2014
Chemically functionalized carbon films for single molecule imaging
Llaguno MC, Xu H, Shi L, Huang N, Zhang H, Liu Q, Jiang QX. Chemically functionalized carbon films for single molecule imaging. Journal Of Structural Biology 2014, 185: 405-417. PMID: 24457027, PMCID: PMC3990355, DOI: 10.1016/j.jsb.2014.01.006.Peer-Reviewed Original ResearchConceptsCarbon surfaceNanometer-thick carbon filmsBiological complexesCarbon filmsIndividual macromolecular complexesFunctionalized filmsBioactive ligandsChemical linkageSingle-molecule imagingSubnanogram levelActive siteStrong oxidationNanomolar levelsDNA/RNAMolecule imagingNegative-stain EMElectron microscopic imagingSelective enrichmentComplexesLigandsMacromolecular complexesFilmsProtein GSubnanomolar concentrationsLong-term storage
2012
Phase transitions in the assembly of multivalent signalling proteins
Li P, Banjade S, Cheng HC, Kim S, Chen B, Guo L, Llaguno M, Hollingsworth JV, King DS, Banani SF, Russo PS, Jiang QX, Nixon BT, Rosen MK. Phase transitions in the assembly of multivalent signalling proteins. Nature 2012, 483: 336-340. PMID: 22398450, PMCID: PMC3343696, DOI: 10.1038/nature10879.Peer-Reviewed Original ResearchMeSH KeywordsActin-Related Protein 2-3 ComplexAdaptor Proteins, Signal TransducingBinding SitesBiopolymersFluorescence Recovery After PhotobleachingHeLa CellsHumansLigandsMembrane ProteinsMultiprotein ComplexesOncogene ProteinsPhase TransitionPhosphorylationProline-Rich Protein DomainsProtein Structure, QuaternaryProteinsSignal TransductionSrc Homology DomainsWiskott-Aldrich Syndrome Protein, Neuronal