Recombinant adeno-associated virus (AAV-CFTR) vectors do not integrate in a site-specific fashion in an immortalized epithelial cell line.
Kearns W, Afione S, Fulmer S, Pang M, Erikson D, Egan M, Landrum M, Flotte T, Cutting G. Recombinant adeno-associated virus (AAV-CFTR) vectors do not integrate in a site-specific fashion in an immortalized epithelial cell line. Gene Therapy 1996, 3: 748-55. PMID: 8875221.Peer-Reviewed Original ResearchConceptsIB3-1 cellsWild-type AAVEpithelial cell lineMetaphase spreadsSite-specific fashionCFTR cDNACell linesLow molecular weight DNA fractionCF bronchial epithelial cell lineHuman chromosome 19Immortalized epithelial cell lineWeight DNA fractionMetaphase chromosome preparationsMetaphase chromosome spreadsBronchial epithelial cell lineDifferent chromosomesAAV-CFTR vectorCDNA integrationChromosome spreadsChromosome 19Genomic DNASite-specific mannerDNA fractionChromosome preparationsCFTR expression