2016
The X-Ray Crystal Structure of the Keratin 1-Keratin 10 Helix 2B Heterodimer Reveals Molecular Surface Properties and Biochemical Insights into Human Skin Disease
Bunick CG, Milstone LM. The X-Ray Crystal Structure of the Keratin 1-Keratin 10 Helix 2B Heterodimer Reveals Molecular Surface Properties and Biochemical Insights into Human Skin Disease. Journal Of Investigative Dermatology 2016, 137: 142-150. PMID: 27595935, PMCID: PMC5514376, DOI: 10.1016/j.jid.2016.08.018.Peer-Reviewed Original Research
2015
Crystal Structure of Human Profilaggrin S100 Domain and Identification of Target Proteins Annexin II, Stratifin, and HSP27
Bunick CG, Presland RB, Lawrence OT, Pearton DJ, Milstone LM, Steitz TA. Crystal Structure of Human Profilaggrin S100 Domain and Identification of Target Proteins Annexin II, Stratifin, and HSP27. Journal Of Investigative Dermatology 2015, 135: 1801-1809. PMID: 25760235, PMCID: PMC4466033, DOI: 10.1038/jid.2015.102.Peer-Reviewed Original ResearchMeSH Keywords14-3-3 ProteinsAnnexin A2Biomarkers, TumorCells, CulturedCrystallizationEpidermal CellsEpidermisExoribonucleasesFilaggrin ProteinsHSP27 Heat-Shock ProteinsHumansIntermediate Filament ProteinsKeratinocytesProtein BindingProtein TransportS100 ProteinsSensitivity and SpecificitySpectrometry, FluorescenceConceptsÅ resolution crystal structureProtein-protein interactionsHuman profilaggrinCalcium-binding domainKeratinocyte terminal differentiationMolecular functionsProtein interactionsTerminal domainShock protein 27Cell envelopeIdentification of targetsN-terminusMolecular approachesTerminal differentiationNormal epidermal barrierHydrophobic pocketSpecific functionsAnnexin IIStable dimerMolecular interfaceProtein 27Proteolytic productsProfilaggrinProteinCrystal structure
2012
Inhibition of CD44 Gene Expression in Human Skin Models, Using Self-Delivery Short Interfering RNA Administered by Dissolvable Microneedle Arrays
Lara MF, González-González E, Speaker TJ, Hickerson RP, Leake D, Milstone LM, Contag CH, Kaspar RL. Inhibition of CD44 Gene Expression in Human Skin Models, Using Self-Delivery Short Interfering RNA Administered by Dissolvable Microneedle Arrays. Human Gene Therapy 2012, 23: 816-823. PMID: 22480249, PMCID: PMC3413896, DOI: 10.1089/hum.2011.211.Peer-Reviewed Original ResearchConceptsTransgenic mouse modelInhibition of CD44CD44-specific antibodiesCD44 mRNA levelsDissolvable microneedle arraysGene expressionMouse modelXenograft modelSkin disordersCD44 gene expressionHuman skin modelTarget cellsMRNA levelsHuman skin equivalentsKeratinocyte membraneTreatmentInhibitionStratum corneumTarget gene expressionSiRNASkin equivalentsEpidermal equivalentsMicroneedle arraysSkin modelExpression
2010
Development of Quantitative Molecular Clinical End Points for siRNA Clinical Trials
Hickerson RP, Leachman SA, Pho LN, Gonzalez-Gonzalez E, Smith FJ, McLean WH, Contag CH, Leake D, Milstone LM, Kaspar RL. Development of Quantitative Molecular Clinical End Points for siRNA Clinical Trials. Journal Of Investigative Dermatology 2010, 131: 1029-1036. PMID: 21191405, DOI: 10.1038/jid.2010.372.Peer-Reviewed Original ResearchConceptsClinical trialsPachyonychia congenitaEnd pointPhase 1b clinical trialClinical end pointsRare skin disorderMolecular end pointsTypes of trialsImmune surveillancePatient-derived keratinocytesSkin disordersSustained inhibitionTreatment resultsRNAi-based therapeuticsRNA interferenceMRNA levelsTrialsTotal RNAHuman skinKeratinocyte culturesMRNAAmplifiable mRNATherapeuticsInhibitorsInhibition
