2022
Mitochondrial ATP synthase c-subunit leak channel triggers cell death upon loss of its F1 subcomplex
Mnatsakanyan N, Park HA, Wu J, He X, Llaguno MC, Latta M, Miranda P, Murtishi B, Graham M, Weber J, Levy RJ, Pavlov EV, Jonas EA. Mitochondrial ATP synthase c-subunit leak channel triggers cell death upon loss of its F1 subcomplex. Cell Death & Differentiation 2022, 29: 1874-1887. PMID: 35322203, PMCID: PMC9433415, DOI: 10.1038/s41418-022-00972-7.Peer-Reviewed Original ResearchConceptsMitochondrial permeability transitionATP synthase c-subunitCell deathMitochondrial ATP synthaseChannel activityCellular energy productionLeak channelsVoltage-gated ion channelsF1 subcomplexATP synthaseC subunitInner membraneProkaryotic hostsCell stressPermeability transitionIon channelsGating mechanismOsmotic changesLarge conductanceC-ringChannels triggersNeuronal deathF1SubcomplexOsmotic gradient
2014
Chemically functionalized carbon films for single molecule imaging
Llaguno MC, Xu H, Shi L, Huang N, Zhang H, Liu Q, Jiang QX. Chemically functionalized carbon films for single molecule imaging. Journal Of Structural Biology 2014, 185: 405-417. PMID: 24457027, PMCID: PMC3990355, DOI: 10.1016/j.jsb.2014.01.006.Peer-Reviewed Original ResearchConceptsCarbon surfaceNanometer-thick carbon filmsBiological complexesCarbon filmsIndividual macromolecular complexesFunctionalized filmsBioactive ligandsChemical linkageSingle-molecule imagingSubnanogram levelActive siteStrong oxidationNanomolar levelsDNA/RNAMolecule imagingNegative-stain EMElectron microscopic imagingSelective enrichmentComplexesLigandsMacromolecular complexesFilmsProtein GSubnanomolar concentrationsLong-term storage
2012
Phase transitions in the assembly of multivalent signalling proteins
Li P, Banjade S, Cheng HC, Kim S, Chen B, Guo L, Llaguno M, Hollingsworth JV, King DS, Banani SF, Russo PS, Jiang QX, Nixon BT, Rosen MK. Phase transitions in the assembly of multivalent signalling proteins. Nature 2012, 483: 336-340. PMID: 22398450, PMCID: PMC3343696, DOI: 10.1038/nature10879.Peer-Reviewed Original ResearchMeSH KeywordsActin-Related Protein 2-3 ComplexAdaptor Proteins, Signal TransducingBinding SitesBiopolymersFluorescence Recovery After PhotobleachingHeLa CellsHumansLigandsMembrane ProteinsMultiprotein ComplexesOncogene ProteinsPhase TransitionPhosphorylationProline-Rich Protein DomainsProtein Structure, QuaternaryProteinsSignal TransductionSrc Homology DomainsWiskott-Aldrich Syndrome Protein, Neuronal
2011
Massive endocytosis driven by lipidic forces originating in the outer plasmalemmal monolayer: a new approach to membrane recycling and lipid domains
Fine M, Llaguno MC, Lariccia V, Lin MJ, Yaradanakul A, Hilgemann DW. Massive endocytosis driven by lipidic forces originating in the outer plasmalemmal monolayer: a new approach to membrane recycling and lipid domains. The Journal Of General Physiology 2011, 137: 137-154. PMID: 21242300, PMCID: PMC3032378, DOI: 10.1085/jgp.201010469.Peer-Reviewed Original ResearchConceptsMassive endocytosisPlasma membraneBaby hamster kidneyFM 4HEK293 cellsActin cytoskeleton remodelingNonionic detergentMembrane rufflesProtein cyclingCytoskeleton remodelingMembrane recyclingCytoplasmic sideNa/Ca exchangerTriphosphate hydrolysisCytoplasmic ATPEndocytosisMembrane monolayersCell surfaceLipid domainsG protein cyclingOuter monolayerMembrane tracerVesiclesAmphipathic drugsNP-40
2010
Massive calcium–activated endocytosis without involvement of classical endocytic proteins
Lariccia V, Fine M, Magi S, Lin MJ, Yaradanakul A, Llaguno MC, Hilgemann DW. Massive calcium–activated endocytosis without involvement of classical endocytic proteins. The Journal Of General Physiology 2010, 137: 111-132. PMID: 21187336, PMCID: PMC3010057, DOI: 10.1085/jgp.201010468.Peer-Reviewed Original ResearchActin CytoskeletonAdenosine TriphosphateAnimalsBeta-CyclodextrinsCalcineurinCalciumCell MembraneCells, CulturedCeramidesCholesterolClathrinCricetinaeDynaminsElectric CapacitanceEndocytosisEstrenesExocytosisGuanosine 5'-O-(3-Thiotriphosphate)HEK293 CellsHumansInositol Polyphosphate 5-PhosphatasesKidneyLipidsMembrane ProteinsNaphthalenesPhosphoric Monoester HydrolasesPolyaminesPyridinium CompoundsPyronesPyrrolidinonesQuaternary Ammonium CompoundsSodium-Calcium ExchangerSphingomyelin Phosphodiesterase