 92: 75-83. PMID: 27667007, PMCID: PMC5059846, DOI: 10.1016/j.neuron.2016.09.002.Peer-Reviewed Original ResearchConceptsCaMKII phosphorylation siteCaMKII substratePhosphorylation sitesDependent protein kinase IIProtein kinase IIReceptor-dependent activationNMDA receptor-dependent activationProtein phosphorylationAMPAR-mediated transmissionKinase IICaMKII-dependent enhancementLong-term potentiationCaMKII phosphorylationCellular mechanismsPhosphorylationMolecular targetsAMPA receptorsCrucial mediatorSynaptic plasticityMemory formationSynaptic insertionEssential stepSynaptic transmissionActivity-dependent strengtheningBasal transmission
2015
Multiple Nicotinic Acetylcholine Receptor Subtypes in the Mouse Amygdala Regulate Affective Behaviors and Response to Social Stress
Mineur YS, Fote GM, Blakeman S, Cahuzac EL, Newbold SA, Picciotto MR. Multiple Nicotinic Acetylcholine Receptor Subtypes in the Mouse Amygdala Regulate Affective Behaviors and Response to Social Stress. Neuropsychopharmacology 2015, 41: 1579-1587. PMID: 26471256, PMCID: PMC4832019, DOI: 10.1038/npp.2015.316.Peer-Reviewed Original ResearchConceptsDepression-like behaviorBasolateral amygdalaΑ7 nAChRsCholinergic signalingMultiple nicotinic acetylcholine receptor subtypesNon-selective nAChR antagonist mecamylamineNicotinic acetylcholine receptor activityNicotinic acetylcholine receptor subtypesStress-mediated behaviorsAntidepressant-like effectsAcetylcholine receptor activityC-Fos immunoreactivityNAChR antagonist mecamylamineAcetylcholine receptor subtypesEffects of nicotineMajor depressive disorderSocial defeat stressAnxiety-like behaviorPre-clinical studiesHuman clinical trialsModels of anxietyMouse behavioral modelsHypercholinergic stateAntagonist mecamylamineLocal infusion
2013
Galanin‐induced decreases in nucleus accumbens/striatum excitatory postsynaptic potentials and morphine conditioned place preference require both galanin receptor 1 and galanin receptor 2
Einstein EB, Asaka Y, Yeckel MF, Higley MJ, Picciotto MR. Galanin‐induced decreases in nucleus accumbens/striatum excitatory postsynaptic potentials and morphine conditioned place preference require both galanin receptor 1 and galanin receptor 2. European Journal Of Neuroscience 2013, 37: 1541-1549. PMID: 23387435, PMCID: PMC3648588, DOI: 10.1111/ejn.12151.Peer-Reviewed Original ResearchConceptsExcitatory postsynaptic potentialsAbility of galaninSame receptor subtypeReceptor subtypesKnockout miceMorphine CPPPostsynaptic potentialsPlace preferenceAmplitude of EPSPsGalanin receptor 1Galanin receptor subtypesGalanin receptor 2Striatal brain slicesWild-type miceWhole-cell recordingsPotential cellular mechanismsNeuropeptide galaninEPSP amplitudeOpiate rewardBrain slicesNucleus accumbensDorsal striatumExcitatory signalingGalaninReceptor 2
2012
The Synaptic Adhesion Molecule SynCAM 1 Contributes to Cocaine Effects on Synapse Structure and Psychostimulant Behavior
Giza JI, Jung Y, Jeffrey RA, Neugebauer NM, Picciotto MR, Biederer T. The Synaptic Adhesion Molecule SynCAM 1 Contributes to Cocaine Effects on Synapse Structure and Psychostimulant Behavior. Neuropsychopharmacology 2012, 38: 628-638. PMID: 23169347, PMCID: PMC3572459, DOI: 10.1038/npp.2012.226.Peer-Reviewed Original ResearchConceptsNucleus accumbensDrugs of abuseSynCAM 1Cocaine effectsSynapse structureMushroom-type spinesExcitatory synapse numberMedium spiny neuronsAddiction-related behaviorsKO miceSpiny neuronsStubby spinesSynapse numberAdhesion molecule proteinsExcitatory synapsesCocaine administrationTrans-synaptic interactionsKnockout micePsychostimulant effectsNAc synapsesStructural remodelingTherapeutic interventionsSynaptic cleftPsychostimulant cocaineSynapse developmentConstitutive knockout of the membrane cytoskeleton protein beta adducin decreases mushroom spine density in the nucleus accumbens but does not prevent spine remodeling in response to cocaine
