2016
Imaging Functional Nucleic Acid Delivery to Skin
Kaspar RL, Hickerson RP, González-González E, Flores MA, Speaker TP, Rogers FA, Milstone LM, Contag CH. Imaging Functional Nucleic Acid Delivery to Skin. Methods In Molecular Biology 2016, 1372: 1-24. PMID: 26530911, DOI: 10.1007/978-1-4939-3148-4_1.Peer-Reviewed Original ResearchConceptsNucleic acid deliveryNucleic acid-based therapiesAcid deliveryAcid-based therapiesFunctional deliveryFunctional nucleic acid deliveryTherapeutic nucleic acidsNucleic acidsOptical imaging technologiesReal-time monitoringTriplex nucleic acidsInteraction of lightMonogenic skin diseasesLight-tissue interactionsGene editingGene silencingOptical imagingNoninvasive imaging technologyTopical deliveryTime monitoringImaging technologyReporter geneLayered structureGeneral strategyEnzymatic reactions
2011
Visualization of plasmid delivery to keratinocytes in mouse and human epidermis
González-González E, Kim YC, Speaker TJ, Hickerson RP, Spitler R, Birchall JC, Lara MF, Hu RH, Liang Y, Kirkiles-Smith N, Prausnitz MR, Milstone LM, Contag CH, Kaspar RL. Visualization of plasmid delivery to keratinocytes in mouse and human epidermis. Scientific Reports 2011, 1: 158. PMID: 22355673, PMCID: PMC3240989, DOI: 10.1038/srep00158.Peer-Reviewed Original ResearchConceptsIdeal target organHuman skin xenograftsAccessibility of skinSkin delivery technologiesNon-invasive intravital imagingIntradermal injectionSkin xenograftsClinical utilityTarget organsPatient-friendly deliveryMouse skinBioluminescence imagingSkin model systemIntravital imagingNoninvasive assayTransfection of cellsIntravital fluorescenceHuman epidermisIndividual keratinocytesKeratinocytesSkinNucleic acid-based therapeuticsMiceReporter plasmidDelivery technologies