2001
Photodegraded Nifedipine Stimulates Uptake and Retention of Iron in Human Epidermal Keratinocytes
Gruen A, Zhou J, Milstone L, Morton K. Photodegraded Nifedipine Stimulates Uptake and Retention of Iron in Human Epidermal Keratinocytes. Journal Of Investigative Dermatology 2001, 116: 774-777. PMID: 11348469, DOI: 10.1046/j.1523-1747.2001.01318.x.Peer-Reviewed Original ResearchConceptsHuman epidermal keratinocytesEpidermal keratinocytesD washout periodNifedipine treatmentDaily treatmentWashout periodNifedipineRetention of ironEpidermal desquamationIron uptakeKeratinocytesKeratinocyte monolayersSerum proteinsRelevant concentrationsToxic amountsEpidermal culturesTreatmentErythroid cells
1999
Growth and differentiation regulate CD44 expression on human keratinocytes
Zhou J, Haggerty J, Milstone L. Growth and differentiation regulate CD44 expression on human keratinocytes. In Vitro Cellular & Developmental Biology - Animal 1999, 35: 228-235. PMID: 10478803, DOI: 10.1007/s11626-999-0031-7.Peer-Reviewed Original ResearchMeSH KeywordsCell DifferentiationCell DivisionCells, CulturedEpidermal CellsEpidermisHumansHyaluronan ReceptorsKeratinocytesProteoglycansConceptsCD44 expressionBasal keratinocytesChondroitin sulfate proteoglycan formKeratan sulfate proteoglycanConfluent culturesPreconfluent monolayersDifferentiated cellsHyaluronan receptorWestern immunoblotCD44 proteinHuman keratinocytesCD44 familyKeratinocytesPreconfluent culturesSulfate proteoglycanDifferentiated keratinocytesCD44Core proteinSplice variants
1994
Epican, a heparan/chondroitin sulfate proteoglycan form of CD44, mediates cell-cell adhesion
Milstone L, Hough-Monroe L, Kugelman L, Bender J, Haggerty J. Epican, a heparan/chondroitin sulfate proteoglycan form of CD44, mediates cell-cell adhesion. Journal Of Cell Science 1994, 107: 3183-3190. PMID: 7699015, DOI: 10.1242/jcs.107.11.3183.Peer-Reviewed Original ResearchResponse of stratified cultures of human keratinocytes to disruption of proteoglycan synthesis by p‐nitrophenyl‐β‐D‐xylopyranoside
Haggerty J, Bretton R, Milstone L. Response of stratified cultures of human keratinocytes to disruption of proteoglycan synthesis by p‐nitrophenyl‐β‐D‐xylopyranoside. Journal Of Cellular Physiology 1994, 158: 39-46. PMID: 8263027, DOI: 10.1002/jcp.1041580106.Peer-Reviewed Original Research
1992
Human dermatosparaxis: a form of Ehlers-Danlos syndrome that results from failure to remove the amino-terminal propeptide of type I procollagen.
Smith L, Wertelecki W, Milstone L, Petty E, Seashore M, Braverman I, Jenkins T, Byers P. Human dermatosparaxis: a form of Ehlers-Danlos syndrome that results from failure to remove the amino-terminal propeptide of type I procollagen. American Journal Of Human Genetics 1992, 51: 235-44. PMID: 1642226, PMCID: PMC1682688.Peer-Reviewed Original ResearchConceptsAmino-terminal propeptideFragile skinType IConnective tissue disordersEhlers-Danlos syndromeLax jointsType VIIAffected animalsSkinChildrenCultured fibroblastsNormal cellsEhlers-Danlos syndrome type VIIPro alphaDisordersPropeptideAlphaDermatosparaxisProteolytic processingSepsisN-proteinaseCellsSyndromeCollagen fibrilsMethotrexate induces differentiation of human keratinocytes.