Jung Y, Mulholland PJ, Wiseman SL, Chandler LJ, Picciotto MR. Constitutive knockout of the membrane cytoskeleton protein beta adducin decreases mushroom spine density in the nucleus accumbens but does not prevent spine remodeling in response to cocaine. European Journal Of Neuroscience 2012, 37: 1-9. PMID: 23106536, PMCID: PMC3538958, DOI: 10.1111/ejn.12037.Peer-Reviewed Original ResearchConceptsNucleus accumbensCocaine administrationSpine densitySynaptic plasticityMature mushroom-shaped spinesMushroom spine densityDaily cocaine administrationMushroom-shaped spinesChronic cocaine administrationOverall spine densityElevated locomotor activityExperience-dependent remodelingLipophilic dye DiINAc slicesWT miceMushroom spinesKO miceCocaine treatmentDiolistic labelingSynaptic remodelingBrain slicesBrain areasConstitutive knockoutMature spinesLocomotor activityImpaired auditory discrimination learning following perinatal nicotine exposure or β2 nicotinic acetylcholine receptor subunit deletion
Horst NK, Heath CJ, Neugebauer NM, Kimchi EY, Laubach M, Picciotto MR. Impaired auditory discrimination learning following perinatal nicotine exposure or β2 nicotinic acetylcholine receptor subunit deletion. Behavioural Brain Research 2012, 231: 170-180. PMID: 22433585, PMCID: PMC3334440, DOI: 10.1016/j.bbr.2012.03.002.Peer-Reviewed Original ResearchConceptsAuditory stimulus processingStimulus processingAuditory discrimination learningUnderlying neurobiological mechanismsAuditory discrimination paradigmLow rates (DRL) taskPerinatal nicotine exposureAuditory discriminationGeneral deficitDiscrimination learningTask performanceNicotine exposureDiscrimination impairmentNeurobiological mechanismsMouse perinatal developmentDiscrimination paradigmRate taskDifferential reinforcementAdministration of nicotineTaskAuditory pathwayLearningNicotinic acetylcholine receptorsMaternal smokingProcessing
2011
Plasticity of Prefrontal Attention Circuitry: Upregulated Muscarinic Excitability in Response to Decreased Nicotinic Signaling Following Deletion of α5 or β2 Subunits
Tian MK, Bailey CD, De Biasi M, Picciotto MR, Lambe EK. Plasticity of Prefrontal Attention Circuitry: Upregulated Muscarinic Excitability in Response to Decreased Nicotinic Signaling Following Deletion of α5 or β2 Subunits. Journal Of Neuroscience 2011, 31: 16458-16463. PMID: 22072695, PMCID: PMC3240894, DOI: 10.1523/jneurosci.3600-11.2011.Peer-Reviewed Original ResearchMeSH KeywordsAcetylcholineAconitineAge FactorsAnalysis of VarianceAnimalsAtropineDihydro-beta-ErythroidineIntracellular Signaling Peptides and ProteinsMaleMembrane PotentialsMiceMice, KnockoutNeuronal PlasticityNeuronsNeuropeptidesNeurotransmitter AgentsNicotineNicotinic AntagonistsOrexinsPatch-Clamp TechniquesPrefrontal CortexReceptors, MuscarinicReceptors, NicotinicSignal TransductionUp-RegulationConceptsLayer VI neuronsNicotinic receptorsCholinergic excitationCholinergic receptorsPrefrontal cortexExcitatory muscarinic receptorsPrefrontal attention circuitryMuscarinic cholinergic receptorsMuscarinic acetylcholine receptorsAcute brain slicesWild-type miceWhole-cell recordingsΒ2 subunitNicotinic receptor subunitsMedial prefrontal cortexPyramidal neuronsMuscarinic receptorsNicotinic signalingLayer VIAttention circuitryCholinergic stimulationBrain slicesNicotinic stimulationAcetylcholine receptorsTiming of excitationGalanin negatively modulates opiate withdrawal via galanin receptor 1
