2006
Mapping the Binding Site on CD8β for MHC Class I Reveals Mutants with Enhanced Binding
Devine L, Thakral D, Nag S, Dobbins J, Hodsdon ME, Kavathas PB. Mapping the Binding Site on CD8β for MHC Class I Reveals Mutants with Enhanced Binding. The Journal Of Immunology 2006, 177: 3930-3938. PMID: 16951356, DOI: 10.4049/jimmunol.177.6.3930.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBinding SitesCD8 AntigensChlorocebus aethiopsCOS CellsHistocompatibility Antigens Class IHumansMiceMolecular Sequence DataMutagenesisConceptsMHC class IClass IT cellsT cell responsesEffective immune responseT cell recognitionT cell hybridomasT cell membraneImmune responseCoreceptor functionCell responsesCell hybridomasInfected cellsCell recognitionIdentified mutationsCD8alphaCoreceptorCD8betaCDR3 loopsCellsEnhanced bindingCD8ImmunotherapyCD8alphabetaCD8β
2004
Location of the epitope for an anti-CD8α antibody 53.6.7 which enhances CD8α-MHC class I interaction indicates antibody stabilization of a higher affinity CD8 conformation
Devine L, Hodsdon ME, Daniels MA, Jameson SC, Kavathas PB. Location of the epitope for an anti-CD8α antibody 53.6.7 which enhances CD8α-MHC class I interaction indicates antibody stabilization of a higher affinity CD8 conformation. Immunology Letters 2004, 93: 123-130. PMID: 15158607, DOI: 10.1016/j.imlet.2004.02.002.Peer-Reviewed Original ResearchAnimalsAntibodies, MonoclonalAntibody AffinityAntibody SpecificityBiotinylationCD8 AntigensChlorocebus aethiopsCOS CellsEgg ProteinsEpitope MappingEscherichia coliFlow CytometryH-2 AntigensHistocompatibility Antigens Class IMiceModels, MolecularMutagenesis, Site-DirectedOligopeptidesOvalbuminPeptide FragmentsProtein BindingProtein ConformationTransfection
2000
Human CD8β, But Not Mouse CD8β, Can Be Expressed in the Absence of CD8α as a ββ Homodimer
Devine L, Kieffer L, Aitken V, Kavathas P. Human CD8β, But Not Mouse CD8β, Can Be Expressed in the Absence of CD8α as a ββ Homodimer. The Journal Of Immunology 2000, 164: 833-838. PMID: 10623829, DOI: 10.4049/jimmunol.164.2.833.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCD8 AntigensCell LineCOS CellsCysteineDimerizationHistocompatibility Antigens Class IHumansImmunoglobulinsMiceMice, TransgenicProtein BindingProtein IsoformsProtein Structure, TertiarySpecies SpecificityTransfectionConceptsChimeric proteinIg domainsCell-cell adhesion assaysBeta-beta dimersCD8 alphaForms of CD8CD8 betaTyrosine kinase p56lckCOS-7 cellsMHC class IDisulfide-linked homodimerCD8 alpha alpha homodimerCysteine residuesKinase p56lckCD8 alpha alphaAlpha alpha homodimerLack of expressionAlpha polypeptide chainPolypeptide chainMature T cellsCell surfaceClass IBeta-IgBeta betaAbility of CD8
1999
Molecular analysis of protein interactions mediating the function of the cell surface protein CD8
Devine L, Kavathas P. Molecular analysis of protein interactions mediating the function of the cell surface protein CD8. Immunologic Research 1999, 19: 201-210. PMID: 10493174, DOI: 10.1007/bf02786488.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCD8 AntigensHistocompatibility Antigens Class IHumansLigandsMiceReceptors, Antigen, T-CellSignal TransductionT-LymphocytesConceptsProtein interactionsForms of CD8Such protein interactionsTyrosine kinase p56lckDisulfide-linked homodimerCell surface glycoproteinMolecular detailsKinase p56lckMutational analysisMolecular analysisFunctional differencesCoreceptor CD8ProteinHistocompatibility complex class IAffinity measurementsMajor histocompatibility complex class IComplex class ICellsP56lckHomodimerHeterodimersCrystal structureClass IGlycoproteinInteractionOrientation of the Ig domains of CD8 alpha beta relative to MHC class I.
Devine L, Sun J, Barr M, Kavathas P. Orientation of the Ig domains of CD8 alpha beta relative to MHC class I. The Journal Of Immunology 1999, 162: 846-51. PMID: 9916707, DOI: 10.4049/jimmunol.162.2.846.Peer-Reviewed Original Research