Schwartz P, Barnett S, Atillasoy E, Milstone L. Methotrexate induces differentiation of human keratinocytes. Proceedings Of The National Academy Of Sciences Of The United States Of America 1992, 89: 594-598. PMID: 1731329, PMCID: PMC48285, DOI: 10.1073/pnas.89.2.594.Peer-Reviewed Original ResearchConceptsEffect of methotrexateAbility of methotrexateProliferation of keratinocytesProportion of cellsMarkers of differentiationHuman epidermal keratinocytesMethotrexateTherapeutic actionEpidermal keratinocytesMarked increaseHuman keratinocytesKeratinocytesEnvelope proteinSDS/PAGE profilesInvolucrin synthesisNormal cellsTerminal differentiationTissue homeostasisThymine deoxyribonucleotidesDifferentiationCellsPsoriasisCell morphologyReproducible changesDose
1991
Glycosylation of Parathyroid Hormone-Related Peptide Secreted by Human Epidermal Keratinocytes*
WU T, SOIFER N, BURTIS W, MILSTONE L, STEWART A. Glycosylation of Parathyroid Hormone-Related Peptide Secreted by Human Epidermal Keratinocytes*. The Journal Of Clinical Endocrinology & Metabolism 1991, 73: 1002-1007. PMID: 1939513, DOI: 10.1210/jcem-73-5-1002.Peer-Reviewed Original ResearchMeSH KeywordsBlotting, WesternCells, CulturedChromatography, AffinityChromatography, High Pressure LiquidElectrophoresis, Polyacrylamide GelEpidermisGlycosylationHumansInfant, NewbornKeratinocytesMaleParathyroid Hormone-Related ProteinProtein Processing, Post-TranslationalProteinsRecombinant ProteinsImmunohistochemical Localization of Parathyroid Hormone-Related Protein (PTHRP) In Normal Human Skin
Atillasoy E, Burtis W, Milstone L. Immunohistochemical Localization of Parathyroid Hormone-Related Protein (PTHRP) In Normal Human Skin. Journal Of Investigative Dermatology 1991, 96: 277-280. PMID: 1991989, DOI: 10.1111/1523-1747.ep12464480.Peer-Reviewed Original ResearchConceptsParathyroid hormone-related peptideNormal human skinHormone-related peptideHuman keratinocytesHuman skinParathyroid hormoneSquamous differentiationAffinity-purified polyclonal antibodiesNumber of autocrineImmunohistochemical localizationParacrine functionEpithelial cellsViable portionKeratinocytesSkinPolyclonal antibodiesRecent studiesPeptidesAutocrineHormoneAntibodies
1990
Measurement of retinoid effects on epidermal renewal
Milstone L. Measurement of retinoid effects on epidermal renewal. Methods In Enzymology 1990, 190: 76-81. PMID: 2087197, DOI: 10.1016/0076-6879(90)90011-o.Chapters
1988
Local and Systemic Implications of Thymidine Catabolism by Human Keratinocytesa
SCHWARTZ P, REUVENI H, MILSTONE L. Local and Systemic Implications of Thymidine Catabolism by Human Keratinocytesa. Annals Of The New York Academy Of Sciences 1988, 548: 115-124. PMID: 2470288, DOI: 10.1111/j.1749-6632.1988.tb18798.x.ChaptersThymidine phosphorylase in human epidermal keratinocytes
Schwartz P, Milstone L. Thymidine phosphorylase in human epidermal keratinocytes. Biochemical Pharmacology 1988, 37: 353-355. PMID: 2449215, DOI: 10.1016/0006-2952(88)90740-x.Peer-Reviewed Original ResearchHuman Keratinocytes Catabolize Thymidine