Holmes FE, Armenaki A, Iismaa TP, Einstein EB, Shine J, Picciotto MR, Wynick D, Zachariou V. Galanin negatively modulates opiate withdrawal via galanin receptor 1. Psychopharmacology 2011, 220: 619-625. PMID: 21969124, PMCID: PMC3324978, DOI: 10.1007/s00213-011-2515-x.Peer-Reviewed Original ResearchConceptsGalanin receptor 1Chronic morphine administrationMorphine administrationLocus coeruleusGalanin expressionOpiate withdrawalReceptor 1Distinct G protein-coupled receptorsPrecipitation of withdrawalAction of morphineDoses of morphineWild-type miceTransgenic mouse lineG protein-coupled receptorsExpress galaninGalanin levelsWild-type controlsProtein-coupled receptorsNeuropeptide galaninMorphine dependenceWithdrawal signsOpiate dependenceGalaninTransgenic miceGalR2 geneReduced locomotor responses to cocaine in ghrelin-deficient mice
Abizaid A, Mineur YS, Roth RH, Elsworth JD, Sleeman MW, Picciotto MR, Horvath TL. Reduced locomotor responses to cocaine in ghrelin-deficient mice. Neuroscience 2011, 192: 500-506. PMID: 21699961, DOI: 10.1016/j.neuroscience.2011.06.001.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCocaineDopamineDopamine Uptake InhibitorsGhrelinMiceMice, Inbred C57BLMice, Inbred DBAMice, KnockoutMotor ActivityConceptsGhrelin KO miceWT miceDopamine releaseLocomotor activityBehavioral effectsDopamine metabolite concentrationsGhrelin-deficient miceLocomotor-stimulating effectsDopamine cell activityEffects of cocaineMesolimbic dopaminergic systemWild-type littermatesOrexigenic hormoneChronic injectionsDopamine utilizationDaily injectionsStriatal dopamineMesolimbic systemWT littermatesDopaminergic systemDaily cocaineFood intakeRewarding effectsPsychostimulant effectsLocomotor responseNicotine Decreases Food Intake Through Activation of POMC Neurons
Mineur YS, Abizaid A, Rao Y, Salas R, DiLeone RJ, Gündisch D, Diano S, De Biasi M, Horvath TL, Gao XB, Picciotto MR. Nicotine Decreases Food Intake Through Activation of POMC Neurons. Science 2011, 332: 1330-1332. PMID: 21659607, PMCID: PMC3113664, DOI: 10.1126/science.1201889.Peer-Reviewed Original ResearchConceptsFood intakePOMC neuronsNicotine decreases food intakeDecrease food intakePro-opiomelanocortin (POMC) neuronsΑ3β4 nicotinic acetylcholine receptorsHypothalamic melanocortin systemNicotine-induced decreasesMelanocortin-4 receptorNicotinic acetylcholine receptorsAnorexic effectDecrease appetiteSmoking cessationSynaptic mechanismsMelanocortin systemNovel treatmentsBody weightAcetylcholine receptorsNeurobiological mechanismsNeuronsIntakeSubsequent activationAppetiteActivationReceptorsAn Instructive Role for Patterned Spontaneous Retinal Activity in Mouse Visual Map Development
Xu HP, Furman M, Mineur YS, Chen H, King SL, Zenisek D, Zhou ZJ, Butts DA, Tian N, Picciotto MR, Crair MC. An Instructive Role for Patterned Spontaneous Retinal Activity in Mouse Visual Map Development. Neuron 2011, 70: 1115-1127. PMID: 21689598, PMCID: PMC3119851, DOI: 10.1016/j.neuron.2011.04.028.Peer-Reviewed Original ResearchConceptsSpontaneous retinal activityRetinal activityRetinal ganglion cell projectionsEye-specific segregationGanglion cell projectionsSpontaneous retinal wavesActivity-dependent refinementRetinal ganglion cellsMouse visual systemComplex neural circuitsEye of originRetinal wavesGanglion cellsRetinotopic refinementNeuronal activitySpontaneous activityMammalian visual systemAcetylcholine receptorsNeuronal connectivityMammalian brainNeural circuitsOverall activity levelsActivity levelsBrainVisual systemStriatal‐enriched protein tyrosine phosphatase (STEP) knockout mice have enhanced hippocampal memory
