2015
bSUM: A bead-supported unilamellar membrane system facilitating unidirectional insertion of membrane proteins into giant vesicles
Zheng H, Lee S, Llaguno MC, Jiang QX. bSUM: A bead-supported unilamellar membrane system facilitating unidirectional insertion of membrane proteins into giant vesicles. The Journal Of General Physiology 2015, 147: 77-93. PMID: 26712851, PMCID: PMC4692488, DOI: 10.1085/jgp.201511448.Peer-Reviewed Original ResearchConceptsMembrane proteinsIon channelsLipid compositionLipid bilayersMembrane systemLipid-protein interactionsVitro membrane systemSingle-molecule imagingVoltage-gated ion channelsGiant vesiclesUnilamellar naturePlanar electrodesPlanar lipid bilayersSuch proteinsMicroscopic imagingBiophysical analysisFast gating kineticsUnidirectional insertionProteinChannel activityProtein orientationGating kineticsModel systemBilayersSpecific ligands
2011
Massive endocytosis driven by lipidic forces originating in the outer plasmalemmal monolayer: a new approach to membrane recycling and lipid domains
Fine M, Llaguno MC, Lariccia V, Lin MJ, Yaradanakul A, Hilgemann DW. Massive endocytosis driven by lipidic forces originating in the outer plasmalemmal monolayer: a new approach to membrane recycling and lipid domains. The Journal Of General Physiology 2011, 137: 137-154. PMID: 21242300, PMCID: PMC3032378, DOI: 10.1085/jgp.201010469.Peer-Reviewed Original ResearchConceptsMassive endocytosisPlasma membraneBaby hamster kidneyFM 4HEK293 cellsActin cytoskeleton remodelingNonionic detergentMembrane rufflesProtein cyclingCytoskeleton remodelingMembrane recyclingCytoplasmic sideNa/Ca exchangerTriphosphate hydrolysisCytoplasmic ATPEndocytosisMembrane monolayersCell surfaceLipid domainsG protein cyclingOuter monolayerMembrane tracerVesiclesAmphipathic drugsNP-40
2010
Massive calcium–activated endocytosis without involvement of classical endocytic proteins
Lariccia V, Fine M, Magi S, Lin MJ, Yaradanakul A, Llaguno MC, Hilgemann DW. Massive calcium–activated endocytosis without involvement of classical endocytic proteins. The Journal Of General Physiology 2010, 137: 111-132. PMID: 21187336, PMCID: PMC3010057, DOI: 10.1085/jgp.201010468.Peer-Reviewed Original ResearchActin CytoskeletonAdenosine TriphosphateAnimalsBeta-CyclodextrinsCalcineurinCalciumCell MembraneCells, CulturedCeramidesCholesterolClathrinCricetinaeDynaminsElectric CapacitanceEndocytosisEstrenesExocytosisGuanosine 5'-O-(3-Thiotriphosphate)HEK293 CellsHumansInositol Polyphosphate 5-PhosphatasesKidneyLipidsMembrane ProteinsNaphthalenesPhosphoric Monoester HydrolasesPolyaminesPyridinium CompoundsPyronesPyrrolidinonesQuaternary Ammonium CompoundsSodium-Calcium ExchangerSphingomyelin Phosphodiesterase