Schwartz P, Kugelman L, Coifman Y, Hough L, Milstone L. Human Keratinocytes Catabolize Thymidine. Journal Of Investigative Dermatology 1988, 90: 8-12. PMID: 3335793, DOI: 10.1111/1523-1747.ep12462412.Peer-Reviewed Original ResearchConceptsHuman keratinocytesHuman neonatal foreskinHuman neonatal foreskin keratinocytesNeonatal foreskin keratinocytesCutaneous diseaseSoluble extractsGuinea pigsNeonatal foreskinKeratinocyte proliferationForeskin keratinocytesStratified culturesRate of incorporationAdult skinRadioactive thymidineKeratinocytesThymidine analogueAmount of thymidineExogenous thymidineSpecies differencesThymidineHuman fibroblastsSkinCatabolismDifferent tissuesCulture medium
1987
Skin-Derived Fibroblasts Respond to Human Parathyroid Hormone-Like Adenylate Cyclase-Stimulating Proteins*
WU T, INSOGNA K, HOUGH L, MILSTONE L, STEWART A. Skin-Derived Fibroblasts Respond to Human Parathyroid Hormone-Like Adenylate Cyclase-Stimulating Proteins*. The Journal Of Clinical Endocrinology & Metabolism 1987, 65: 105-109. PMID: 3034948, DOI: 10.1210/jcem-65-1-105.Peer-Reviewed Original ResearchConceptsPTH-like factorHumoral hypercalcemiaPTH analogsPTH-responsive adenylate cyclaseDermal fibroblast linesAdenylate cyclaseTumor-derived factorsAdenylate cyclase responseDermal fibroblastsAdenylate cyclase stimulationHuman dermal fibroblastsM bovine PTHDermal physiologyFibroblast linesHuman dermal fibroblast linesBovine PTHHigh prevalenceTumor extractsCyclase responseSkin-derived fibroblastsCyclase stimulationPTHKeratinocyte-conditioned mediumHuman tumorsApparent potency
1986
A Parathyroid Hormone-Like Protein from Cultured Human Keratinocytes
Merendino JJ, Insogna KL, Milstone LM, Broadus AE, Stewart AF. A Parathyroid Hormone-Like Protein from Cultured Human Keratinocytes. Science 1986, 231: 388-390. PMID: 2417317, DOI: 10.1126/science.2417317.Peer-Reviewed Original ResearchConceptsHumoral hypercalcemiaParathyroid hormoneHuman keratinocytesParathyroid hormone receptor agonistsParathyroid hormone-like proteinSquamous epithelial originExtracts of tumorsHormone receptor agonistsHormone-like proteinHormone-like factorsAdenylate cyclase activityRat osteosarcoma cellsAdenylate cyclase productionNative parathyroid hormoneCultured human keratinocytesNormal human keratinocytesReceptor agonistMalignant tumorsCyclic adenosine monophosphateHypercalcemiaEpithelial originKeratinocyte-conditioned mediumMalignancyCyclase activityCyclase production
1985
Heterogeneity of Basal Keratinocytes: Nonrandom Distribution of Thymidine-Labeled Basal Cells in Confluent Cultures Is Not a Technical Artifact
Milstone L, LaVigne J. Heterogeneity of Basal Keratinocytes: Nonrandom Distribution of Thymidine-Labeled Basal Cells in Confluent Cultures Is Not a Technical Artifact. Journal Of Investigative Dermatology 1985, 84: 504-507. PMID: 3998498, DOI: 10.1111/1523-1747.ep12273479.Peer-Reviewed Original Research
1984
Epidermal Cell Confluence and Implications for a Two-Step Mechanism of Wound Closure
Stenn K, Milstone L. Epidermal Cell Confluence and Implications for a Two-Step Mechanism of Wound Closure. Journal Of Investigative Dermatology 1984, 83: 445-447. PMID: 6209344, DOI: 10.1111/1523-1747.ep12273552.Peer-Reviewed Original Research