Venkitaramani DV, Moura PJ, Picciotto MR, Lombroso PJ. Striatal‐enriched protein tyrosine phosphatase (STEP) knockout mice have enhanced hippocampal memory. European Journal Of Neuroscience 2011, 33: 2288-2298. PMID: 21501258, PMCID: PMC3118976, DOI: 10.1111/j.1460-9568.2011.07687.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBehavior, AnimalFocal Adhesion Kinase 2HippocampusMemoryMiceMice, Inbred C57BLMice, KnockoutMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3PhosphorylationProtein Tyrosine Phosphatases, Non-ReceptorReceptors, AMPAReceptors, N-Methyl-D-AspartateSynaptic TransmissionConceptsStriatal-enriched protein tyrosine phosphataseSTEP KO miceProtein tyrosine phosphataseBrain-specific phosphataseProline-rich tyrosine kinaseEffect of deletionN-methyl-D-aspartate receptorsERK1/2 substratesNR1/NR2B N‐Methyl‐d‐Aspartate ReceptorsPotential molecular mechanismsTyrosine phosphataseSignaling proteinsTyrosine phosphorylationDownstream effectorsKinase 1/2Molecular mechanismsTyrosine kinaseFunctional importanceKnockout micePhosphorylationSTEP knockout miceSynaptic strengtheningIsoxazole propionic acid (AMPA) receptorsSynaptosomal expressionRegulation
2010
Mice lacking the galanin gene show decreased sensitivity to nicotine conditioned place preference
Neugebauer NM, Henehan RM, Hales CA, Picciotto MR. Mice lacking the galanin gene show decreased sensitivity to nicotine conditioned place preference. Pharmacology Biochemistry And Behavior 2010, 98: 87-93. PMID: 21172385, PMCID: PMC3030658, DOI: 10.1016/j.pbb.2010.12.015.Peer-Reviewed Original ResearchConceptsGal-/- miceAcute nicotine administrationNicotine CPPPlace preferenceNicotine administrationRewarding effectsRole of galaninEffects of nicotineNucleus accumbens shellClass of drugsAmphetamine place preferenceDrugs of abuseGalanin signalingNeuropeptide galaninCPP chamberAlcohol drinkingNicotine rewardSignificant CPPExtracellular signal-related kinaseAccumbens shellGalanin peptideHigh doseSystem activationAlcohol preferenceSignal-related kinaseLocomotion and Self-Administration Induced by Cocaine in 129/OlaHsd Mice Lacking Galanin
Brabant C, Kuschpel AS, Picciotto MR. Locomotion and Self-Administration Induced by Cocaine in 129/OlaHsd Mice Lacking Galanin. Behavioral Neuroscience 2010, 124: 828-838. PMID: 21038934, PMCID: PMC3058554, DOI: 10.1037/a0021221.Peer-Reviewed Original ResearchConceptsGal KO miceCocaine-induced hyperactivityGalanin receptor agonistCocaine-induced hyperlocomotionSpontaneous motor activityGalanin knockout miceEffects of cocaineDrug takersDoses of cocaineDrugs of abuseSelf-administer cocaineGalanin systemReceptor agonistLocomotor effectsKnockout miceGalaninIntravenous cocaineMotor activityFixed ratio scheduleSelf-AdministrationGenetic deletionMiceCocaineDifferent schedulesRatio scheduleGalanin and Addiction
Picciotto MR. Galanin and Addiction. EXS 2010, 102: 195-208. PMID: 21299070, DOI: 10.1007/978-3-0346-0228-0_14.Peer-Reviewed Original ResearchConceptsGalanin receptorsFood intakeOpiate rewardDrug rewardAbility of neuropeptidesMesolimbic dopamine systemDrugs of abuseGalanin agonistsNoradrenergic neuronsDrug withdrawalStriatal slicesWithdrawal signsAnalgesic propertiesLocus coeruleusHypothalamic functionDopamine releaseGalaninDopamine systemNovel treatmentsPlace preferenceStress-related pathwaysFiring ratePotential targetWithdrawalIntakeModulation of ethanol consumption by genetic and pharmacological manipulation of nicotinic acetylcholine receptors in mice
Kamens HM, Andersen J, Picciotto MR. Modulation of ethanol consumption by genetic and pharmacological manipulation of nicotinic acetylcholine receptors in mice. Psychopharmacology 2010, 208: 613-626. PMID: 20072781, PMCID: PMC2901400, DOI: 10.1007/s00213-009-1759-1.Peer-Reviewed Original ResearchConceptsEffects of vareniclineEthanol consumptionNicotinic acetylcholine receptorsEthanol intakeAcetylcholine receptorsPharmacological manipulationΒ2 subunitRole of nAChRsTwo-bottle choice paradigmWild-type micePartial agonist vareniclineSubunit knockout miceMesolimbic dopamine systemNicotinic controlVarenicline doseAlcohol drinkingAgonist vareniclineKnockout miceDopamine systemNicotine responseChallenge studiesVareniclineNAChRsReceptor subunitsMice
2009
The membrane cytoskeletal protein adducin is phosphorylated by protein kinase C in D1 neurons of the nucleus accumbens and dorsal striatum following cocaine administration
Lavaur J, Mineur YS, Picciotto MR. The membrane cytoskeletal protein adducin is phosphorylated by protein kinase C in D1 neurons of the nucleus accumbens and dorsal striatum following cocaine administration. Journal Of Neurochemistry 2009, 111: 1129-1137. PMID: 19780900, PMCID: PMC2810345, DOI: 10.1111/j.1471-4159.2009.06405.x.Peer-Reviewed Original ResearchMeSH KeywordsAnalysis of VarianceAnimalsBenzazepinesBenzophenanthridinesCalmodulin-Binding ProteinsCocaineCorpus StriatumDopamine AntagonistsDopamine Uptake InhibitorsDose-Response Relationship, DrugEnzyme InhibitorsGene Expression RegulationGreen Fluorescent ProteinsMaleMiceMice, Inbred C57BLMice, KnockoutNeuronsNucleus AccumbensPhosphorylationProtein Kinase CRacloprideReceptors, Dopamine D1Time FactorsConceptsProtein kinase CAdducin phosphorylationKinase CActin-binding proteinsFamily of proteinsPhosphorylation of adducinCytoskeletal protein adducinActin dynamicsCytoskeletal rearrangementsPhosphorylation stateCytoskeletal proteinsAdducinF-actinPhosphorylationNeuronal cytoskeletonCellular architectureProteinSynaptic functionMorphological changesCytoskeletonMedium spiny neuronsSpectrinRegimen of cocaineActinRegulation
2008
Effects of galanin on cocaine-mediated conditioned place preference and ERK signaling in mice
Narasimhaiah R, Kamens HM, Picciotto MR. Effects of galanin on cocaine-mediated conditioned place preference and ERK signaling in mice. Psychopharmacology 2008, 204: 95-102. PMID: 19099295, PMCID: PMC2872184, DOI: 10.1007/s00213-008-1438-7.Peer-Reviewed Original ResearchConceptsVentral tegmental areaEffects of galaninNucleus accumbensNeuropeptide galaninPlace preferenceThreshold doseEffects of morphineMesolimbic dopamine systemMesolimbic dopaminergic pathwaySystemic cocaine injectionPlace preference paradigmCocaine-induced activationDrugs of abuseAmphetamine reinforcementWild-type controlsExtracellular signal-regulated kinase (ERK) activityTegmental areaCocaine injectionCocaine responseDopaminergic pathwaysConclusionsThese dataGalaninRewarding effectsDopamine systemDrug reinforcementRole of β2-containing nicotinic acetylcholine receptors in auditory event-related potentials
Rudnick ND, Koehler C, Picciotto MR, Siegel SJ. Role of β2-containing nicotinic acetylcholine receptors in auditory event-related potentials. Psychopharmacology 2008, 202: 745-751. PMID: 18931833, DOI: 10.1007/s00213-008-1358-6.Peer-Reviewed Original ResearchConceptsAuditory event-related potentialsEffects of nicotineWild-type miceN40 amplitudeNicotine effectsSpecific nicotinic acetylcholine receptor subtypesNicotinic acetylcholine receptor subtypesΒ2-containing receptorsAcetylcholine receptor subtypesEvent-related potentialsSensory processingΒ2 knockout miceSchizophrenic individualsNicotinic acetylcholine receptorsModulates sensory processingAuditory ERP responsesNicotine administrationReceptor subtypesCA3 regionKnockout miceTherapeutic targetClick stimuliN40 componentStereotaxic implantationAcetylcholine receptors